Untargeted phytochemical profiling and biological activity of small yellow onion (Allium flavum L.) from different regions of Romania

Moldovan, Cadmiel; Frumuzachi, Oleg; Babotă, Mihai; Pinela, José; Barros, Lillian; Rocchetti, Gabriele; López, Víctor; Lucini, Luigi; Crișan, Gianina; Mocan, Andrei

doi:10.1016/j.foodchem.2023.136503

Cited by 4 publications

(1 citation statement)

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“…The inhibitory potential of OpTh on the lipase enzyme, which plays a role in lipid breakdown metabolic processes, was evaluated using adapted in vitro tests for a microplate reader (Moldovan et al 2023). Each well contained 40 µL of the tested sample and 40 µL of type II lipase (L3126, Sigma-Aldrich, Saint Louis, MO, USA) from porcine pancreas (2.5 mg/mL prepared in Tris-Buffer (100 mM Tris-HCl and 5 mM CaCl2, pH 7.0)).…”

Section: Determination Of Lipase Inhibitory Activitymentioning

confidence: 99%

Exploring polyphenols extraction from Thuja occidentalis L. bark: Optimization, phytochemical profiling, and biological evaluation

Coman,

Babotă,

Nădășan

et al. 2023

BioRes

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Polyphenols were extracted from Thuja occidentalis L. bark and characterized in terms of phytochemical composition and biological activity. The optimization process investigated the impacts of extraction duration, ultrasound amplitude, and ethanol concentration on the total phenolic content in the extract. The antioxidant capacity was examined using DPPH and ABTS assays, and anti-enzyme activity against alpha-glucosidase, alpha-amylase, lipase, acetylcholinesterase, and tyrosinase was determined. The experimental model revealed optimal extraction parameters: a hydroethanolic solvent with 44% v:v ethanol, a 15-minute extraction time, and a 40% ultrasound amplitude. These parameters were validated and confirmed by reaching the target Total Phenolic Content (TPC) predicted by MODDE software. The resulting lyophilized extract exhibited a high polyphenolic content (161.84 ± 23.48 mg GAE/g dry extract) and demonstrated strong antioxidant properties. Notably, it showed significant inhibition of alpha-glucosidase and alpha-amylase, suggesting potential antidiabetic effects, though its inhibition of tyrosinase activity was relatively weak. These findings provide a foundation for further investigations to confirm the qualitative and quantitative presence of specific polyphenolic metabolites in the extract and elucidate the mechanisms underlying its bioactive properties.

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Section: Determination Of Lipase Inhibitory Activitymentioning

confidence: 99%