Unraveling the serial glycosylation in the biosynthesis of steroidal saponins in the medicinal plant Paris polyphylla and their antifungal action

Chen, Yuegui; Yan, Qin; Ji, Yunheng; Bai, Xue; Li, Desen; Mu, Rong-Fang; Guo, Kai; Yang, Min-Jie; Yang, Tao; Gershenzon, Jonathan; Liu, Yan

doi:10.1016/j.apsb.2023.05.033

Cited by 3 publications

(1 citation statement)

References 56 publications

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“…UGT91AH1-3, derived from P. polyphylla in the Liliaceae family, is the only glycosyltransferase in the UGT91 family that can glycosylate the 6′- O -glucoside at the C-3 position of PPD-type ginsenosides to form ginsenoside 3β- O -Glc(1→6)-Rh 2 through the O-linkage chain (Table , Figure ). Furthermore, UGT91AH1-3 can utilize UDP-Glc, UDP-Rha, and UDP-Ara to generate the corresponding 6′- O -glucoside congeners using steroidal glycosides, diterpenoid glycosides, flavonoid glycosides, and other triterpenoid glycosides as substrates. This study provides an enzymatic tool for β-(1→6)-glycosylation of valuable terpenoid saponins.…”

Section: Introductionmentioning

confidence: 99%

Progress in Identification of UDP-Glycosyltransferases for Ginsenoside Biosynthesis

Yuan,

Li,

et al. 2024

J. Nat. Prod.

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Ginsenosides, the primary pharmacologically active constituents of the Panax genus, have demonstrated a variety of medicinal properties, including anticardiovascular disease, cytotoxic, antiaging, and antidiabetes effects. However, the low concentration of ginsenosides in plants and the challenges associated with their extraction impede the advancement and application of ginsenosides. Heterologous biosynthesis represents a promising strategy for the targeted production of these natural active compounds. As representative triterpenoids, the biosynthetic pathway of the aglycone skeletons of ginsenosides has been successfully decoded. While the sugar moiety is vital for the structural diversity and pharmacological activity of ginsenosides, the mining of uridine diphosphate-dependent glycosyltransferases (UGTs) involved in ginsenoside biosynthesis has attracted a lot of attention and made great progress in recent years. In this paper, we summarize the identification and functional study of UGTs responsible for ginsenoside synthesis in both plants, such as Panax ginseng and Gynostemma pentaphyllum, and microorganisms including Bacillus subtilis and Saccharomyces cerevisiae. The UGT-related microbial cell factories for large-scale ginsenoside production are also mentioned. Additionally, we delve into strategies for UGT mining, particularly potential rapid screening or identification methods, providing insights and prospects. This review provides insights into the study of other unknown glycosyltransferases as candidate genetic elements for the heterologous biosynthesis of rare ginsenosides.

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Section: Introductionmentioning

confidence: 99%

Progress in Identification of UDP-Glycosyltransferases for Ginsenoside Biosynthesis

Yuan,

Li,

et al. 2024

J. Nat. Prod.

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In vitro Evaluation of Fungal Susceptibility and Inhibition of Virulence by Diosgenin

do Socorro Costa,

da Silva,

Santos Araújo

et al. 2024

Chemistry & Biodiversity

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Fungal infections are a public health problem that mainly affects immunosuppressed people, Candida spp. have been responsible for most sources of contamination and invasive fungal infections described around the world. The need arises to find new therapeutic approaches to combat growing infections. Plants and natural products have been considered a valuable source for discovering new molecules with active ingredients. Diosgenin is a sapogenin found in the families of Leguminosae and Dioscoreaceae, it is obtained mainly from the dioscin saponin through the hydrolysis method, it is a phytochemical that has been highlighted in the treatment of various diseases, as well as in combating microbial resistance. The present study aimed to evaluate the susceptibility of fungal strains to diosgenin, as well as verify the association with the reference drug and evaluate the inhibition of the virulence factor through morphological changes in the yeast state to the filamentous form of hyphae and pseudohyphae in strains of Candida albicans, Candida tropicalis and Candida krusei using the broth microdilution method and microculture technique. Antifungal assays revealed that diosgenin was not able to inhibit the growth of the tested strains.

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Efficient extraction and optimization procedures of polyphyllins from Paris polyphylla var chinensis by deep eutectic solvent coupled with ultrasonic-assisted extraction

Tian,

Liu,

Jiang

et al. 2024

Microchemical Journal

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Unraveling the serial glycosylation in the biosynthesis of steroidal saponins in the medicinal plant Paris polyphylla and their antifungal action

Cited by 3 publications

References 56 publications

Progress in Identification of UDP-Glycosyltransferases for Ginsenoside Biosynthesis

Progress in Identification of UDP-Glycosyltransferases for Ginsenoside Biosynthesis

In vitro Evaluation of Fungal Susceptibility and Inhibition of Virulence by Diosgenin

Efficient extraction and optimization procedures of polyphyllins from Paris polyphylla var chinensis by deep eutectic solvent coupled with ultrasonic-assisted extraction

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