Unraveling the Viral Complex Associated with La France Disease of the Cultivated Mushroom, Agaricus bisporus

Romaine, C. P.; Goodin, Michael M.

doi:10.1201/9781420039122.ch10

Cited by 15 publications

(28 citation statements)

References 72 publications

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“…Although most cause infections that appear innocuous, there are some well-known examples that dramatically alter the phenotype of their host (18). For example, a mycovirus of the tree pathogen Cryphonectria parasitica causes hypovirulence (23), a mycovirus of the edible mushroom Agaricus bisporus causes slow and aberrant growth (20), and a mycovirus of the endophyte Curvularia protuberata allows for the survival of the endophyte and the endophyte's host in geothermal soils (15).…”

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confidence: 99%

Aspergillus Mycoviruses Are Targets and Suppressors of RNA Silencing

et al. 2008

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RNA silencing can function as a virus defense mechanism in a diverse range of eukaryotes, and many viruses are capable of suppressing the silencing machinery targeting them. However, the extent to which this occurs between fungal RNA silencing and mycoviruses is unclear. Here, three Aspergillus dsRNA mycoviruses were partially characterized, and their relationship to RNA silencing was investigated. Aspergillus virus 1816 is related to Agaricus bisporus white button mushroom virus 1 and suppresses RNA silencing through a mechanism that alters the level of small interfering RNA. Aspergillus virus 178 is related to RNA virus L1 of Gremmeniella abietina and does not appear to affect RNA silencing. The third virus investigated, Aspergillus virus 341, is distantly related to Sphaeropsis sapinea RNA virus 2. Detection of mycovirus-derived siRNA from this mycovirus demonstrates that it is targeted for degradation by the Aspergillus RNA silencing machinery. Thus, our results indicate that Aspergillus mycoviruses are both targets and suppressors of RNA silencing. In addition, they suggest that the morphological and physiological changes associated with some mycoviruses could be a result of their antagonistic relationship with RNA silencing.

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confidence: 99%

Aspergillus Mycoviruses Are Targets and Suppressors of RNA Silencing

et al. 2008

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“…As Romaine et al (2002) have suggested future work requires a rigorous testing of cause and effect relationship; he also has raised a number of interesting propositions: are the dsRNAs exclusively associated with anomalies? Or will an exhaustive molecular search reveal the presence of dsRNAs at higher frequency in healthy tissues?…”

Section: Current Perspectives and Issues In Molecular Biology Of Mvxmentioning

confidence: 99%

“…An RT-PCR analysis estimated 60% of the diseased mushroom isolates in North America during a 13-year period were co-infected by LIV and MBV (Romaine et al, 1995) and that both can self replicate independently. MBV is unique, in that, it carries a positive sense ssRNA genome, a common feature of plant viruses (Tavantzis et al, 1980 and1983).…”

Section: Mvx In Great Britain (Gb) Uk -The Trials and Tribulations Omentioning

confidence: 99%

“…Among several current molecular techniques available, in terms of simplicity, versatility and applicability in a laboratory, the successful detection of known mushroom viruses (LIV or MBV) using RT-PCR methods (e.g. Romaine et al, 1995) and directly from composts (Sonnenberg et al, 1995) were regarded as attractive proposition and thus they served as templates for our development of rapid RT-PCR technique for viral detection. As a prequel to our eventual goal to detect MVX in composts, we initially tested the versatility of our version of RT-PCR protocol to monitor and successfully detect viruses in a separate study on waste composts laden with substantive mushroom mycelial residues.…”

Section: Direct Extraction Of Dsrnas From Mushroom Composts In Northementioning

confidence: 99%

“…In the last two decades, our understanding of the composition, genome organisation and replication of A. bisporus viruses, undoubtedly has been vastly advanced, but has raised further intriguing queries in the dsRNAs or viral complexes in A. bisporus which includes the prevalence of Hypoviridae-like dsRNA elements in healthy mushrooms and the frequent co-infection by single-stranded RNA (ssRNA) Mushroom bacilliform virus (MBV) alongside La France virus (Romaine and Goodin, 2002). Although the research reviewed herein deals in detail upon a modest selection of published data, intensive research upon MVX disease complex is being investigated by pioneers in mushroom molecular pathology (e.g.…”

Section: Introductionmentioning

confidence: 99%

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The Enigma of Double-stranded RNA (dsRNA) Associated with Mushroom Virus X (MVX)

Rao

Nelson²,

McClean³

2007

Current Issues in Molecular Biology

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New variants of pathogenic fungal viruses are emerging and they are enigmatic in revealing their molecular identity and of their origin. Double-stranded RNAs, some in non-encapsidated forms are increasingly becoming causal agents for sporadic diseases and are consistently associated with a complex profile of dsRNAs, presumably of (multiple) viral origin present in the host while the same are conspicuously absent in healthy (looking)

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The Ecology and Evolution of Fungal Viruses

Milgroom

Hillman

2011

Studies in Viral Ecology

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Unraveling the Viral Complex Associated with La France Disease of the Cultivated Mushroom, Agaricus bisporus

Cited by 15 publications

References 72 publications

Aspergillus Mycoviruses Are Targets and Suppressors of RNA Silencing

Aspergillus Mycoviruses Are Targets and Suppressors of RNA Silencing

The Enigma of Double-stranded RNA (dsRNA) Associated with Mushroom Virus X (MVX)

The Ecology and Evolution of Fungal Viruses

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