Untargeted metabolomics analysis reveals dynamic changes in co-fermentation with human milk-derived probiotics and Poria cocos

Wang, Qishan; Yang, Kai; Wei, Xinyue; Qiao, Weicang; Chen, Lijun

doi:10.3389/fmicb.2022.1032870

Cited by 5 publications

(3 citation statements)

References 52 publications

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“…Overall judgment cannot be made only based on the levels of one single substance. By comparing with the KEGG database, genes were classified according to their participation in pathways or functions, revealing the metabolic pathways involved in metabolite metabolism ( Xie et al, 2020 ; Wang et al, 2022 ). Thus, the impact of the biological metabolic process of the fermentation broth of the B1-28 and ΔFolA can be evaluated from a macroscopic point of view.…”

Section: Discussionmentioning

confidence: 99%

Effect of folA gene in human breast milk-derived Limosilactobacillus reuteri on its folate biosynthesis

Jiang,

Li,

Zhang

et al. 2024

Front. Microbiol.

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IntroductionFolate supplementation is crucial for the human body, and the chemically synthesized folic acid might have undesirable side effects. The use of molecular breeding methods to modify the genes related to the biosynthesis of folate by probiotics to increase folate production is currently a focus of research.MethodsIn this study, the folate-producing strain of Limosilactobacillus reuteri B1-28 was isolated from human breast milk, and the difference between B1-28 and folA gene deletion strain ΔFolA was investigated by phenotyping, in vitro probiotic evaluation, metabolism and transcriptome analysis.ResultsThe results showed that the folate producted by the ΔFolA was 2–3 folds that of the B1-28. Scanning electron microscope showed that ΔFolA had rougher surface, and the acid-producing capacity (p = 0.0008) and adhesion properties (p = 0.0096) were significantly enhanced than B1-28. Transcriptomic analysis revealed that differentially expressed genes were mainly involved in three pathways, among which the biosynthesis of ribosome and aminoacyl-tRNA occurred in the key metabolic pathways. Metabolomics analysis showed that folA affected 5 metabolic pathways, involving 89 different metabolites.DiscussionIn conclusion, the editing of a key gene of folA in folate biosynthesis pathway provides a feasible pathway to improve folate biosynthesis in breast milk-derived probiotics.

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Section: Discussionmentioning

confidence: 99%

Effect of folA gene in human breast milk-derived Limosilactobacillus reuteri on its folate biosynthesis

Jiang,

Li,

Zhang

et al. 2024

Front. Microbiol.

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“…As a tool of foodomics, metabolome including non-targeted and targeted metabolite analysis mainly studies the small molecule metabolites produced by organisms, and it possesses great potential for studying the dynamic changes of food components. The milk metabolomic of humans [ 14 ], cows [ 15 ] and sheep [ 16 ] has been reported and plenty of functional active ingredients were found.…”

Section: Introductionmentioning

confidence: 99%

The Study of Yak Colostrum Nutritional Content Based on Foodomics

Xiong

Pei

Bao

et al. 2023

Foods

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The utilization of yak milk is still in a primary stage, and the nutrition composition of yak colostrum is not systematically characterized at present. In this study, the lipids, fatty acids, amino acids and their derivatives, metabolites in yak colostrum, and mature milk were detected by the non−targeted lipidomics based on (ultra high performance liquid chromatography tandem quadrupole mass spectrometer) UHPLC−MS, the targeted metabolome based on gas chromatography−mass spectrometer (GC−MS), the targeted metabolome analysis based on UHPLC−MS, and the non-targeted metabolome based on ultra high performance liquid chromatography tandem quadrupole time of flight mass spectrometer (UHPLC−TOF−MS), respectively. Meanwhile, the nutrition composition of yak colostrum was compared with the data of cow mature milk in the literatures. The results showed that the nutritive value of yak colostrum was higher by contrast with yak and cow mature milk from the perspective of the fatty acid composition and the content of Σpolyunsaturated fatty acids (PUFAs), Σn−3PUFAs; the content of essential amino acid (EAA) and the ratio of EAA/total amino acid (TAA) in yak colostrum were higher than the value in yak mature milk; and the content of functional active lipids including phosphatidylcholines (PC), phosphatidylglycerol (PG), phosphatidylserine (PS), lyso-phosphatidylcholine (LPC), lyso-phosphatidylglycerol (LPG), lyso-phosphatidylinositol (LPI), sphingomyelin (SM), ganglioside M3 (GM3), ganglioside T3 (GT3), and hexaglycosylceramide (Hex1Cer) in yak colostrum, was higher than the value of yak mature milk. Moreover, the differences of nutritive value between yak colostrum and mature milk were generated by the fat, amino acids and carbohydrate metabolism that were regulated by the ovarian hormone and referencesrenin-angiotensin-aldosterone system in yaks. These research results can provide a theoretical basis for the commercial product development of yak colostrum.

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“…Pachymic acid (PA) is one of the main active ingredients extracted from medicinal fungus Poria cocos 8 . Pachymic acid has been reported with multiple pharmacological effects, including neuroprotection 9 , anti-inflammatory 10 , anti-ischemia/reperfusion effects 11 , and anticancer action 12 .…”

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confidence: 99%

RNA-Seq Analysis Reveals Potential Neuroprotective Mechanisms of Pachymic Acid Toward Iron-Induced Oxidative Stress and Cell Death

Hu,

Yang,

et al. 2024

Cell Transplant

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Iron dysregulation is a crucial factor in the development of neurological diseases, leading to the accumulation of reactive oxygen species (ROS) and oxidative stress, triggering inflammatory responses, and ultimately causing neurological impairment. Pachymic acid (PA) is an active ingredient extracted from the medicinal fungus Poria cocos, which has been reported with multiple pharmacological effects, including anti-inflammatory, anti-ischemia/reperfusion, and anticancer actions. In this study, we test whether PA have neuroprotection effect aganist ferrous ions induced toxicity in SH-SY5Y cells. It was found that pre-treatment with PA reduced intracellular ROS levels, increased mitochondrial membrane potential, and protected cells from apoptotic death. RNA-seq and qRT-PCR results indicated that PA can regulate the key genes IL1B, CXCL8, CCL7, and LRP1 on the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, such as NF-κB signaling pathway, IL-17 signaling pathway, to prevent Fe2+-induced apoptotic cell death. Our research indicated that PA has potential therapeutic effects on the neuroprotection by regulating neuroinflammation and oxidative stress damage.

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Untargeted metabolomics analysis reveals dynamic changes in co-fermentation with human milk-derived probiotics and Poria cocos

Cited by 5 publications

References 52 publications

Effect of folA gene in human breast milk-derived Limosilactobacillus reuteri on its folate biosynthesis

Effect of folA gene in human breast milk-derived Limosilactobacillus reuteri on its folate biosynthesis

The Study of Yak Colostrum Nutritional Content Based on Foodomics

RNA-Seq Analysis Reveals Potential Neuroprotective Mechanisms of Pachymic Acid Toward Iron-Induced Oxidative Stress and Cell Death

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