Untargeted metabolic profiling reveals potential biomarkers in myocardial infarction and its application

Abstract

Although some important biomarkers for myocardial injury have been identified, there still lacks a systematic view of the development and progression of myocardial infarction, including enzymatic regulation, metabolite levels, fluxes, etc., which are pivotal to elucidate the physiological mechanism of disease. Here we present an untargeted analytical approach based on gas chromatography coupled with mass spectrometry (GC-MS) to map the temporal metabolic profilings in blood sera of myocardial infarction rat model prepared by left coronary artery ligation. Using XCMS software (http://metlin.scripps.edu/download/), data processing was simplified greatly. We identified the changes in circulating levels of 24 metabolites during the myocardial ischemia. By combination of previous proteomic results, it gives rise to a new insight view of energy metabolism changes referring to anaerobic glycolysis, citric acid cycle, fatty acid beta-oxidation, and some amino acids metabolism. With these altered metabolism pathways as possible drug targets, we validated a role for the presented metabonomic profiling in the systematic understanding of the action mechanism of component-complex medicine herbs, such as Radix Ophiopogonis, a widely-used anti-myocardial ischemia herbal medicine in Asia.

Metabolomic signature of arterial stiffness in male patients with peripheral arterial disease

“…Our findings revealed that AngSc is correlated with serum levels of phenylalanine and tyrosine. Accordingly, the results of animal experiments 28 and human studies 29 indicate that myocardial infarction is associated with increased levels of phenylalanine. It could be hypothesized that the association of phenylalanine with the extent of atherosclerosis is influenced by high-grade oxidative stress.…”

Biomarkers in the early period of acute myocardial infarction in rat serum and protective effects of Shexiang Baoxin Pill using a metabolomic method

“…Though some biomarkers related to AMI have been identified using a metabolomics method in recent years (Zhang et al, 2009;Lv et al, 2010;Yao et al, 2010), the metabolites that changed in the early period of AMI had still not been investigated. To achieve a complete understanding of the metabolite profile associated with AMI in the early period, an AMI model using the left anterior descending coronary artery (LADCA) ligation method was adopted, and then the serum biomarkers were analyzed after 5 h of the heart operation using a metabolomic method.…”

LC/MS-based polar metabolite profiling reveals gender differences in serum from patients with myocardial infarction

“…Interestingly, variation of BCAAs was strongly correlated with underlying disease such as diabetes and therapeutic intervention for CVD. For MI specific studies, rat models induced by coronary artery ligation or isoproterenol have been used to identify the metabolic alterations and clarify the pathophysiological mechanisms in MI [14][15][16]. However, few clinical models have been used in metabolomics approaches for MI.…”