Untersuchungen an isolierten menschlichen Fettzellen verschiedener Fettgewebsregionen

Hj, Lisch; Sailer, S; Sandhofer, F; Braunsteiner, H

doi:10.1007/bf01485462

Cited by 9 publications

(4 citation statements)

References 11 publications

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“…In agreement with previous investigation [9,13,15,26], the present results show that the lipolysis of human subcutaneous adipose tissue is closely related to the size of the fat cells. Although the antilipolytic effect of insulin (100 gU/ml) seems to be cell sizedependent, this relationship may be more apparent than real.…”

Section: Discussionsupporting

confidence: 93%

Cell size and the antilipolytic effect of insulin in human subcutaneous adipose tissue

1975

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Lipolysis was studied in subcutaneous adipose tissue removed under local or general anaesthesia from subjects with marked difference in body weight and from obese subjects before and after intestinal shunt operations. The release of glycerol was measured in sections of adipose tissue incubated for two hours in bicarbonate buffer containing 4 per cent bovine albumin with no glucose added. The larger fat cells were more insulin sensitive than the smaller. A positive relationship was observed between the fat cell size and the antilipolytic effect of insulin (100 muU/ml). Insulin had no significant effect on the lipolytic effect of the catecholamines, when this is calculated as the increment in the glycerol release above the basal.

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Section: Discussionsupporting

confidence: 93%

Cell size and the antilipolytic effect of insulin in human subcutaneous adipose tissue

1975

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“…Glycerol was determined according to Kreutz [5] with some modifications [6]. The determination of the cell destruction index has also been described previously [6].…”

Section: Methodsmentioning

confidence: 99%

“…Immediately after the excision the adipose tissue was placed into KrebsRinger bicarbonate buffer [7] at body temperature. Approximately 20 rain after the removal of the adipose tissue, fat cells were isolated according to Rodbell [7] with some modification s [6]. In 3 cases (See Table 1, section A) the adipose tissue removed under general anaesthesia was divided into two equal portions, which were pre-incubated in a metabolic shaker for 30 min at room temperature in Krebs-Ringer bicarbonate buffer without or with procain hydrochloridc (10 mg/ml) respectively.…”

Section: Methodsmentioning

confidence: 99%

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Effect of general and local anaesthesia on basal and noradrenaline-stimulated lipolysis in isolated human subcutaneous fat cells

et al. 1974

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Summary. In isolated human fat cells of the subcutaneous abdominal region the effect of general anaesthesia as compared to local anaesthesia on basal and noradrenaline-stimulated lipolysis has been investigated. It could be shown that procain hydroehloride exerted a marked antilipolyCic action when added in vitro. Preincubation of the whole adipose tissue with procain hydrochloride prior to the isolation procedure did not alter the lipolytic activity in the fat cells. There was no significant difference in lipolysis between fat cells obtained from patients under general anaesthesia and cells from individuals under local anaesthesia with procain hydroehloride.

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Viewpoints in Obesity Research – a Critical Review

Sons¹

1985

Fette, Seifen, Anstrichm.

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This study describes aspects of obesity research, and especially the thinkingon theetiology, thedifferentiationofindividualformsofobesity,the problem of the size of fat cells, the assessment of obesity as a cardiovascular risk factor and the problem of the fatty infiltration of the liver in obesity. A summary of the literature on the subject is given. I n t r o d u c t i o n a n d D e f i n i t i o n Numerous definitions of obesity exist; let us take just one as representative: "Obesity is the propensity of the organism to excessive weight increase which occurs under certain conditions. It can lead to a clinical picture which includes a disturbance of the ratio of the body weight constituents in the sense of a more or less pronounced increase in the proportion of fat. Associated with this there may be physical and mental dsturbance of the organism"'.The cause of the obesity is always a disturbed energy balance, in that the intake of energy by the ingestion of food is greater than the energy consumption.The extent of the obesity is generally stated as a percentage overweight in relation to the normal weight of the person concerned. There are a number of systems of calculation and measurement relating to the boundaries of the normal weight range and hence also to the nosologically exact definition of the concept of obesity as a disease;as a rule, however, the normal body weight is calculated roughly in accordance with Broca's rule (body length in cm -100 = ideal weight in kg). If the normal weight according to Broca is exceeded by 20%, then obesity is present. However, the weight determined by using Broca's formula is related solely to the length of the body, and the peculiarities of the physique of the person concerned are not taken into account.In many cases the definition and quantification of obesity is also made on the basis of the ideal weight, with the ideal weight being designated as that at which, given a certain body length, the maximum life expectancy is anticipated in purely statistical terms'. As with the Brocu index,in theideal weight, which is m a d y based on an association between life expectancy and the ratio of body weight to body length, no account is taken of differences in the composition of the body" and constitution of the body4,". Doubts have also been expressed as to whether the data which are used as the basis for the calculations, and which were determined many years ago, are representative of the present population of the USA, and also especially of groups of the West European pop~lation"~~. On account of its ease of use the Broca index has held its own in clinical practice in spite of many disadvantages. Finally let us also mention that the terms adiposity, obesity, overweight and corpulency are used as synonymous designations for the disease. N u m b e r a n d s i z e of f a t c e l l s The questions of particular importance with regard to the etiology of obesity and also to the understanding of the results of therapy, are the number of fat cells deposited in theStandpunkte in der Erf...

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Untersuchungen an isolierten menschlichen Fettzellen verschiedener Fettgewebsregionen

Cited by 9 publications

References 11 publications

Cell size and the antilipolytic effect of insulin in human subcutaneous adipose tissue

Cell size and the antilipolytic effect of insulin in human subcutaneous adipose tissue

Effect of general and local anaesthesia on basal and noradrenaline-stimulated lipolysis in isolated human subcutaneous fat cells

Viewpoints in Obesity Research – a Critical Review

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