Untargeted Fecal Metabolomic Analyses across an Industrialization Gradient Reveal Shared Metabolites and Impact of Industrialization on Fecal Microbiome-Metabolome Interactions

Haffner, Jacob J.; Katemauswa, Mitchelle; Kagoné, Thérèse; Hossain, Ekram; Jacobson, David P.; Flores, Karina; Parab, Adwaita R.; Obregón-Tito, Alexandra J.; Tito, Raúl Y.; Reyes, Luis Marin; Troncoso-Corzo, Luzmila; Guija-Poma, Emilio; Méda, Nicolas; Carabin, Hélène; Honap, Tanvi P.; Sankaranarayanan, Krithivasan; Lewis, Cecil M.; McCall, Laura-Isobel

doi:10.1128/msystems.00710-22

Cited by 4 publications

(2 citation statements)

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“…Ultra‐deep sequencing of Hadza hunter‐gatherers from Tanzania has identified many new microbial species and allowed for an in‐depth analysis of taxa associated with industrialised populations, and taxa that are under‐represented or lost relative to traditional societies (Carter et al., 2023 ). Analysis of the functional capacity encoded in the sequenced genomes also revealed differences (Table 1 ), which is broadly in agreement with a recent faecal metabolome study across several societies that demonstrated differences across an industrialisation gradient for some metabolites (for example amino acid‐conjugated bile acids, Haffner et al., 2022 ).…”

Section: The Gut Microbiota Fibre and Health In Different Populationssupporting

confidence: 86%

Dietary fibre optimisation in support of global health

Ramsteijn,

Louis

2024

Microbial Biotechnology

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The human gut microbiota influences its host via multiple molecular pathways, including immune system interactions, the provision of nutrients and regulation of host physiology. Dietary fibre plays a crucial role in maintaining a healthy microbiota as its primary nutrient and energy source. Industrialisation has led to a massive decrease of habitual fibre intake in recent times, and fibre intakes across the world are below the national recommendations. This goes hand in hand with other factors in industrialised societies that may negatively affect the gut microbiota, such as medication and increased hygiene. Non‐communicable diseases are on the rise in urbanised societies and the optimisation of dietary fibre intake can help to improve global health and prevent disease. Early life interventions shape the developing microbiota to counteract malnutrition, both in the context of industrialised nations with an overabundance of cheap, highly processed foods, as well as in Low‐ and Middle‐Income Countries (LMICs). Adequate fibre intake should, however, be maintained across the life course to promote health. Here we will discuss the current state of dietary fibre research in the global context and consider different intervention approaches.

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Section: The Gut Microbiota Fibre and Health In Different Populationssupporting

confidence: 86%

Dietary fibre optimisation in support of global health

Ramsteijn,

Louis

2024

Microbial Biotechnology

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“…Regarding human stool samples, which reflect the gut metabolome, most of the published research has focused on characterizing its complex bacterial composition using next-generation microbial DNA sequencing and sophisticated metagenomic techniques. However, a growing number of microbiome researchers are recognizing that considerable information could be gained by using a more integrative approach that also includes comprehensive fecal metabolite analysis ( Karu et al, 2018 ; Haffner et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

The gut metabolome in a cohort of pregnant and lactating women from Antioquia-Colombia

Londoño-Osorio,

Leon-Carreño,

Cala

et al. 2024

Front. Mol. Biosci.

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Nutrition during the perinatal period is an essential component of health and one that can severely impact the correct development of a human being and its overall condition, in all the subsequent stages of life. The availability of several compounds, mainly macronutrients and micronutrients, plays a key role in the balanced nutrition of both mother and baby and is a process with direct relation to the gut microbiome. Thus, we hereby refer to the set of small molecules derived from gut microbiome metabolism as the gut metabolome. These continuous processes occurring in the gut of a gestating or lactating mother related to microbial communities and nutrients, can be revealed by metabolomics. In this study, we explore for the first time the gut metabolome of pregnant and lactating women, from our region of Antioquia-Colombia, applying untargeted metabolomics by LC-QTOF-MS, and molecular networking. Regarding the gut metabolome composition of the cohort, we found, key metabolites that can be used as biomarkers of microbiome function, overall metabolic health, dietary intake, pharmacology, and lifestyle. In our cohort, pregnant women evidenced a significantly higher abundance of prostaglandins, alkaloids, corticosteroids, organosilicons, and natural toxins, while in lactating women, lipids stand out. Our results suggest that unveiling the metabolic phenotype of the gut microbiome of an individual, by untargeted metabolomics, allows a broad visualization of the chemical space present in this important niche and enables the recognition of influential indicators of the host’s health status and habits, especially of women during this significant perinatal period. This study constitutes the first evidence of the use of untargeted LC-QTOF-MS coupled with molecular networking analysis, of the gut microbiome in a Colombian cohort and establishes a methodology for finding relative abundances of key metabolites, with potential use in nutritional and physiological state assessments, for future personalized health and nutrition practices.

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