Untersuchungen zur Inaktivierung von HIV-1 in humanen Femurk�pfen durch Verwendung eines thermischen Desinfektionssystems (Lobator SD-1)

Abstract

The use of allogenic bone transplants in surgery has been greatly diminished owing to the risk of transmitting infectious diseases. This risk can be reduced by the use of a thermal disinfection system (Lobator SD-1). This is achieved by increasing the temperature to 80 degrees C, inactivating a number of bacterial and viral agents. In this study the decay of HIV at high temperature in the Lobator SD-1 was researched. In the center of human femoral heads 100 microliters of a highly concentrated suspension of free and cell-bound HIV (10(10)) was exposed to the thermal process at intervals of 5, 10, 20, 30, 40, 50 and 62 min. For the recultivation HUT-78 cells were used through titration of the virus suspension in ten-fold dilutions over ten dilution steps and incubation up to a maximum of 21 days. Evidence of the virus was checked through observing giant cell formations and quantitative determination of p24 antigen using an Elisa test. Linear virus inactivation was found based upon the time the virus was exposed to heat. After a treatment of 40 min in the disinfection system, total virus inactivation was achieved. The normal disinfection process time using Lobator SD-1 is 92 min. A temperature of 80 degrees C is reached after approximately 45 min. The results prove that this system totally inactivates HIV in human femoral heads.

Lyophilization Does Not Inactivate Infectious Retrovirus in Systemically Infected Bone and Tendon Allografts

“…21 In addition, excessive heat during the secondary drying process or during storage of freeze-dried tissues can also decrease viral viability. [39][40][41]53,62 Because residual moisture content and temperature can vary among freeze-drying protocols, 51 it is conceivable that the reported conflicting results, with regard to viral transmission after freeze-drying, may be a result of such variations in technique. The current AATB standards for freezedrying of connective tissues mandate only that residual moisture content shall not exceed 6% of the initial weight.…”

Endemic impact of human T cell leukemia virus type 1 screening in bone allografts

“…These findings emphasize the importance of screening donors for HTLV-1 prior to the use of their donations in transplantation (Schreiber et al 1996; von Garrel et al 1997). The limitation of this study was that we didn’t confirm the presence of viable cells including proviral DNA in our samples.…”

Ein klinisch-radiologischer Score für Femurkopftransplantate

“…Wissenschaftliche Untersuchungen zur klinisch-morphologischen Bewertung des FK zur Transplantation liegen derzeit nicht vor. In der Literatur sind vor allem Studien und Untersuchungen zu mikrobiologischen [ 9 , 19 , 22 , 23 ], immunologischen [ 13 , 26 ], infektionssicherheitsrelevanten [ 10 , 14 , 17 , 24 ] und ökonomisch-pharmazeutischen Fragestellungen [ 1 , 12 , 15 , 16 ] zu finden.…”