Untersuchungen �ber Plattenbau und Spiralbau, �ber Wachstum und Zerteilung der Paramylonk�rner

Pochmann, Alfred

doi:10.1007/bf01287835

Cited by 5 publications

(1 citation statement)

References 12 publications

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“…This is in contrast to the case of starch where the amylose chains are believed to be radial (Kreger, 1951). Our evidence for the tangential orientation of the glucan chains in paramylon confirms the deductions made by several authors from optical birefringence measurements (Kamptner, 1952;Pochman, 1956;Barras & Stone, 1969;Marchessault & Deslandes, 1979).…”

Section: Discussionsupporting

confidence: 90%

An electron diffraction study of paramylon storage granules from Euglena gracilis

Booy

Chanzy

Böudet

1981

Journal of Microscopy

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SUMMARY Paramylon storage granules from Euglena gracilis were characterized by electron diffraction techniques using electrons of various accelerating voltages: 2 MV for the thick granules and 100 kV for the thinner ones. Intact granules gave well resolved, characteristic (1→3)‐β‐d glucan fibre diffraction diagrams with the glucan molecular orientation parallel to the longer axis of the granule. Hydrated electron diffraction patterns with better resolution were obtained from thinner granules by examination at low temperatures of quench‐frozen specimens. In this case, the pattern indexed on an hexagonal system with a = b= 1·55 + 0·01 nm and c (fibre axis) = 1·86 ± 0·01 nm. Sections of embedded granules provided single crystal‐like electron diffraction patterns corresponding to various sections of the reciprocal lattice of (1→3)‐β‐d glucan (including the hko section). Finally, by scanning electron microscopy, it was shown that the granules swell on contact with water and take up a characteristic ribbed, pumpkin‐like shape.

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