Unravelling the Role of LncRNA WT1-AS/miR-206/NAMPT Axis as Prognostic Biomarkers in Lung Adenocarcinoma

Abstract

Lung cancer is the world’s highest morbidity and mortality of malignant tumors, with lung adenocarcinoma (LUAD) as a major subtype. The competitive endogenous RNA (ceRNA) regulative network provides opportunities to understand the relationships among different molecules, as well as the regulative mechanisms among them in order to investigate the whole transcriptome landscape in cancer pathology. We designed this work to explore the role of a key oncogene, MYC, in the pathogenesis of LUAD, and this study aims to identify important long noncoding RNA (lncRNA)-microRNA (miRNA)- transcription factor (TF) interactions in non-small cell lung cancer (NSCLC) using a bioinformatics analysis. The Cancer Genome Atlas (TCGA) database, containing mRNA expression data of NSCLC, was used to determine the deferentially expressed genes (DEGs), and the ceRNA network was composed of WT1-AS, miR-206, and nicotinamide phosphoribosyltransferase (NAMPT) bashing on the MYC expression level. The Kaplan–Meier univariate survival analysis showed that these components may be closely related prognostic biomarkers and will become new ideas for NSCLC treatment. Moreover, the high expression of WT1-AS and NAMPT and low expression of miR-206 were associated with a shortened survival in NSCLC patients, which provided a survival advantage. In summary, the current study constructing a ceRNA-based WT1-AS/miR-206/NAMPT axis might be a novel important prognostic factor associated with the diagnosis and prognosis of LUAD.

Comprehensive Analysis of CDK1-Associated ceRNA Network Revealing the Key Pathways LINC00460/LINC00525-Hsa-Mir-338-FAM111/ZWINT as Prognostic Biomarkers in Lung Adenocarcinoma Combined with Experiments

“…The functions of long non-coding RNAs (lncRNAs) have been proved in protecting chromosome integrity, maintaining genome structure, transcription, translation and epigenetic regulation [ 8 , 9 , 10 ]. Imbalances of lncRNA are also associated with the development of many diseases and complications, such as premature preterm rupture of membrane, autism spectrum disorders, and cancer [ 11 , 12 , 13 , 14 ]. MicroRNAs (miRNAs) target and induce translation inhibition or degradation of mRNA to control gene expression and regulate biological processes [ 15 , 16 ].…”

BAL Proteomic Signature of Lung Adenocarcinoma in IPF Patients and Its Transposition in Serum Samples for Less Invasive Diagnostic Procedures

“…Our results do not report NAMPT in BALF samples; rather, it is detected only in serum, evidencing a lower abundance in LC-IPF with respect to IPF and CTRLs. NAMPT is frequently studied in lung cancer, particularly in NSCLC, and it is considered a potential target of treatment [ 73 , 74 ], as well as a prognostic biomarker [ 75 ]. Its characteristic low abundance in serum samples of LC-IPF patients could be tested in a wider cohort of samples in order to render our data more robust.…”

WT1-AS/IGF2BP2 Axis Is a Potential Diagnostic and Prognostic Biomarker for Lung Adenocarcinoma According to ceRNA Network Comprehensive Analysis Combined with Experiments

“…The lncRNA (WT1-AS)–miRNA (mir-200a-3p)–mRNA (IGF2BP2) regulatory axis associated with LUAD prognosis was identified based on the survival-related analysis and differential expression results of RNAs. Although previous studies have extensively reported the role of WT1-AS in different types of cancer, the role of WT1-AS in tumors remains incompletely defined [ 37 , 38 ]. For example, downregulation of WT1-AS can promote the proliferation and invasiveness of gastric cancer cells [ 39 ].…”