Unraveling the Role of Protein-Proteins Interactions of Annexin at the Membrane

Abstract

the wild-type protein, face the apolar environment of the lipid bilayer. We then measured the thermodynamic stability of the wild-type protein and of each of the sequence variants by chemical denaturation. We only made these measurements when the proteins appeared to be at reversible equilibrium between folded and denatured states. We also characterized the folded states of each protein by fluorescence spectroscopy and by a functional assay. Those characterizations revealed additional information about how the lipid bilayers may accommodate an arginine.