Unsaturated fatty acids and their oxidation products stimulate CD36 gene expression in human macrophages

Vallvé, Joan-Carles; Uliaque, K.; Girona, Josefa; Cabré, Anna; Ribalta, Josep; Heras, Mercedes; Masana, Luís

doi:10.1016/s0021-9150(02)00046-1

Cited by 63 publications

(41 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pretreatment of the myotubes with OA did not significantly increase fatty acid uptake, but the expression of CD36/FAT increased similarly to that in EPA-exposed cells. It has been observed that PUFAs and their oxidation products stimulate the expression of CD36/FAT in human macrophages (29), most likely through the activation of PPARg, and the activation of p38 mitogen-activated protein kinase has been shown to be involved in the regulation of CD36/FAT through PPARg (30). On the other hand, activation of CD36/FAT has been shown to induce the phosphorylation of both ERK and p38 (31)(32)(33).…”

Section: Discussionmentioning

confidence: 99%

Eicosapentaenoic acid (20:5 n-3) increases fatty acid and glucose uptake in cultured human skeletal muscle cells

Aas

Rokling-Andersen

Kase

et al. 2006

Journal of Lipid Research

View full text Add to dashboard Cite

This study was conducted to evaluate the chronic effects of eicosapentaenoic acid (EPA) on fatty acid and glucose metabolism in human skeletal muscle cells. Uptake of [ 14 C]oleate was increased .2-fold after preincubation of myotubes with 0.6 mM EPA for 24 h, and incorporation into various lipid classes showed that cellular triacylgycerol (TAG) and phospholipids were increased 2-to 3-fold compared with control cells. After exposure to oleic acid (OA), TAG was increased 2-fold. Insulin (100 nM) further increased the incorporation of [ 14 C]oleate into all lipid classes for EPA-treated myotubes. Fatty acid b-oxidation was unchanged, and complete oxidation (CO 2 ) decreased in EPA-treated cells. Basal glucose transport and oxidation (CO 2 ) were increased 2-fold after EPA, and insulin (100 nM) stimulated glucose transport and oxidation similarly in control and EPA-treated myotubes, whereas these responses to insulin were abolished after OA treatment. Lower concentrations of EPA (0.1 mM) also increased fatty acid and glucose uptake. CD36/FAT (fatty acid transporter) mRNA expression was increased after EPA and OA treatment compared with control cells. Moreover, GLUT1 expression was increased 2.5-fold by EPA, whereas GLUT4 expression was unchanged, and activities of the mitogen-activated protein kinase p38 and extracellular signal-regulated kinase were decreased after treatment with OA compared with EPA. Together, our data show that chronic exposure of myotubes to EPA promotes increased uptake and oxidation of glucose despite a markedly increased fatty acid uptake and synthesis of complex lipids.-Aas, V

show abstract

Section: Discussionmentioning

confidence: 99%

Eicosapentaenoic acid (20:5 n-3) increases fatty acid and glucose uptake in cultured human skeletal muscle cells

Aas

Rokling-Andersen

Kase

et al. 2006

Journal of Lipid Research

View full text Add to dashboard Cite

show abstract

“…In the hepatopancreas of Atlantic salmon, the expression of FAT/CD36 was not affected by vegetable or FOs , while in mammals, unsaturated LCFAs significantly increased FAT/CD36 mRNA levels in macrophages and ventricular myocytes (van der Lee et al 2000;Vallvé et al 2002), the small intestine, preadipocytes and neonatal cardiomiocytes (Chen et al 2001), mature adipose cells (Nisoli et al 2000) and abdominal fat of hypertensive rats (Aguilera et al 2006). Furthermore, Yang et al (2007) showed that FAs with a chain length longer than eight carbons could effectively inhibit FAT/CD36 expression in adipocytes, but unsaturated LCFAS had no effect.…”

Section: Fat/cd36 Mrna Expression Responses To Different Dietary Oil mentioning

confidence: 99%

LCFA Uptake and FAT/CD36: molecular cloning, tissue expression and mRNA expression responses to dietary oil sources in grass carp (Ctenopharyngodon idellus)

