2019
DOI: 10.1074/jbc.ra119.007712
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Unraveling the subtleties of β-(1→3)-glucan phosphorylase specificity in the GH94, GH149, and GH161 glycoside hydrolase families

Abstract: Glycoside phosphorylases (GPs) catalyze the phosphorolysis of glycans into the corresponding sugar 1-phosphates and shortened glycan chains. Given the diversity of natural β-(1→3)-glucans and their wide range of biotechnological applications, the identification of enzymatic tools that can act on β-(1→3)-glucooligosaccharides is an attractive area of research. GP activities acting on β-(1→3)-glucooligosaccharides have been described in bacteria, the photosynthetic excavate Euglena gracilis … Show more

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Cited by 18 publications
(24 citation statements)
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“…oligosaccharides; no assessment of their donor substrate specificity has been reported to date. [7,15] In selecting sugar phosphates to assess with CDP and Pro_ 7066, we considered the synthesis of fragments of human milk oligosaccharides (HMOs), which have been the focus of recent enzymatic syntheses. [16] The synthesis of HMO-like molecules requires the ability to incorporate galactose, which is a prerequisite for later fucosylation or sialylation.…”
Section: Introductionmentioning
confidence: 99%
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“…oligosaccharides; no assessment of their donor substrate specificity has been reported to date. [7,15] In selecting sugar phosphates to assess with CDP and Pro_ 7066, we considered the synthesis of fragments of human milk oligosaccharides (HMOs), which have been the focus of recent enzymatic syntheses. [16] The synthesis of HMO-like molecules requires the ability to incorporate galactose, which is a prerequisite for later fucosylation or sialylation.…”
Section: Introductionmentioning
confidence: 99%
“…In terms of single sugar addition, as opposed to oligo/polymerisation reactions with its natural substrate Glc1P, CDP has been shown to be capable of using the anomeric phosphates of xylose (synthesis of a library of β‐(1,4) hetero‐oligosaccharides), galactose (biocatalytic production of novel glycolipids) and glucosamine (microscale reaction; preparative utility not demonstrated) . We recently identified algal and bacterial β‐1,3‐glucan phosphorylases (e.g., Pro_7066) that are capable of producing β‐1,3‐glucan oligosaccharides; no assessment of their donor substrate specificity has been reported to date …”
Section: Introductionmentioning
confidence: 99%
“…Thus, we were able to assign the enzymes suspected of being responsible for polysaccharide branching in Stramenopila to the GH16 subfamily 2. In addition, we checked a high number of putative beta-glucan candidate metabolic enzymes proposed in the literature for beta-glucan polysaccharide storage metabolism ( Michel et al, 2010 ; O’Neill et al, 2015 ; Kuhaudomlarp et al, 2019 ) and tried to correlate them with the presence of the GT48 and GH16 subfamily 2 ( Table 1 and Supplementary Table S1 ). Astonishingly, we did not find a correlation (absence in several Gyrista) with the GH16 subfamily 3 and 4 sequences despite these having been reported to encode laminarinase activities ( Viborg et al, 2019 ).…”
Section: Resultsmentioning
confidence: 99%
“…For CAZy families and subfamilies, we indicate with a white background if they were linked to one of the metabolisms. Despite a low correlation for GH161, we kept it because of its putative involvement as stated in Kuhaudomlarp et al (2019). Finally, we have indicated on the first column putative activities of each cazyme.…”
Section: Alpha-glucan Metabolism In Stramenopila Is From An Ancient Ementioning
confidence: 99%
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