Untargeted 1H-NMR Urine Metabolomic Analysis of Preterm Infants with Neonatal Sepsis

Georgiopoulou, Panagiota D.; Chasapi, Styliani A.; Irene, Christopoulou; Varvarigou, Anastasia; Spyroulias, Georgios A.

doi:10.3390/app12041932

Cited by 6 publications

(7 citation statements)

References 35 publications

(43 reference statements)

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“…Urine sample preparation as well as NMR spectra acquisition and processing were conducted following the standard metabolomic procedures and methodology as reported by Georgakopoulou et al, 2020, and Georgiopoulou et al, 2022 [ 28 , 29 ].…”

Section: Sample Preparation and Nmr Analysis Methodologymentioning

confidence: 99%

Identification of Novel Biomarkers in Late Preterm Neonates with Respiratory Distress Syndrome (RDS) Using Urinary Metabolomic Analysis

et al. 2023

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Urine metabolomics is gaining traction as a means of identifying metabolic signatures associated with health and disease states. Thirty-one (31) late preterm (LP) neonates admitted to the neonatal intensive care unit (NICU) and 23 age-matched healthy LPs admitted to the maternity ward of a tertiary hospital were included in the study. Proton nuclear magnetic resonance (1H NMR) spectroscopy was employed for urine metabolomic analysis on the 1st and 3rd days of life of the neonates. The data were analyzed using univariate and multivariate statistical analysis. A unique metabolic pattern of enhanced metabolites was identified in the NICU-admitted LPs from the 1st day of life. Metabolic profiles were distinct in LPs presenting with respiratory distress syndrome (RDS). The discrepancies likely reflect differences in the gut microbiota, either due to variations in nutrient intake or as a result of medical interventions, such as the administration of antibiotics and other medications. Altered metabolites could potentially serve as biomarkers for identifying critically ill LP neonates or those at high risk for adverse outcomes later in life, including metabolic risks. The discovery of novel biomarkers may uncover potential targets for drug discovery and optimal periods for effective intervention, offering a personalized approach.

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Section: Sample Preparation and Nmr Analysis Methodologymentioning

confidence: 99%

Identification of Novel Biomarkers in Late Preterm Neonates with Respiratory Distress Syndrome (RDS) Using Urinary Metabolomic Analysis

et al. 2023

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“…For example, clinical tests based on NMR analysis of biomarkers in urine have led to the accurate diagnosis of a number of diseases, including pediatric nephropathies and neonatal sepsis in preterm infants. [38][39][40][41][42][43] Therefore, benchtop NMR demonstrates great potential to be a rapid screening tool in clinical settings, which further advocates for its routine application.…”

Section: Prospects Of Benchtop Nmr As a Profiling And Discovery Toolmentioning

confidence: 99%

Slice through the water—Exploring the fundamental challenge of water suppression for benchtop NMR systems

Pellizzari,

Soong,

Downey

et al. 2024

Magnetic Reson in Chemistry

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Benchtop NMR provides improved accessibility in terms of cost, space, and technical expertise. In turn, this encourages new users into the field of NMR spectroscopy. Unfortunately, many interesting samples in education and research, from beer to whole blood, contain significant amounts of water that require suppression in 1H NMR in order to recover sample information. However, due to the significant reduction in chemical shift dispersion in benchtop NMR systems, the sample signals are much closer to the water resonance compared to those in a corresponding high‐field NMR spectrum. Therefore, simply translating solvent suppression experiments intended for high‐field NMR instruments to benchtop NMR systems without careful consideration can be problematic. In this study, the effectiveness of several popular water suppression schemes was evaluated for benchtop NMR applications. Emphasis is placed on pulse sequences with no, or few, adjustable parameters making them easy to implement. These fall into two main categories: (1) those based on Pre‐SAT including Pre‐SAT, PURGE, NOESY‐PR, and g‐NOESY‐PR and (2) those based on binomial inversion including JRS and W5‐WATERGATE. Among these schemes, solvent suppression sequences based on Pre‐SAT offer a general approach for easy solvent suppression for samples with higher analyte concentrations (sucrose standard and Redbull™). However, for human urine, binomial‐like sequences were required. In summary, it is demonstrated that highly efficient water suppression approaches can be implemented on benchtop NMR systems in a simple manner, despite the limited spectral dispersion, further illustrating the potential for widespread implementation of these approaches in education and research.

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“…The paper by Georgiopoulou et al [6] is the last research article related to biomedical applications of NMR metabolomics included in our issue. It proposes an analysis of urine samples of preterm infants with neonatal sepsis, a systemic infection difficult to diagnose in its early stages and thus reporting high rates of morbidity and mortality.…”

Section: Nmr-based Metabolomicsmentioning

confidence: 99%

Applications and Challenges for Metabolomics via Nuclear Magnetic Resonance Spectroscopy

Vignoli

Tenori

2022

Applied Sciences

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Untargeted 1H-NMR Urine Metabolomic Analysis of Preterm Infants with Neonatal Sepsis

Cited by 6 publications

References 35 publications

Identification of Novel Biomarkers in Late Preterm Neonates with Respiratory Distress Syndrome (RDS) Using Urinary Metabolomic Analysis

Identification of Novel Biomarkers in Late Preterm Neonates with Respiratory Distress Syndrome (RDS) Using Urinary Metabolomic Analysis

Slice through the water—Exploring the fundamental challenge of water suppression for benchtop NMR systems

Applications and Challenges for Metabolomics via Nuclear Magnetic Resonance Spectroscopy

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