Untersuchungen über den Einfluss des Cyclophosphamids auf Enzyme der Megakaryozyten

Abstract

By means of cytochemistry lactate dehydrogenase (LDH), alkaline and acid phosphatase (AP, SP) were identified in megakaryocytes of the rat. Even the smallest dosage of cyclophosphamide caused a decrease of LDH and AP activity, while the activity of SP increased. Simultaneously, the number of thrombocytes declined. Higher dosage corresponded to loss of LDH activity; in contrast, AP and SP remained unaltered. The reduction of LDH activity is suggested to be due to inhibition of enzyme synthesis caused by RNA inhibition. Increase of SP activity is interpreted as a morphological sign of enhanced metabolism or catabolism in the megakaryocytes