Untargeted Metabolomics by Using UHPLC–ESI–MS/MS of an Extract Obtained with Ethyl Lactate Green Solvent from Salvia rosmarinus

Abstract

Salvia rosmarinus (Lamiaceae), previously known as Rosmarinus officinalis, is a plant cultivated worldwide, native to the Mediterranean region. Its leaves are traditionally used for cooking. This species possesses numerous biological activities, including antioxidant, antimicrobial, anticancer, anti-inflammatory, and hepatoprotective properties. These biological properties are due to the presence of phenolic compounds, including rosmarinic acid and phenolic diterpenoids, such as carnosic acid and carnosol. In this study, we investigated the chemical composition of a green extract obtained by maceration with ethyl lactate for the first time. Seventy-five compounds were tentatively identified by UHPLC–ESI–MS/MS, including six organic acids, six cinnamic acid derivatives, five fatty acids, eighteen flavonoids, and thirty-eight terpenoids. Thus, abietane-type diterpenoids from the ethyl lactate extract were the predominant diterpenoids in the Chilean S. rosmarinus species, in contrast to the Chinese species, in which labdane and isopimarane-type diterpenoids were found for the first time. Finally, our study confirms that the extraction of S. rosmarinus with green ethyl lactate as a solvent is efficient and sustainable for the identification of flavonoids, phenols, and terpenoids from leaves.

Comparative Analysis of Metabolic Variations, Antioxidant Profiles and Antimicrobial Activity of Salvia hispanica (Chia) Seed, Sprout, Leaf, Flower, Root and Herb Extracts

“…The highest content of these compounds was found in flower extracts. The most abundant compound (peak 87 ) with quasimolecular ion at m/z 345.17046 (C 20 H 25 O 5 − ) has a unique fragmentation pattern with fragmentation ions at m/z 331.1508 and 315.1597 that have been previously observed for rosmadial and hydroxyrosmadial [ 46 ]. Therefore, this compound was assigned as a rosmadial derivative.…”

Phytochemical analysis for ten Peruvian Mentheae (Lamiaceae) by liquid chromatography associated with high resolution mass spectrometry

“…The LC-HRMS metabolite profile of the ethanolic extracts of the ten peruvian Mentheae was obtained in the negative mode (ESI (−)) and the detected compounds appear in Table 1 . Assignments were made based on the literature 21 – 37 . Isomers of quinic acid ( m/z 191.0556), danshensu ( m/z 197.0450), protocatechuic aldehyde ( m/z 137.0239), and caffeic acid ( m/z 179.0350) occur in most plants.…”

Enrichment of Olive Oils with Natural Bioactive Compounds from Aromatic and Medicinal Herbs: Phytochemical Analysis and Antioxidant Potential

“…Bold numbers represent the most abundant MS/MS fragment; LP: “Lianoelia Prevezas”; KA: “Konservoelia Artas”; LK: “Lianoelia Kerkyras” olive oils. * Castañeta et al, 2022 [ 6 ]. ** Geng et al, 2016 [ 7 ].…”