Untersuchungen �ber den Chylomikronenstoffwechsel

Gries, F. A.; Jung, G.; Jahnke, K.

doi:10.1007/bf01484709

Cited by 4 publications

(1 citation statement)

References 32 publications

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“…Nitrite (xy 3 P ) concentration in the medium was determined by the Griess reaction [20]. Brie£y, 100 Wl of conditioned medium was mixed with an equal volume of Griess reagent [naphthylethylenediamine…”

Section: Nitrite Measurementmentioning

confidence: 99%

Inhibition of NADPH supply by 6‐aminonicotinamide: effect on glutathione, nitric oxide and superoxide in J774 cells

1998

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We have examined the integrity of J774 cell nitric oxide (NO) production and glutathione maintenance, whilst NADPH supply was compromised by inhibition of the pentose pathway with 6-aminonicotinamide. In resting cells 6-phosphogluconate accumulation began after 4 h and glutathione depletion after 24 h of 6-aminonicotinamide treatment. Cellular activation by lipopolysaccharide/interferon-V V decreased glutathione by V50% and synchronous 6-aminonicotinamide treatment exacerbated this to 31.2% of control (P 6 6 0.05). In activated cells xy 3 P production was inhibited by 60% with 6-aminonicotinamide (P 6 6 0.01), and superoxide production by 50% (P 6 6 0.01) in zymosan-activated cells. NADPH production via the pentose pathway is therefore important to sustain macrophage NO production whilst maintaining protective levels of glutathione.z 1998 Federation of European Biochemical Societies.

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Section: Nitrite Measurementmentioning

confidence: 99%