Untargeted and targeted metabolomics analyses of blackberries – Understanding postharvest red drupelet disorder

Kim, Moo Jung; Lee, Mee Youn; Shon, Jong Cheol; Kwon, Yong Sung; Liu, Kwang-Hyeon; Lee, Choong Hwan; Ku, Kang‐Mo

doi:10.1016/j.foodchem.2019.125169

Cited by 37 publications

(27 citation statements)

References 23 publications

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“…The rapid accumulation of LPC and LPE has been reported in many plant species that incur by stress ( Narvaez-Vasquez et al, 1999 ). Additionally, in research on blackberry red drupelet disorder research, phospholipids were a major constituent of cell membranes and the increased LPC level in red drupelet disorder indicated a loss of cell membrane integrity ( Kim et al, 2019 ). Therefore, a higher LPC content may be an important biomarker for cold damage to plants.…”

Section: Discussionmentioning

confidence: 99%

Comparative Metabolomic and Transcriptomic Studies Reveal Key Metabolism Pathways Contributing to Freezing Tolerance Under Cold Stress in Kiwifruit

et al. 2021

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Cold stress poses a serious treat to cultivated kiwifruit since this plant generally has a weak ability to tolerate freezing tolerance temperatures. Surprisingly, however, the underlying mechanism of kiwifruit’s freezing tolerance remains largely unexplored and unknown, especially regarding the key pathways involved in conferring this key tolerance trait. Here, we studied the metabolome and transcriptome profiles of the freezing-tolerant genotype KL (Actinidia arguta) and freezing-sensitive genotype RB (A. arguta), to identify the main pathways and important metabolites related to their freezing tolerance. A total of 565 metabolites were detected by a wide-targeting metabolomics method. Under (−25°C) cold stress, KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway annotations showed that the flavonoid metabolic pathways were specifically upregulated in KL, which increased its ability to scavenge for reactive oxygen species (ROS). The transcriptome changes identified in KL were accompanied by the specific upregulation of a codeinone reductase gene, a chalcone isomerase gene, and an anthocyanin 5-aromatic acyltransferase gene. Nucleotides metabolism and phenolic acids metabolism pathways were specifically upregulated in RB, which indicated that RB had a higher energy metabolism and weaker dormancy ability. Since the LPCs (LysoPC), LPEs (LysoPE) and free fatty acids were accumulated simultaneously in both genotypes, these could serve as biomarkers of cold-induced frost damages. These key metabolism components evidently participated in the regulation of freezing tolerance of both kiwifruit genotypes. In conclusion, the results of this study demonstrated the inherent differences in the composition and activity of metabolites between KL and RB under cold stress conditions.

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Section: Discussionmentioning

confidence: 99%

Comparative Metabolomic and Transcriptomic Studies Reveal Key Metabolism Pathways Contributing to Freezing Tolerance Under Cold Stress in Kiwifruit

et al. 2021

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“…Previous studies have investigated physiochemical changes in reverted drupelets and have found that the anthocyanin content is significantly lower in reverted drupelets compared with fully black drupelets (Edgley et al, 2019a;Kim et al, 2019). Kim et al (2019) harvested berries from 'Apache', 'Ouachita', and 'Triple Crown' and observed a 39.1% to 43.4% decrease in total anthocyanin content in red drupelets after a week in cold storage. A 41.7% average decrease in cyanidin-3-glucoside, the dominant anthocyanin present in blackberries, was also found in all three cultivars (Kim et al, 2019).…”

mentioning

confidence: 99%

Effect of Harvest Time and Fruit Firmness on Red Drupelet Reversion in Blackberry

Armour¹,

Worthington²,

Clark³

et al. 2021

horts

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Red drupelet reversion (RDR) is a postharvest disorder of blackberries (Rubus L. subgenus Rubus Watson) in which fully black drupelets revert to red after harvest. This disorder can negatively impact consumer perception of fresh-market blackberries. The cause of RDR is hypothesized to be related to intracellular damage sustained because of mechanical and environmental stress during and after harvest. Cultivars differ in susceptibility to this disorder; and cultural factors, including nitrogen rate, harvest and shipping practices, and climate during harvest, influence RDR severity. In this 2-year study, seven genotypes (cultivars and advanced selections) developed in the University of Arkansas System Division of Agriculture (UA) blackberry breeding program, with a range of fruit textures, were evaluated to determine whether firmness was correlated with RDR. In addition, fruit was harvested at four different times (7:00 am, 10:00 am, 1:00 pm, and 4:00 pm) to investigate whether harvest time influences RDR. All seven genotypes were harvested at the four times on two harvest dates per year and evaluated for RDR and firmness after 1 week of cold storage (5 °C). Fruit harvested early in the day had less RDR, with 7:00 am harvests having the least RDR in both years. Significant genotypic differences in RDR and fruit firmness were found in each year. Firmness was negatively correlated with RDR in 2018 and 2019. These results indicate that growers may be able to reduce the prevalence of RDR by choosing cultivars with firm fruit texture and harvesting early in the day.

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“…For the metabolic pathway analysis, the original data including all the metabolites of samples was used. The pathway analysis was performed with MetaboAnalyst (www.metaboanalyst.ca/) [38,39].…”

Section: Discussionmentioning

confidence: 99%

1H-NMR Metabolomics Analysis of the Effect of Rubusoside on Serum Metabolites of Golden Hamsters on a High-Fat Diet

Jiang

et al. 2020

Molecules

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Rubusoside is a natural sweetener and the active component of Rubus suavissimus. The preventive and therapeutic effect of rubusoside on high-fat diet-induced (HFD) serum metabolite changes in golden hamsters was analyzed by 1H-NMR metabolomics to explore the underlying mechanism of lipid metabolism regulation. 1H-NMR serum metabolomics analyses revealed a disturbed amino acid-, sugar-, fat-, and energy metabolism in HFD animals. Animals supplemented with rubusoside can partly reverse the metabolism disorders induced by high-fat diet and exerted good anti-hypertriglyceridemia effect by intervening in some major metabolic pathways, involving amino acid metabolism, synthesis of ketone bodies, as well as choline and 4-hydroxyphenylacetate metabolism. This study indicates that rubusoside can interfere with and normalize high-fat diet-induced metabolic changes in serum and could provide a theoretical basis to establish rubusoside as a potentially therapeutic tool able to revert or prevent lipid metabolism disorders.

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Untargeted and targeted metabolomics analyses of blackberries – Understanding postharvest red drupelet disorder

Cited by 37 publications

References 23 publications

Comparative Metabolomic and Transcriptomic Studies Reveal Key Metabolism Pathways Contributing to Freezing Tolerance Under Cold Stress in Kiwifruit

Comparative Metabolomic and Transcriptomic Studies Reveal Key Metabolism Pathways Contributing to Freezing Tolerance Under Cold Stress in Kiwifruit

Effect of Harvest Time and Fruit Firmness on Red Drupelet Reversion in Blackberry

1H-NMR Metabolomics Analysis of the Effect of Rubusoside on Serum Metabolites of Golden Hamsters on a High-Fat Diet

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