Zhou

Lin

et al. 2017

Journal of Applied Animal Research

View full text Add to dashboard Cite

“…In contrast, Vlallve et al observed that acid EPA, DHA and to a lesser extent oleic and linoleic acids significantly enhanced CD36 expression in THP-1 and monocyte-derived-macrophages both at the transcriptomic and protein levels. However, a significant reduction in CD36 expression was noted with oxidation products (aldehydes) of fatty-acids such as 4-hydroxynonenal (HNE) and hexanal, 2,4-decadienal (DDE) [83].…”

Section: Cd36 As a Potential Therapeutic Targetmentioning

confidence: 99%

CD36 and macrophages in atherosclerosis

Collot-Teixeira

Martin

McDermott-Roe

et al. 2007

Cardiovascular Research

332

256

View full text Add to dashboard Cite

CD36 is a multi-ligand scavenger receptor present on the surface of a number of cells such as platelets, monocytes/macrophages, endothelial and smooth muscle cells. Monocyte/macrophage CD36 has been shown to play a critical role in the development of atherosclerotic lesions by its capacity to bind and endocytose oxidized low density lipoproteins (OxLDL), and it is implicated in the formation of foam cells. However, the significance of CD36 in atherosclerosis has recently been called into question by different studies, and therefore its exact role still needs to be clarified. The aim of this article is to carefully review the importance of CD36 as an essential component in the pathogenesis of atherosclerosis. © 2007 European Society of Cardiology. Published by Elsevier B.V. All rights reserved.Keywords: CD36; Vascular; Monocyte/macrophages; OxLDL; Atherosclerosis Atherosclerosis is a progressive chronic inflammatory disease characterised by a gradual thickening and hardening of arteries that ultimately leads to a reduction in the lumen diameter and potentially to ischemia following plaque rupture. A first stage of the disease is the presence of dysfunctional endothelial cells (EC) which via activated adhesion molecules and expressed chemokines recruit circulating monocytes and a subpopulation of lymphocytes (CD4/CD8) into the intima. Endothelial dysfunction may be induced by oxidized low density lipoproteins (OxLDL). Indeed, LDL when infiltrating into the intima can be readily oxidized by resident macrophages or endothelial cells. Moreover, C-reactive protein (CRP) and OxLDL can act synergistically to increase monocytes inflammatory properties (through MCP-1, PGE2, MMP-1 production) and attract further circulating monocytes through the release of MCP-1 to adhere to the activated dysfunctional endothelial cells and extravasate to the intima to scavenge OxLDL [1]. One of the involvements of CD36 in lesion development is its ability to bind and endocytose OxLDL into macrophages, as a scavenger receptor, and ultimately be implicated in the differentiation of resident macrophages into foam cells that constitute the atherosclerotic lesion core.CD36 is an 88-kD membrane glycoprotein that was first identified on monocytes by monoclonal antibody OKM5 [2] and then subsequently isolated from blood platelets [3,4]. This membrane glycoprotein is expressed by many celltypes including microvasculature endothelial [5] and smooth muscle cells (SMC) [6]. Functional and structural characterisation showed CD36 to be a member of the scavenger receptor class B family with a capacity to bind OxLDL as well as various other ligands. The importance of monocytic CD36 in the initiation and perpetuation of atherosclerotic lesions was shown over this past decade by its effect in significantly reducing the size of vascular lesions when

show abstract

Unsaturated fatty acids and their oxidation products stimulate CD36 gene expression in human macrophages

Cited by 63 publications

References 51 publications

Eicosapentaenoic acid (20:5 n-3) increases fatty acid and glucose uptake in cultured human skeletal muscle cells

Eicosapentaenoic acid (20:5 n-3) increases fatty acid and glucose uptake in cultured human skeletal muscle cells

LCFA Uptake and FAT/CD36: molecular cloning, tissue expression and mRNA expression responses to dietary oil sources in grass carp (Ctenopharyngodon idellus)

CD36 and macrophages in atherosclerosis

Contact Info

Product

Resources

About