Unravelling the Prunus/Plum pox virus interactions

Abstract

Plum pox virus (PPV) belongs to the genus Potyvirus that contains the largest number of virus species infecting plants. Its virus genome has been extensively characterised and sequenced. However, few data are available on its interactions with woody host plants. We therefore focused, in the past 4 years, both on the cellular and molecular aspects of the compatible and incompatible Prunus /PPV interactions. GFP (Green fluorescent protein)-tagged PPV and in situ hybridisation were used to compare the localization of viral particles in stems and leaves of susceptible and resistant apricot cultivars. In parallel, molecular tools were developed through the cloning and characterization of polymorphic, homologous resistance genes and of candidate genes involved in the expression of Prunus /PPV interactions. Candidate genes are currently used to target genomic regions involved in resistance or susceptibility and to identify molecular markers indispensable for further marker assisted selection for resistance to sharka disease.

Quantitative trait loci meta‐analysis of Plum pox virus resistance in apricot (Prunus armeniaca L.): new insights on the organization and the identification of genomic resistance factors

“…2). Indeed, when ‘Stark Early Orange’ was challenged with eight distinct PPV isolates, no difference in resistance level was observed (Decroocq et al ., 2006a). We can therefore hypothesize that these QTLs and, consequently, the metaQTLs display a broad‐spectrum range of resistance to PPV, suggesting that a conserved viral determinant may be recognized by the plant.…”

Reaction of an F1 apricot hybrid population to artificial and natural inoculation of Plum pox virus

“…No systemic PPV infection was detected in the scion of 'Stark Early Orange' inoculated with eight different PPV isolates, corresponding to the three major serotypes 'Marcus', 'Dideron' and 'El Amar' in a six-year study (Decroocq et al 2006).…”

Detection, Understanding and Controlof Soybean Mosaic Virus

“…Silencing or mutation of host factors required for virus infection will generate genetic resistance to the virus. Over the last decade, a number of host factors required for potyvirus infection have been identified and examples include eIF4E, eIF(iso)4E, eIF4G, PABP, AtRH8 and PpDDX (Lellis et al, 2002;Ruffel et al, 2002Ruffel et al, , 2005Nicaise et al, 2003Nicaise et al, , 2007Gao et al, 2004;Kang et al, 2005;Decroocq et al, 2006;Bruun-Rasmussen et al, 2007;Dufresne et al, 2008;Huang et al, 2010). Recently Piron et al (2010) have reported a successful story about how to deploy host factors for the development of resistance to potyviruses in tomato.…”