Untargeted Metabolomics Coupled with Chemometrics for Leaves and Stem Barks of Dioecious Morus alba L.

Wu, Chan; Wang, Huijun; Liu, Zhenying; Xu, Bo; Li, Zhuojun; Song, Pingping; Chao, Zhimao

doi:10.3390/metabo12020106

Cited by 11 publications

(6 citation statements)

References 71 publications

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“… 18 It has been reported that the untargeted metabolomics has been applied to analyze the change rules of metabolites in different stages, tissues, and processing modes of different plants. 19–21 These studies proved that metabolomics analysis could be an authentic tool for comparing active compounds from different organs of U. parvifolia .…”

Section: Introductionmentioning

confidence: 86%

Comparison analysis of metabolite profiling in seeds and bark of Ulmus parvifolia, a Chinese medicine species

Yin

Wang³

et al. 2022

Plant Signaling & Behavior

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Ulmus parvifolia ( U. parvifolia ) is a Chinese medicine plant whose bark and leaves are used in the treatment of some diseases such as inflammation, diarrhea and fever. However, metabolic signatures of seeds have not been studied. The seeds and bark of U. parvifolia collected at the seed ripening stage were used for metabolite profiling analysis through the untargeted metabolomics approach. A total of 2,578 and 2,207 metabolites, while 503 and 132 unique metabolites were identified in seeds and bark, respectively. Additionally, 574 differential metabolites (DEMs) were detected in the two different organs of U. parvifolia , which were grouped into 52 classes. Most kinds of metabolites classed into prenol lipids class. The relative content of flavonoids class was the highest. DEMs contained some bioactive compounds (e.g., flavonoids, terpene glycosides, triterpenoids, sesquiterpenoids) with antioxidant, anti-inflammatory, and anti-cancer activities. Most kinds of flavonoids and sesquiterpenes were up-regulated in seeds. There were more varieties of terpene glycosides and triterpenoids showing up-regulated in bark. The pathway enrichment was performed, while flavonoid biosynthesis, flavone and flavonol biosynthesis were worthy of attention. This study identified DEMs with pharmaceutical value between seeds and bark during seed maturation and offered a molecular basis for alternative or complementary use of seeds and bark of U. parvifolia as a Chinese medicinal material.

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Section: Introductionmentioning

confidence: 86%

Comparison analysis of metabolite profiling in seeds and bark of Ulmus parvifolia, a Chinese medicine species

Yin

Wang³

et al. 2022

Plant Signaling & Behavior

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“…PCA is an unsupervised recognition pattern, which transforms the original variable into a new set of independent variables by reducing the dimension of the data matrix. This independent variable is called principal components (PCs) [32] . All the analyses were performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Identification of volatile compounds and metabolic pathway during ultrasound-assisted kombucha fermentation by HS-SPME-GC/MS combined with metabolomic analysis

Wang

Ahmad

Zhu

et al. 2023

Ultrasonics Sonochemistry

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“…Subsequently, the algorithm of ANOVA p value and max fold change were used to filter. The specific filtration was as described by Wu et al [ 39 ]. As for the identification of compounds, it was carried out based on their mass spectral data using the Metlin database, relevant published literature, and mixed standard solution based on their retention times and fragmentation patterns.…”

Section: Methodsmentioning

confidence: 99%

A Comprehensive Quality Analysis of Different Colors of Medicinal and Edible Honeysuckle

Liu,

Cheng,

Chao

2023

Foods

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Honeysuckle (the dried flower bud or opening flower of Lonicera japonica Thunb.), a medicinal and edible substance, has is greatly popular among consumers for its remarkable health effects, such as antioxidant, antibacterial, and anti-inflammatory effects. However, due to the influences of processing methods, storage conditions, and other factors, honeysuckles show different colors which can directly reflect the quality and the price on the market. In order to comprehensively compare the quality of different colors, 55 batches of honeysuckle samples were collected and analyzed. Their color parameters, chlorophyll content (chl), total phenol content (TPC), total flavonoid content (TFC), antioxidant activity (AA), main active compounds, and metabolites were measured. As a result, the initial green-white (GW) samples, a kind of highest-quality honeysuckle, had the smallest a* value, largest h*, chl, TPC, TFC, and AA values, and highest content of chlorogenic acid and cynaroside. There was a significant difference between GW samples and a series of discolored samples. As the color darkened or lightened, the quality gradually decreased. The yellow-brown (YB) samples were of the worst quality and were no longer available for clinical and health purposes. A series of differential metabolites, such as quercetin-7-O-glucoside and secologanoside, could be used as important references to evaluate the quality of differently colored samples. The metabolic profile of honeysuckle provided new insights into the process of color change and laid a foundation for further honeysuckle quality control. The correlation results showed that the a* and h* values significantly affect the abovementioned quality indicators and the 10 main active compounds. In other words, the color difference could directly reflect the quality and clinical efficacy. Multiple regression analysis was carried out using combined L*, a*, and b* values to predict the quality of honeysuckle. This is the first time the quality of different color honeysuckle samples on the post-harvest link has been systematically compared and a demonstration of medicinal and edible substances with different colors has been provided.

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Untargeted Metabolomics Coupled with Chemometrics for Leaves and Stem Barks of Dioecious Morus alba L.

Cited by 11 publications

References 71 publications

Comparison analysis of metabolite profiling in seeds and bark of Ulmus parvifolia, a Chinese medicine species

Comparison analysis of metabolite profiling in seeds and bark of Ulmus parvifolia, a Chinese medicine species

Identification of volatile compounds and metabolic pathway during ultrasound-assisted kombucha fermentation by HS-SPME-GC/MS combined with metabolomic analysis

A Comprehensive Quality Analysis of Different Colors of Medicinal and Edible Honeysuckle

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