Unraveling Trichoderma species in the attine ant environment: description of three new taxa

Montoya, Quimi Vidaurre; Meirelles, Lucas A.; Chaverri, Priscila; Rodrigues, André

doi:10.1007/s10482-016-0666-9

Cited by 45 publications

(36 citation statements)

References 64 publications

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“…Thus, for species-level identification, the tef-1 region has been widely used in the taxonomy and systematics of this genus. 123,150−153 The tef-1 intron 4, in combination with intron 5, is proving to be very useful for species-level identification. 154,155 In addition, a 1200 bp fragment of RPB2 , which can be amplified and sequenced using primer pairs RPB2–5f and RPB2–7cR, 156 is also proving useful for Trichoderma identification using phylogenetic methods.…”

Section: The Various Protein-coding Genes Used To Augment or Supplantmentioning

confidence: 99%

Fungal Identification Using Molecular Tools: A Primer for the Natural Products Research Community

et al. 2017

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Fungi are morphologically, ecologically, metabolically, and phylogenetically diverse. They are known to produce numerous bioactive molecules, which makes them very useful for natural products researchers in their pursuit of discovering new chemical diversity with agricultural, industrial, and pharmaceutical applications. Despite their importance in natural products chemistry, identification of fungi remains a daunting task for chemists, especially those who do not work with a trained mycologist. The purpose of this review is to update natural products researchers about the tools available for molecular identification of fungi. In particular, we discuss (1) problems of using morphology alone in the identification of fungi to the species level; (2) the three nuclear ribosomal genes most commonly used in fungal identification and the potential advantages and limitations of the ITS region, which is the official DNA barcoding marker for species-level identification of fungi; (3) how to use NCBI-BLAST search for DNA barcoding, with a cautionary note regarding its limitations; (4) the numerous curated molecular databases containing fungal sequences; (5) the various protein-coding genes used to augment or supplant ITS in species-level identification of certain fungal groups; and (6) methods used in the construction of phylogenetic trees from DNA sequences to facilitate fungal species identification. We recommend that, whenever possible, both morphology and molecular data be used for fungal identification. Our goal is that this review will provide a set of standardized procedures for the molecular identification of fungi that can be utilized by the natural products research community.

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Section: The Various Protein-coding Genes Used To Augment or Supplantmentioning

confidence: 99%

Fungal Identification Using Molecular Tools: A Primer for the Natural Products Research Community

et al. 2017

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“…14.13). More recently, In a large-scale survey of Trichoderma from rotten wood and soil in China, Zhuang (2016a, b, c, d, e, 2017) published 27 new species based on the collections producing ascomata or asexual morphs on woody substrates; Chen and Zhuang (2016) described two new species based on soil samples from the Hubei and Tibet regions of China; Montoya et al (2016) found three new taxa in the attine ant environment; Sun et al (2016) described a new fungicolous Trichoderma species which was isolated from surface of the stroma of Hypoxylon anthochroum. Until now, 287 Trichoderma species have been described.…”

Section: Introductionmentioning

confidence: 99%

Two new green-spored species of Trichoderma (Sordariomycetes, Ascomycota) and their phylogenetic positions

Zhu¹,

HaoXiang²,

Zhuang³

et al. 2017

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Two new species of Trichoderma are described based on the collections producing ascomata or asexual morphs on woody substrates, and named as Trichoderma fujianense and T. zonatum. Trichoderma fujianense produces gliocladium to verticillium-like conidiophores, slender to lageniform phialides, green and ellipsoidal to cylindrical conidia. Trichoderma zonatum is characterized by pulvinate, pale yellow to light brown stromata with densely disposed dark green to black ostioles, monomorphic ascospores, simple trichodermalike conidiophores, green, (sub)globose to pyriform conidia. Their phylogenetic positions were investigated inferred from sequence analyses of the combined RNA polymerase II subunit b and translation elongation factor 1-α genes. The results indicate that T. fujianense, along with T. aureoviride and T. candidum, represents an independent lineage with high statistical support. Trichoderma zonatum belongs to the Chlorosporum clade and is associated with but clearly separated from T. rosulatum and T. costaricense. Morphological distinctions and sequence divergences between the new species and their close relatives were discussed.

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“…The extraction was done with Wizard ® Genomic DNA Purification Kit (Promega, USA). The gene Elongation Factor (EF1α) was considered as the species marker [23,28–30]. …”

Section: Methodsmentioning

confidence: 99%

Recognition of endophyticTrichodermaspecies by leaf-cutting ants and their potential in a Trojan-horse management strategy

et al. 2017

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Interactions between leaf-cutting ants, their fungal symbiont (Leucoagaricus) and the endophytic fungi within the vegetation they carry into their colonies are still poorly understood. If endophytes antagonistic to Leucoagaricus were found in plant material being carried by these ants, then this might indicate a potential mechanism for plants to defend themselves from leaf-cutter attack. In addition, it could offer possibilities for the management of these important Neotropical pests. Here, we show that, for Atta sexdens rubropilosa, there was a significantly greater incidence of Trichoderma species in the vegetation removed from the nests—and deposited around the entrances—than in that being transported into the nests. In a no-choice test, Trichoderma-infested rice was taken into the nest, with deleterious effects on both the fungal gardens and ant survival. The endophytic ability of selected strains of Trichoderma was also confirmed, following their inoculation and subsequent reisolation from seedlings of eucalyptus. These results indicate that endophytic fungi which pose a threat to ant fungal gardens through their antagonistic traits, such as Trichoderma, have the potential to act as bodyguards of their plant hosts and thus might be employed in a Trojan-horse strategy to mitigate the negative impact of leaf-cutting ants in both agriculture and silviculture in the Neotropics. We posit that the ants would detect and evict such ‘malign’ endophytes—artificially inoculated into vulnerable crops—during the quality-control process within the nest, and, moreover, that the foraging ants may then be deterred from further harvesting of ‘Trichoderma-enriched’ plants.

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Unraveling Trichoderma species in the attine ant environment: description of three new taxa

Cited by 45 publications

References 64 publications

Fungal Identification Using Molecular Tools: A Primer for the Natural Products Research Community

Fungal Identification Using Molecular Tools: A Primer for the Natural Products Research Community

Two new green-spored species of Trichoderma (Sordariomycetes, Ascomycota) and their phylogenetic positions

Recognition of endophyticTrichodermaspecies by leaf-cutting ants and their potential in a Trojan-horse management strategy

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