Untangling the Rhizosphere Bacterial Community Composition and Response of Soil Physiochemical Properties to Different Nitrogen Applications in Sugarcane Field

Abstract

Minimizing the use of chemical fertilizers and investigating an appropriate ecofriendly level of nitrogen fertilizer is the key to sustainable agriculture. Sugarcane is the main cash crop of China, especially in the Guangxi region. Information regarding the effect of different nitrogen levels on sugarcane rhizosphere microbiota is still limited. In this study, we evaluated the effect of four different levels of nitrogen fertilizers on rhizosphere bacterial composition using high throughput sequencing, along with soil physiochemical properties, sugarcane agronomic and yield performance. The four treatment combinations were CK (no fertilizers), L (Low, 100 kg ha–1), M (Medium, 150 kg ha–1), and H (High, 200 kg ha–1). The results showed that M nitrogen application significantly altered the rhizosphere bacterial community, soil properties, and sugarcane yield. The richness and evenness of the bacterial community were higher in M treatment than CK. In M treatment important bacterial phyla Acidobacteria and Proteobacteria increased by 47 and 71%, respectively; and at genus level, Acidothermus and Bradyrhizobium increased by 77.2 and 30.3%, respectively, compared to CK. Principal component analysis (PCA) and cluster analysis further confirmed the level of differences among the treatments. The PCA analysis explained 80% of the total variation among the treatments. Spearmen correlation heatmap showed that environmental factors such as pH, AP (available phosphorous), AK (available potassium), and SCAT (soil catalase) were the key factors impacting sugarcane rhizosphere microbiome composition. The H and L nitrogen application alter the bacterial community and sugarcane performance but the M nitrogen application appears to be ecofriendly, productive, and an appropriate nitrogen application rate that could be further used in the Guangxi region.

Biochar and Manure Applications Differentially Altered the Class 1 Integrons, Antimicrobial Resistance, and Gene Cassettes Diversity in Paddy Soils

“…HGT facilitates bacterial adaptive behavior and rapid evolution and promotes antimicrobial resistance genes (ARGs) across bacteria species in the environment ( Martínez, 2008 ; Zhu et al, 2017 ; Ali et al, 2020a ). Mobile genetic elements (MGEs) mediate the HGT, which could enhance the lateral gene flow among the clinical isolates ( Stecher et al, 2012 ; Chen et al, 2016 ; An et al, 2018 ; Khan et al, 2022 ). Thus, understanding the HGT is critical for resolving health issues caused by antimicrobial resistance and their subsequent dissemination in the environment.…”

Influence of Biochar on Soil Nutrients and Associated Rhizobacterial Communities of Mountainous Apple Trees in Northern Loess Plateau China

“…In the case of the most abundant genera, Sphingomonas , Lachnospiracea , RB41 , Ellin6055 , Kineosporia , Massilia , and Bacillus were recorded in the biochar-applied treatments, in which the Sphingomonas genus belongs to the phylum Proteobacteria and plays a beneficial role in the availability of nitrogen to plants [ 80 ]. Huang et al [ 35 ] argued that the Lachnospiracea genera in soil was positively associated with soil pH, which can be attributed to biochar application in our study.…”

“…Soil physiochemical properties largely influence soil bacterial abundance and composition [ 80 , 89 , 90 ]. In this study, biochar application significantly improved soil nutrients ( Table 3 ).…”

Dynamics of rhizosphere bacterial communities and soil physiochemical properties in response to consecutive ratooning of sugarcane

“…(5-ACGTCATCCCCACCTTCC-3), V5-V7 region of 16S rRNA gene was amplified (Khan et al, 2022a). The PCR amplification was conducted using a 25-µL reaction mixture that contained 20 ng of DNA template, 0.5 µL of dNTP, 10 µL of KOD (kodakaraensis) polymerase buffer, 0.25 µL of DNA polymerase, 5 µL of high GC enhancer, and 1.0 µL of each primer (Pang et al, 2019).…”