Untersuchungen über die kernverhältnisse und morphologischen eigenschaften symbiontischer mikroorganismen bei verschiedenen insekten

Kolb, Gertrud

doi:10.1007/bf00389908

Cited by 20 publications

(13 citation statements)

References 114 publications

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“…These early studies had already revealed fascinating insights in the biology of the symbiosis of bacteria with ants of the genera Camponotus and Formica, but they remained inconclusive and controversial, e.g., regarding the question of how the bacteria enter the oocytes and whether the bacteria are also located in follicle or nurse cells surrounding the oocytes (3,6,10,13). For example, an invasion of bacteria from the midgut bacteriocytes was reported which was believed to be followed by massive infiltration of the bacteria into the nearby ovarioles (5).…”

Section: Discussionmentioning

confidence: 99%

“…Investigations conducted in the early 20th century suggested that in C. ligniperdus and F. fusca, the upper part of the ovaries contain cells loaded with bacteria. These cells are associated with the oocytes, and it was assumed that the bacteria in these cells invade the premature oocytes at some developmental stage (6,10,13,15). To gain further insight into the fate of the bacteria during the life span of their host animals, we investigated the distribution of the bacteria in various tissues of queens, workers, and males and during larval development by using light and electron microscopy and in situ hybridization techniques.…”

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confidence: 99%

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Tissue Localization of the Endosymbiotic Bacterium “ Candidatus Blochmannia floridanus” in Adults and Larvae of the Carpenter Ant Camponotus floridanus

Sauer

Dudaczek

Hölldobler³

et al. 2002

Appl Environ Microbiol

104

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The distribution of endosymbiotic bacteria in different tissues of queens, males, and workers of the carpenter ant Camponotus floridanus was investigated by light and electron microscopy and by in situ hybridization. A large number of bacteria could be detected in bacteriocytes within the midguts of workers, young virgin queens, and males. Large amounts of bacteria were also found in the oocytes of workers and queens. In contrast, bacteria were not present in oocyte-associated cells or in the spermathecae of mature queens, although occasionally a small number of bacteria could be detected in the testis follicles of males. Interestingly, the number of bacteriocytes in mature queens was strongly reduced and the bacteriocytes contained only very few or no bacteria at all, although the endosymbionts were present in huge amounts in the ovaries of the same animals. During embryogenesis of the deposited egg, the bacteria were concentrated in a ring of endodermal tissue destined to become the midgut in later developmental stages. However, during larval development, bacteria could also be detected in other tissues although to a lesser extent. Only in the last-instar larvae were bacteria found exclusively in the midgut tissue within typical bacteriocytes. Tetracycline and rifampin efficiently cleansed C. floridanus workers of their symbionts and the bacteriocytes of these animals still remained empty several months after treatment had ceased. Despite the lack of their endosymbionts, these adult animals were able to survive without any obvious negative effect under normal cultivation conditions.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Tissue Localization of the Endosymbiotic Bacterium “ Candidatus Blochmannia floridanus” in Adults and Larvae of the Carpenter Ant Camponotus floridanus

Sauer

Dudaczek

Hölldobler³

et al. 2002

Appl Environ Microbiol

104

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“…These bacteria are Gram-negative rods of variable length, large numbers of which reside in the cytoplasm of mycetocytes intercalated between normal epithelial cells (Dasch et al, 1984 ;Schro$ der et al, 1996). The same bacteria are also found in the cytoplasm of oocytes of queens and workers, which suggests a transovarial transmission route of the bacteria (Buchner, 1965 ;Kolb, 1959 ;Schro$ der et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Systematic relationships and cospeciation of bacterial endosymbionts and their carpenter ant host species: proposal of the new taxon Candidatus Blochmannia gen. nov.

Sauer

Stackebrandt

Gadau

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

177

141

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“…The bacterial endosymbionts reside in specialized cells, the bacteriocytes, which are intercalated between enterocytes of the midgut tissue of the host. In females, the bacteria also occur in the oocytes, leading to a vertical transmission of the bacteria (6,13,19,20). Blochmannia is closely related to other insect endosymbionts, like Wigglesworthia glossinidia, the endosymbiont of the tsetse fly Glossina brevipalpis, and Buchnera aphidicola, the endosymbiont of aphids (1,2,3,9,11,22).…”

mentioning

confidence: 99%

Relevance of the Endosymbiosis of Blochmannia floridanus and Carpenter Ants at Different Stages of the Life Cycle of the Host

Zientz¹,

Beyaert

Gross³

et al. 2006

Appl Environ Microbiol

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Expression of several genes possibly involved in the symbiotic relationship between the obligate intracellular endosymbiont Blochmannia floridanus and its ant host Camponotus floridanus was investigated at different developmental stages of the host by real-time quantitative PCR. These included a set of genes related to nitrogen metabolism (ureC, ureF, glnA, and speB) as well as genes involved in the synthesis of the aromatic amino acid tyrosine (tyrA, aspC, and hisC). The overall transcriptional activity of Blochmannia was found to be quite low during early developmental stages and to increase steadily with host age. However, a concerted peak of gene expression related to nitrogen recycling could be detected around the entire process of pupation, while expression of biosynthesis pathways for aromatic amino acids was elevated only during a short phase in pupation. These data suggest an important role of certain metabolic functions for the symbiotic interactions of the bacteria and an individual host organism in early phases of development. General relevance of Blochmannia for its ant host was tested in fostering experiments with worker groups of Camponotus floridanus, and their success in raising pupae from first-instar larvae was used as a fitness measure. Groups treated with antibiotics had a significantly reduced success in raising the brood in comparison to untreated control groups, indicating that the symbiosis is relevant for the development of the entire colony.

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Untersuchungen über die kernverhältnisse und morphologischen eigenschaften symbiontischer mikroorganismen bei verschiedenen insekten

Cited by 20 publications

References 114 publications

Tissue Localization of the Endosymbiotic Bacterium “ Candidatus Blochmannia floridanus” in Adults and Larvae of the Carpenter Ant Camponotus floridanus

Tissue Localization of the Endosymbiotic Bacterium “ Candidatus Blochmannia floridanus” in Adults and Larvae of the Carpenter Ant Camponotus floridanus

Systematic relationships and cospeciation of bacterial endosymbionts and their carpenter ant host species: proposal of the new taxon Candidatus Blochmannia gen. nov.

Relevance of the Endosymbiosis of Blochmannia floridanus and Carpenter Ants at Different Stages of the Life Cycle of the Host

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