1972
DOI: 10.1016/0014-4827(72)90488-0
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Unscheduled DNA synthesis in hen erythrocyte nuclei reactivated in heterokaryons

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Cited by 26 publications
(4 citation statements)
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“…The human factor that restores UDS also enters chick erythrocyte nuclei during reactivation in HeLa cytoplasm, but not in XP fibroblast cells (194). The chick nucleus itself does not complement the deficiency in XP nuclei even after reactivation.…”
Section: Dna Repair In Fused Cellsmentioning
confidence: 94%
“…The human factor that restores UDS also enters chick erythrocyte nuclei during reactivation in HeLa cytoplasm, but not in XP fibroblast cells (194). The chick nucleus itself does not complement the deficiency in XP nuclei even after reactivation.…”
Section: Dna Repair In Fused Cellsmentioning
confidence: 94%
“…Chick erythrocytes were obtained from 15-to 16-day-old embryos as described (7,11). HeLa cell cultures were maintained in Eagle's medium containing 10% fetal bovine serum and antibiotics (penicillin 100 u/ml; streptomycin 100 ,ug/ ml).…”
Section: Methodsmentioning
confidence: 99%
“…HeLa cell cultures were maintained in Eagle's medium containing 10% fetal bovine serum and antibiotics (penicillin 100 u/ml; streptomycin 100 ,ug/ ml). Heterokaryons were prepared by fusion of chick erythrocytes with HeLa cells using UV-inactivated Sendai virus (800-1600 hemagglutinating units/ml) according to a technique described (7,11 tion). Only heterokaryons containing one chick-erythrocyte nucleus and one HeLa nucleus were counted.…”
Section: Methodsmentioning
confidence: 99%
“…ting to note that Darzynkiewicz and Chelmicka-Szorc [1972] could detect no unscheduled DNA synthesis following UV irradiation of dormant hen erythrocytes -a highly differentiated cell. When dormant erythrocyte nuclei were reactivated by fusion with HeLa cells or rat fibroblasts, however, their repair capacity was restored within 16 h. This effect appeared to result from the migration of repair enzymes into the reactivated nuclei, rather than to an activation of the erythrocyte's own repair processes.…”
Section: Differentiating Cellsmentioning
confidence: 99%