Untangling factors that drive community composition of root associated fungal endophytes of Neotropical epiphytic orchids

Cevallos, Stefania; Herrera, Paulo; Sánchez-Rodríguez, Aminael; Declerck, Stéphane; Suárez, Juan Pablo

doi:10.1016/j.funeco.2018.05.002

Cited by 23 publications

(23 citation statements)

References 52 publications

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“…For all three Bletilla species, the putative life strategies with the top three were saprotrophs, pathotroph-saprotroph, and pathotroph-saprotrophsymbiotroph. The proportions of saprotrophic fungi for three Bletilla species ranged from 44% to 56%, which were similar to the results of Cevallos et al [50]. Cevallos et al found that saprotrophs occupied the largest proportion of 45% in the roots of neotropical epiphytic orchids in the southern Ecuadorian Andes.…”

Section: The Functional Roles Of Root-associated Fungal Endophytessupporting

confidence: 85%

Root Endophytic Fungal Community and Carbon and Nitrogen Stable Isotope Patterns Differ among Bletilla Species (Orchidaceae)

Zeng

Diao

et al. 2021

JoF

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Orchids of the genus Bletilla are well-known ornamental plants and sources of traditional medicine in Asia that rely on the symbiotic relationship with root endophytic fungi throughout their whole life cycle. However, little is known about their fungal partners, infection pattern, and pathways of carbon gain. We investigated carbon and nitrogen stable isotope patterns in different organs of three Bletilla species, identified the root endophytic fungal community composition, and determined mycorrhizal colonization rates. The three Bletilla species were comprised by a polyphyletic group which belongs to different trophic modes, such as saprotroph, pathotroph, and symbiotroph; however, the dominant species and their abundances varied among Bletilla spp. Mycorrhizal infection rates also varied among Bletilla species, with B. striata (65% ± 25%) being significantly higher than those of B. formosana (35% ± 16%) and B. ochracea (22% ± 13%). Compared with surrounding autotrophic plants, all Bletilla spp. were significantly enriched in 13C with B. striata to a significantly higher level than other two Bletilla species. Among different organs, stems had higher δ13C values, while leaves and flowers had higher δ15N and total N content values across all three species. Our results indicate that the symbiotic relationship of Bletilla and its root endophytic fungi is not strictly specific. Although mycorrhizal infection rates were highly variable, the three Bletilla species had the same infection pattern with hyphae penetrating the cortex cell by the pathway cell. Different Bletilla species have different strategies for C allocation among plant organs. These findings provide new insights into the ecological adaptation of orchids and will contribute to Bletilla germplasm conservation and sustainable utilization.

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Section: The Functional Roles Of Root-associated Fungal Endophytessupporting

confidence: 85%

Root Endophytic Fungal Community and Carbon and Nitrogen Stable Isotope Patterns Differ among Bletilla Species (Orchidaceae)

Zeng

Diao

et al. 2021

JoF

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“…In the past half-decade ITS3/ITS4OF were frequently used for identifying mycorrhizal fungal communities in orchid roots of terrestrial orchids and the surrounding soils (Jacquemyn et al, 2015a(Jacquemyn et al, , 2017bEsposito et al, 2016;Duffy et al, 2019). Additionally, ITS86F/ITS4 is still used for the detection of mycorrhizal partners of epiphytic orchids (Cevallos et al, 2017(Cevallos et al, , 2018aHerrera et al, 2019b;Izuddin et al, 2019;Jacquemyn et al, 2021).…”

Section: Primer Selection In Htsmentioning

confidence: 99%

“…Orchids are often associated with phylogenetically and ecologically diverse fungi. Basidiomycota and Ascomycota, with very few Chytridiomycota, Glomeromycota, Zygomycota, or Mucoromycota, are widely distributed in the aerial roots of epiphytic orchids and in the underground roots or rhizomes of terrestrial and lithophytic orchids (Martos et al, 2012;Waud et al, 2014;Cevallos et al, 2018a;Egidi et al, 2018;Novotná et al, 2018;Qin et al, 2019). Fungi in tissues can be divided into true OMF and orchid non-mycorrhizal fungi (ONF) based on the structures formed during in orchids.…”

Section: Orchid-fungal Diversitymentioning

confidence: 99%

“…Studies on high-altitude tropical southern Ecuador montane rainforests reported that some epiphytic orchids have higher richness or abundance of OMF at relatively high altitudes (Cevallos et al, 2018a;Herrera et al, 2019b). However, Cevallos et al (2018a) insisted that high-altitude areas (3000-3,500 m) showed no significant difference in fungal communities among different orchid species or sites, while in low-altitude areas (2,050-2,800 m), host orchids and sites were the two major drivers of OMF communities' composition. Cevallos et al (2018b) suggest that OMF display rich diversity in mid-altitude areas (approximately 2,050 m), but this is limited to a narrow altitude range (1,850-2,100 m).…”

Section: Difference and Adaptability Of Omf In Different Habitatsmentioning

confidence: 99%

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Progress and Prospects of Mycorrhizal Fungal Diversity in Orchids

Yang

et al. 2021

Front. Plant Sci.

