Unravelling the core microbiome of biofilms in cooling tower systems

Gregorio, L Di; Tandoi, Valter; Congestri, Roberta; Rossetti, Simona; Pippo, Francesca Di

doi:10.1080/08927014.2017.1367386

Cited by 38 publications

(22 citation statements)

References 32 publications

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“…Therefore, interfering with biofilm formation or stimulating its dissociation is an attractive strategy to combat bacterial infections and preventing their chronic development. However, biofilms are not just a major problem in clinics, but also in agriculture because of plant loss due to bacterial diseases ( Ramey et al, 2004 ; Matthysse et al, 2005 ; Koczan et al, 2009 ; Malamud et al, 2012 ; Velmourougane et al, 2017 ), and in industrial water, gas and oil systems, where microbial-induced corrosion causes pipe leakage ( Wang et al, 2013 ; Ramírez et al, 2016 ; Di Gregorio et al, 2017 ).…”

Section: Role Of D -Amino Acids In Microbial Commumentioning

confidence: 99%

New Insights Into the Mechanisms and Biological Roles of D-Amino Acids in Complex Eco-Systems

2018

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In the environment bacteria share their habitat with a great diversity of organisms, from microbes to humans, animals and plants. In these complex communities, the production of extracellular effectors is a common strategy to control the biodiversity by interfering with the growth and/or viability of nearby microbes. One of such effectors relies on the production and release of extracellular D-amino acids which regulate diverse cellular processes such as cell wall biogenesis, biofilm integrity, and spore germination. Non-canonical D-amino acids are mainly produced by broad spectrum racemases (Bsr). Bsr’s promiscuity allows it to generate high concentrations of D-amino acids in environments with variable compositions of L-amino acids. However, it was not clear until recent whether these molecules exhibit divergent functions. Here we review the distinctive biological roles of D-amino acids, their mechanisms of action and their modulatory properties of the biodiversity of complex eco-systems.

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Section: Role Of D -Amino Acids In Microbial Commumentioning

confidence: 99%

New Insights Into the Mechanisms and Biological Roles of D-Amino Acids in Complex Eco-Systems

2018

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“…Biofilms from full-scale CTs are generally collected from the wall of the CT basins, which is the most accessible location of the system and does not require interruption of operation (Di Gregorio et al 2017). The present pilot study is the first describing analyses of microbial communities from different locations in the recirculation line of a nondisinfected CT.…”

Section: Comparison Of Biofilm Microbial Composition Along the Coolinmentioning

confidence: 99%

“…No literature is, however, available on the effectiveness of P-limitation in controlling biofouling in cooling water systems. A few studies have investigated the microbiome of biofilms from CTs (Balamurugan et al 2011;Wang et al 2013;Di Gregorio et al 2017;Tsao et al 2019), mainly focusing on MIC and the impact of disinfection, but the effect of nutrient limitations and sample location remains unexplored. In general, further investigation on the effect of nutrient limitations in cooling water would be highly valuable to the industrial field as it guides the selection of feed water, pre-treatment and chemical dosage strategies.…”

Section: Introductionmentioning

confidence: 99%

Effect of phosphate availability on biofilm formation in cooling towers

et al. 2020

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Phosphate limitation has been suggested as a preventive method against biofilms. P-limited feed water was studied as a preventive strategy against biofouling in cooling towers (CTs). Three pilot-scale open recirculating CTs were operated in parallel for five weeks. RO permeate was fed to the CTs (1) without supplementation (reference), (2) with supplementation by biodegradable carbon (P-limited) and (3) with supplementation of all nutrients (non-P-limited). The P-limited water contained 10 mg PO 4 l À1. Investigating the CT-basins and coupons showed that P-limited water (1) did not prevent biofilm formation and (2) resulted in a higher volume of organic matter per unit of active biomass compared with the other CTs. Exposure to external conditions and cycle of concentration were likely factors that allowed a P concentration sufficient to cause extensive biofouling despite being the limiting compound. In conclusion, phosphate limitation in cooling water is not a suitable strategy for CT biofouling control.

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“…Biofilm 103 plays a crucial role in Legionella proliferation and survival (Cooper and Hanlon, 2010;104 Flemming et al, 2002;Rogers and Keevil, 1992;Simões et al, 2010). In addition, the 105 composition of the microbial communities in water systems is different in the biofilm and in the 106 water phase (Di Gregorio et al, 2017;Wang et al, 2014). Therefore, analysing the microbial 107 interaction between Lp and the resident microbiota in the water and in the biofilm is warranted to 108 fully understand its life cycle and propose better strategies to control its growth.…”

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confidence: 99%

Impact of temperature onLegionella pneumophila, its protozoan host cells, and the microbial diversity of the biofilm community of a pilot cooling tower

Paniagua

Paranjape

et al. 2019

Preprint

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Legionella pneumophila (Lp) is a waterborne bacterium known for causing Legionnaires’ Disease, a severe pneumonia. Cooling towers are a major source of outbreaks, since they provide ideal conditions for Lp growth and produce aerosols. In such systems, Lp typically grow inside protozoan hosts. Several abiotic factors such as water temperature, pipe material and disinfection regime affect the colonization of cooling towers by Lp. The local physical and biological factors promoting the growth of Lp in water systems and its spatial distribution are not well understood. Therefore, we built a lab-scale cooling tower to study the dynamics of Lp colonization in relationship to the resident microbiota and spatial distribution. The pilot was filled with water from an operating cooling tower harboring low levels of Lp. It was seeded with Vermamoeba vermiformis, a natural host of Lp, and then inoculated with Lp. After 92 days of operation, the pilot was disassembled, the water was collected, and biofilm was extracted from the pipes. The microbiome was studied using 16S rRNA and 18S rRNA genes amplicon sequencing. The communities of the water and of the biofilm were highly dissimilar. The relative abundance of Legionella in water samples reached up to 11% whereas abundance in the biofilm was extremely low (≤0.5 %). In contrast, the host cells were mainly present in the biofilm. This suggest that Lp grows in host cells associated with biofilm and is then released back into the water following host cell lysis. In addition, water temperature shaped the bacterial and eukaryotic community of the biofilm, indicating that different parts of the systems may have different effects on Legionella growth.

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Unravelling the core microbiome of biofilms in cooling tower systems

Cited by 38 publications

References 32 publications

New Insights Into the Mechanisms and Biological Roles of D-Amino Acids in Complex Eco-Systems

New Insights Into the Mechanisms and Biological Roles of D-Amino Acids in Complex Eco-Systems

Effect of phosphate availability on biofilm formation in cooling towers

Impact of temperature onLegionella pneumophila, its protozoan host cells, and the microbial diversity of the biofilm community of a pilot cooling tower

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