Untangling metabolic and communication networks: interactions of enterics with phytobacteria and their implications in produce safety

Teplitski, Max; Warriner, Keith; Bartz, Jerry A.; Schneider, Keith R.

doi:10.1016/j.tim.2010.11.007

Cited by 77 publications

(67 citation statements)

References 63 publications

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“…In addition to microorganisms with symbiotic or parasitic plant-microbe interactions, the endosphere can be a reservoir for potentially human pathogenic bacteria such as human enteric pathogens (7,12). Recent outbreaks highlight important deficiencies in our understanding of the ecology of enteric pathogens outside of their human and animal hosts (43).…”

mentioning

confidence: 99%

Banana-Associated Microbial Communities in Uganda Are Highly Diverse but Dominated by Enterobacteriaceae

Roßmann

Müller

Smalla

et al. 2012

Appl Environ Microbiol

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ABSTRACTBananas are among the most widely consumed foods in the world. In Uganda, the country with the second largest banana production in the world, bananas are the most important staple food. The objective of this study was to analyze banana-associated microorganisms and to select efficient antagonists against fungal pathogens which are responsible for substantial yield losses. We studied the structure and function of microbial communities (endosphere, rhizosphere, and soil) obtained from three different traditional farms in Uganda by cultivation-independent (PCR-SSCP fingerprints of 16S rRNA/ITS genes, pyrosequencing of enterobacterial 16S rRNA gene fragments, quantitative PCR, fluorescencein situhybridization coupled with confocal laser scanning microscopy, and PCR-based detection of broad-host-range plasmids and sulfonamide resistance genes) and cultivation-dependent methods. The results showed microhabitat-specific microbial communities that were significant across sites and treatments. Furthermore, all microhabitats contained a high number and broad spectrum of indigenous antagonists toward identified fungal pathogens. While bacterial antagonists were found to be enriched in banana plants, fungal antagonists were less abundant and mainly found in soil. The banana stem endosphere was the habitat with the highest bacterial counts (up to 109gene copy numbers g−1). Here, enterics were found to be enhanced in abundance and diversity; they provided one-third of the bacteria and were identified by pyrosequencing with 14 genera, including not only potential human (Escherichia,Klebsiella,Salmonella, andYersiniaspp.) and plant (Pectobacteriumspp.) pathogens but also disease-suppressive bacteria (Serratiaspp.). The dominant role of enterics can be explained by the permanent nature and vegetative propagation of banana and the amendments of human, as well as animal, manure in these traditional cultivations.

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mentioning

confidence: 99%

Banana-Associated Microbial Communities in Uganda Are Highly Diverse but Dominated by Enterobacteriaceae

Roßmann

Müller

Smalla

et al. 2012

Appl Environ Microbiol

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“…As a result, very little is known about the overall diversity and composition of microbial communities on harvested produce and how these communities vary across produce types. Based on recent studies on this topic (Rudi et al, 2002;Ponce et al, 2008;Ottesen et al, 2009;Rastogi et al, 2012;Leff and Fierer, 2013;), a few key patterns are emerging: (1) different produce types and cultivars can harbor different levels (abundances) of specific microbial groups (Critzer and Doyle, 2010), (2) farming and storage conditions can influence the composition and abundances of microbial communities found on produce, and (3) non-pathogenic microbes can interact with and inhibit microbial pathogens found on produce surfaces (Shi et al, 2009;Critzer and Doyle, 2010;Teplitski et al, 2011). Despite this recent body of work, we still have a limited understanding of the diversity of produce-associated microbial communities, their function, the factors that influence the composition of these communities after harvest and during storage, and the distribution of individual taxa (particularly those taxa that are difficult to culture) across different commodities.…”

Section: The Role Of the Microbiome In Fruit Health And Disease à A Nmentioning

confidence: 99%

The science, development, and commercialization of postharvest biocontrol products

Droby

Wisniewski

Teixidó

et al. 2016

Postharvest Biology and Technology

287

149

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A B S T R A C TPostharvest biological control agents as a viable alternative to the use of synthetic chemicals have been the focus of considerable research for the last 30 years by many scientists and several commercial companies worldwide. Several antagonists of postharvest pathogens have been identified and tested in laboratory, semi-commercial, and commercial settings and were developed into commercial products. The discovery and development of these antagonists into a product followed a paradigm in which a single antagonist isolated from one commodity was also expected to be effective on other commodities that vary in their genetic background, physiology, postharvest handling, and susceptibility to pathogens. In most cases, product development was successfully achieved but their full commercial potential was not realized. The low success rate of postharvest biocontrol products has been attributed to several problems, including difficulties in mass production and formulation of the antagonist, the physiological status of the harvested commodity and its susceptibility to specific pathogens. All these factors played a major role in the reduced and inconsistent performance of the biocontrol product when used under commercial conditions. Although many studies have been conducted on the mode of action of postharvest microbial antagonists, our understanding is still very incomplete. In this regard, a systems approach, that takes into account all the components of the biocontrol system, may represent the best approach to investigating the network of interactions that exist. Very little is known about the overall diversity and composition of microbial communities on harvested produce and how these communities vary across produce types, their function, the factors that influence the composition of the microbiota after harvest and during storage, and the distribution of individual taxa. In light of the progress made in recent years in metagenomic technologies, this technology should be used to characterize the composition of microbial communities on fruit and vegetables. Information on the dynamics and diversity of microbiota may be useful to developing a new paradigm in postharvest biocontrol that is based on constructing synthetic microbial communities that provide superior control of pathogens.

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“…Pathogenic bacteria (e.g., Listeria monocytogenes, Escherichia coli, Salmonella) can also be part of larger microbial communities on fresh produce (Shi et al, 2009;Teplitski et al, 2011). Field surveys on contamination of produce with pathogenic bacteria such as E. coli O157:H7, Salmonella spp., and L. monocytogenes found drastically different results depending on the methods, location, seasons, produce type, and criteria of the survey conducted.…”

Section: Prevalence and Diversity Of Microbial Communities On Fresh Vmentioning

confidence: 99%

Microbial ecology of fresh vegetables

Zheng

Kase

Jesús

et al. 2016

Quantitative Microbiology in Food Processing

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Untangling metabolic and communication networks: interactions of enterics with phytobacteria and their implications in produce safety

Cited by 77 publications

References 63 publications

Banana-Associated Microbial Communities in Uganda Are Highly Diverse but Dominated by Enterobacteriaceae

Banana-Associated Microbial Communities in Uganda Are Highly Diverse but Dominated by Enterobacteriaceae

The science, development, and commercialization of postharvest biocontrol products

Microbial ecology of fresh vegetables

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