Untersuchungen über Wesen und Fehlerquellen des Toxoplasmose-Serofarbtestes nach Sabin und Feldman

A cytochemical investigation of the Sabin-Feldman phenomenon in Toxoplasma gondii and an explanation of its mechanism on this basis

“…The second possibility considered by Sabin & Feldman (1948) was a change in the permeability of the cell wall of the organism affected by the immunity process. This theory was supported by Westphal & Muhlpfordt (1950) who considered the reaction to be due to a change in the electrical potential relationship of the cell membrane surface caused bya colloidal accumulation of proteins. However, Sabin & Feldman (1948) discarded this point of view as modified Toxoplasma exhibited nuclear staining which showed that there was diffusion of the dye into the cytoplasm of the organism.…”

“…However, Sabin & Feldman (1948) discarded this point of view as modified Toxoplasma exhibited nuclear staining which showed that there was diffusion of the dye into the cytoplasm of the organism. On the other hand, Westphal & Muhlpfordt (1950) believed that only a small amount of dye penetrated into the Toxoplasma cell at a time and this deposited on the part of the cell with the greatest affinity, which was the nucleus, leaving the rest of the cell constituents unstained. However, they believed that the staining of the remaining cell constituents would follow in due course.…”