1964
DOI: 10.1002/hlca.19640470712
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Untersuchungen über die Reaktion von Peptiden mit Phenylisothiocyanat. Anwendung der Dünnschichtchromatographie zur Sequenzanalyse von Peptiden. 2. Mitteilung

Abstract: Nach einmaliger Umsetzung eines Peptids mit Phenylisothiocyanat lässt sich noch ein Teil des unveränderten Peptids dünnschichtchromatographisch nachweisen. Durch Wiederholung der Reaktion kann die Ausbeute an Phenylthiocarbamoyl‐Peptid er‐höht werden.

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Cited by 22 publications
(1 citation statement)
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“…Especially the p-bromo-substituted phenylthiohydantoins characterized by a significant double peak of a mass difference of 2 amu for the molecular ion peak facilitated identification and interpretation of the relevant mass spectra. Mass spectrometry is superior to other methods of analysis based on thin-layer chromatography [10,11] or gas-liquid chromatography [12,13], since identification is directly correlated to individual mass numbers of the phenylthiohydantoin under investigation. Electron impact mass spectrometry, however, suffered from the difficulty of cysteine identification.…”
Section: Introductionmentioning
confidence: 99%
“…Especially the p-bromo-substituted phenylthiohydantoins characterized by a significant double peak of a mass difference of 2 amu for the molecular ion peak facilitated identification and interpretation of the relevant mass spectra. Mass spectrometry is superior to other methods of analysis based on thin-layer chromatography [10,11] or gas-liquid chromatography [12,13], since identification is directly correlated to individual mass numbers of the phenylthiohydantoin under investigation. Electron impact mass spectrometry, however, suffered from the difficulty of cysteine identification.…”
Section: Introductionmentioning
confidence: 99%