Unraveling the Role of the Apical Papilla During Dental Root Maturation

Driesen, Ronald B.; Gervois, Pascal; Vangansewinkel, Tim; Lambrichts, Ivo

doi:10.3389/fcell.2021.665600

Cited by 6 publications

(7 citation statements)

References 58 publications

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“…Y1 was sequenced using the 10X Genomics chromium platform, from which 12,620 cells were obtained. Y2 of GSM4365609 was in a later stage of development than Y1, and only the apical papilla of the root was extracted, which participates in the elongation and maturation of the root ( Driesen et al, 2021 ). In addition, two samples from mature tooth pulp (A1 and A2), for a total of four samples, were analyzed in our study to explore the spatial and temporal characterization of the gene expression in human tooth development ( Figure 1A ; Supplementary Table S1 ).…”

Section: Resultsmentioning

confidence: 99%

Atlas of human dental pulp cells at multiple spatial and temporal levels based on single-cell sequencing analysis

Ren¹,

Wen

Zhao³

et al. 2022

Front. Physiol.

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The dental pulp plays a crucial role in the long-term maintenance of tooth function. The progress of endodontic treatment and pulp tissue regeneration engineering has made pulp-regeneration therapy promising in clinical practice. However, the mechanisms of pulp regeneration and the role of dental stem cells in development and regeneration have not been fully elucidated. Bridging the gaps between clinical operation and basic research is urgently needed. With the application of single-cell sequencing technology in dental research, the landscapes of human dental pulp cells have begun being outlined. However, the specific cellular heterogeneity of dental pulp cells, especially that of dental stem cells, at different spatial and temporal levels, is still unclear. In this study, we used single-cell RNA sequencing analysis of pulp samples at four different developmental stages and combined the findings with immunohistochemical staining to explore the development of dental pulp and stem cells. The results revealed temporal changes in the proportion of pulp cells during development. For example, mononuclear phagocytes accounted for a higher proportion in early samples. Odontoblasts identified by DMP1 had a higher expression of ion channel-related and neurodevelopment-related genes. Subpopulations were identified in fibroblasts, odontoblasts, and mesenchymal stem cells. We identified a subclass of odontoblasts that expresses DGKI and RRBP1 present in early developmental samples. A population of earlier mesenchymal stem cells expressed the SEPTIN gene, which may have greater proliferative and differentiation potential. Furthermore, dental pulp stem cells can differentiate into two directions: mineralization and myogenesis. In summary, the specific cellular heterogeneity of dental pulp cells was revealed at different spatial and temporal levels. These findings may shed light on the mechanism of tooth development. The gene expression profile of developing pulp cells may help to select cells for regenerative engineering and improve the success of dental pulp regeneration.

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Section: Resultsmentioning

confidence: 99%

Atlas of human dental pulp cells at multiple spatial and temporal levels based on single-cell sequencing analysis

Ren¹,

Wen

Zhao³

et al. 2022

Front. Physiol.

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“…For example, SCAPs from teeth in the early root development stage showed better cell migration and osteogenic differentiation than those isolated from the tooth root at the late stage of development [18]. Third, the role of SCAPs in tooth root development is regulated by their in vivo niche, and in vitro culturing inevitably simplifies the regulation network SCAPs receive [19]. Therefore, different, even opposite, findings might be observed in studies from different research groups.…”

Section: Culture-dependent Methods and Scaps Studiesmentioning

confidence: 99%

Stem Cells from the Apical Papilla (SCAPs): Past, Present, Prospects, and Challenges

Liu,

Gao,

2023

Biomedicines

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Dental diseases occurring on young permanent teeth usually lead to the premature arrest of tooth root development. Sustained tooth root elongation is necessary to achieve the goal of long-term preservation of affected teeth. To this end, stem cell-based regenerative endodontic treatment has been regarded as one of the most promising strategies for treating young permanent teeth with pulp and periapical infections. Endogenous stem cells residing in the apical papilla, named stem cells from the apical papilla (SCAPs), have been intensively investigated due to their critical roles in pulp regeneration and root redevelopment. The present review summarizes advances in the field of SCAPs studies and discusses the challenges that need to be further addressed.

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“…To promote further root elongation and dentin formation, SCAPs can differentiate into odontoblasts. 70 The regulation of biological behaviors of SCAPs, such as differentiation, maturation, and mineralization, is tightly controlled by a cascade of signaling pathways, including TGF-β, WNT, and MAPK pathways (Fig. 3).…”

Section: Molecular Regulation Of Stem Cells From Apical Papilla Behav...mentioning

confidence: 99%

Spatiotemporal cellular dynamics and molecular regulation of tooth root ontogeny

Rao,

jing,

Fan

et al. 2023

Int J Oral Sci

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Tooth root development involves intricate spatiotemporal cellular dynamics and molecular regulation. The initiation of Hertwig’s epithelial root sheath (HERS) induces odontoblast differentiation and the subsequent radicular dentin deposition. Precisely controlled signaling pathways modulate the behaviors of HERS and the fates of dental mesenchymal stem cells (DMSCs). Disruptions in these pathways lead to defects in root development, such as shortened roots and furcation abnormalities. Advances in dental stem cells, biomaterials, and bioprinting show immense promise for bioengineered tooth root regeneration. However, replicating the developmental intricacies of odontogenesis has not been resolved in clinical treatment and remains a major challenge in this field. Ongoing research focusing on the mechanisms of root development, advanced biomaterials, and manufacturing techniques will enable next-generation biological root regeneration that restores the physiological structure and function of the tooth root. This review summarizes recent discoveries in the underlying mechanisms governing root ontogeny and discusses some recent key findings in developing of new biologically based dental therapies.

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Unraveling the Role of the Apical Papilla During Dental Root Maturation

Cited by 6 publications

References 58 publications

Atlas of human dental pulp cells at multiple spatial and temporal levels based on single-cell sequencing analysis

Atlas of human dental pulp cells at multiple spatial and temporal levels based on single-cell sequencing analysis

Stem Cells from the Apical Papilla (SCAPs): Past, Present, Prospects, and Challenges

Spatiotemporal cellular dynamics and molecular regulation of tooth root ontogeny

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