Untargeted metabolomic analysis using UPLC-MS/MS identifies metabolites involved in shoot growth and development in pruned tea plants (Camellia sinensis (L.) O. Kuntz)

Arkorful, Emmanuel; Yu, Ying; Chen, Changsong; Li, Lu; Hu, Shunkai; Yu, Hanpu; Ma, Qingping; Thangaraj, Kuberan; Periakaruppan, Rajiv; Chen, Xuan; Li, Xinghui

doi:10.1016/j.scienta.2019.109164

Cited by 16 publications

(14 citation statements)

References 31 publications

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“…[5,6] Therefore, in recent years, more and more scientists have applied it to the field of tea studies to obtain effective secondary metabolism rules of tea plants and different metabolites among different tea species, so as to deepen the understanding of endogenous information of tea. [7,8] QDA is not only a flavor inspection method with the function of analysis, description and quantification, but also a practical quality evaluation tool. [9][10][11] This method has also been gradually applied to the field of tea sensory evaluation.…”

Section: Introductionmentioning

confidence: 99%

Identification of volatile components and analysis of aroma characteristics of Jiangxi Congou black tea

Yue

Yang

Qin

et al. 2020

International Journal of Food Properties

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Section: Introductionmentioning

confidence: 99%

Identification of volatile components and analysis of aroma characteristics of Jiangxi Congou black tea

Yue

Yang

Qin

et al. 2020

International Journal of Food Properties

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“…Furthermore, 80 differential metabolites were annotated in new shoots of pruned tea, among which were the auxin-associated metabolites indole-3-acetonitrile and menaquinone, which accounted for the increased levels of indole-3acetic acid in shoots and the subsequently increased shoot growth. 8 Plant growth depends on the presence and availability of water and nutrient in the soil and the ability of plant roots to absorb available nutrients from the soil. Plants absorb nutrients from the growth medium and distribute to other parts of the plants.…”

Section: ■ Discussionmentioning

confidence: 99%

“…In our previous study, pruning significantly increased the number and weight of new shoots due to the significant increase in the synthesis of indole-3-acetic acid in shoots of pruned tea. Furthermore, 80 differential metabolites were annotated in new shoots of pruned tea, among which were the auxin-associated metabolites indole-3-acetonitrile and menaquinone, which accounted for the increased levels of indole-3-acetic acid in shoots and the subsequently increased shoot growth . Plant growth depends on the presence and availability of water and nutrient in the soil and the ability of plant roots to absorb available nutrients from the soil.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Analyses Provide New Insights into Signaling Mechanisms for Nutrient Uptake by Lateral Roots of Pruned Tea Plant (Camellia sinensis)

Arkorful

Zou

et al. 2020

J. Agric. Food Chem.

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Pruning is an important plant management practice in tea cultivation. However, the mechanism underlying the dynamics of nutrient uptake by roots of pruned tea is unknown. This study investigated the metabolic alterations in lateral roots of pruned tea to unveil the mechanism of nutrient uptake. Elemental analysis revealed that pruning significantly increases the uptake of nutrients by lateral roots. Metabolic profiling showed significant metabolic variations in lateral roots of pruned tea. Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis revealed that flavonoid biosynthesis, phenylpropanoid biosynthesis, and amino acid metabolism were differentially regulated in lateral roots. Caffeine metabolism was significantly hindered, while ethylene signaling was significantly induced in lateral roots of pruned plants. In addition, intermediates in the tricarboxylic acid (TCA) cycle were upregulated, indicating high rates of the TCA cycle. Therefore, pathways related to phenylpropanoid biosynthesis, TCA cycle, ethylene biosynthesis, and metabolism of amino acids contribute to higher nutrient uptake by lateral roots of the tea plant.

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“…Several studies have reported the effects of pruning on tea quality and constituents. For example, the synthesis of indole-3-acetonitrile and menaquinone increased in pruned tea plants and enhanced shoot growth and development [17]. Similarly, EGCG concentration was much higher in pruned than in unpruned tea trees, and serine carboxypeptidase was upregulated for biosynthesis of EGCG in pruned tea plants [19].…”

Section: Introductionmentioning

confidence: 99%

“…Management practices adopted by farmers are most important for plant growth and formation of high-quality constituents. Under different agronomic practices, such as the application of organic fertilizers, shade treatment, pruning or non-pruning of tea trees, different metabolite compositions will be formed based on metabolite contents [17,18]. Pruning is an essential, and the most significant, agronomic management practice, aimed to enhance branching and increase the number of tender leaves.…”

Section: Introductionmentioning

confidence: 99%

Effects of Long-Term Non-Pruning on Main Quality Constituents in ‘Dancong’ Tea (Camellia sinensis) Leaves Based on Proteomics and Metabolomics Analysis

Chen

Zhou

et al. 2021

Foods

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‘Dancong’ tea is a famous traditional Oolong tea. In order to keep the original taste of “ancient tea trees”, most of the ‘Dancong’ tea plants are planted in a single plant pattern without pruning. The objective of this study was to explore the effects of long-term non-pruning on main quality constituents in ‘Dancong’ tea. The results showed that the contents of free amino acids, chlorophylls, and floral-honey aromatic substances in tea leaves of unpruned tea plants were higher than those in every year pruned tea plants, while the catechin content in leaves of pruned tea plants was higher than that in leaves of unpruned tea plants. Quantitative proteomics analysis showed that most enzymes involved in biosynthesis of catechins were downregulated in leaves of unpruned tea plants. Five proteins involved in chlorophyll metabolism and 12 proteins related to photosynthesis were upregulated, and the results suggested that higher chlorophyll content and more efficient photosynthetic energy conversion may be important for the higher accumulation of special quality components in leaves of unpruned tea plants. The findings of this study will advance our understanding of the mechanism of formation of different metabolites in leaves of unpruned and pruned tea plants.

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Untargeted metabolomic analysis using UPLC-MS/MS identifies metabolites involved in shoot growth and development in pruned tea plants (Camellia sinensis (L.) O. Kuntz)

Cited by 16 publications

References 31 publications

Identification of volatile components and analysis of aroma characteristics of Jiangxi Congou black tea

Identification of volatile components and analysis of aroma characteristics of Jiangxi Congou black tea

Metabolomic Analyses Provide New Insights into Signaling Mechanisms for Nutrient Uptake by Lateral Roots of Pruned Tea Plant (Camellia sinensis)

Effects of Long-Term Non-Pruning on Main Quality Constituents in ‘Dancong’ Tea (Camellia sinensis) Leaves Based on Proteomics and Metabolomics Analysis

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