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Orchids form mycorrhizal symbioses with fungi in natural habitats that affect their seed germination, protocorm growth, and adult nutrition. An increasing number of studies indicates how orchids gain mineral nutrients and sometime even organic compounds from interactions with orchid mycorrhizal fungi (OMF). Thus, OMF exhibit a high diversity and play a key role in the life cycle of orchids. In recent years, the high-throughput molecular identification of fungi has broadly extended our understanding of OMF diversity, revealing it to be a dynamic outcome co-regulated by environmental filtering, dispersal restrictions, spatiotemporal scales, biogeographic history, as well as the distribution, selection, and phylogenetic spectrum width of host orchids. Most of the results show congruent emerging patterns. Although it is still difficult to extend them to all orchid species or geographical areas, to a certain extent they follow the “everything is everywhere, but the environment selects” rule. This review provides an extensive understanding of the diversity and ecological dynamics of orchid-fungal association. Moreover, it promotes the conservation of resources and the regeneration of rare or endangered orchids. We provide a comprehensive overview, systematically describing six fields of research on orchid-fungal diversity: the research methods of orchid-fungal interactions, the primer selection in high-throughput sequencing, the fungal diversity and specificity in orchids, the difference and adaptability of OMF in different habitats, the comparison of OMF in orchid roots and soil, and the spatiotemporal variation patterns of OMF. Further, we highlight certain shortcomings of current research methodologies and propose perspectives for future studies. This review emphasizes the need for more information on the four main ecological processes: dispersal, selection, ecological drift, and diversification, as well as their interactions, in the study of orchid-fungal interactions and OMF community structure.

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“…OFEs located inside roots of Orchidaceae in the cortical or in the velamen tissues are cooperatively called as Orchidaceae root-associated fungal endophytes (ORAFEs). However, research focusing on ORAFEs normally ignore the strains occurred in the velamen due to which the profuse corky epidermis is usually known as an adaptive assembly for conservation [33]. Therefore, the roots cortical tissues are the chief target of numerous researches focusing on ORAFEs because it frequently anchorages fungal endophytes that may form network with the Orchidaceae as symbionts [34].…”

Section: Orchidaceae Rootsmentioning

confidence: 99%

A comprehensive review on fungal endophytes and its dynamics on Orchidaceae plants: current research, challenges, and future possibilities

2019

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In the development of medicinally important Orchidaceae, the extent of fungal endophytes specificity is not presently very clear. Limited study has been available on natural products formed and its role on plant growth, defence mechanism by endophytes, and to characterize the chief treasure of bioactive molecules. Therefore, this review article presents an evaluation of the endophytes associated with Orchidaceae for physiology, metabolism, and genomics which have prominently contributed to the resurgence of novel metabolite research increasing our considerate of multifaceted mechanisms regulatory appearance of biosynthetic gene groups encoding diverse metabolites. Additionally, we presented the comprehensive recent development of biostrategies for the cultivation of endophytes from Orchidaceae and integration of bioengineered 'Genomics with metabolism' approaches with emphases collective omics as powerful approach to discover novel metabolite compounds. The Orchidaceae-fungal endophytes' biodynamics for sustainable development of bioproducts and its applications are supported in large-scale biosynthesis of industrially and pharmaceutical important biomolecules. ARTICLE HISTORY

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Untangling factors that drive community composition of root associated fungal endophytes of Neotropical epiphytic orchids

Cited by 23 publications

References 52 publications

Root Endophytic Fungal Community and Carbon and Nitrogen Stable Isotope Patterns Differ among Bletilla Species (Orchidaceae)

Root Endophytic Fungal Community and Carbon and Nitrogen Stable Isotope Patterns Differ among Bletilla Species (Orchidaceae)

Progress and Prospects of Mycorrhizal Fungal Diversity in Orchids

A comprehensive review on fungal endophytes and its dynamics on Orchidaceae plants: current research, challenges, and future possibilities

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