Unraveling the metabolic behavior in tomato high pigment mutants (hp-1, hp-2dg, og) and non ripening mutant (rin) during fruit ripening

Phenotypic Characterization of High Carotenoid Tomato Mutants Generated by the Target-AID Base-Editing Technology

“… Pal et al (2019) reported an increase in fructose for all mutants, whereas we observed a nonsignificant decrease in Slddb1-1 and Sldet1 , similar to what was observed by Yen et al (1997) and Enfissi et al (2010) in hp1 and hp2 , respectively ( Figure 6B ). For Slcyc-b mutant, similar observations by Pal et al (2019) with the og c mutant of an increase of the glucose and sucrose content were made ( Figures 6A , C ). However, we did not observe any changes in fructose contents ( Figure 6B ) compared to the increase in the og c mutant noticed by Pal et al (2019) .…”

“…The hp1 mutant shows a significant decrease in glucose and fructose with a higher increase in sucrose in red ripe fruits of Ailsa Craig ( Yen et al, 1997 ) according to Yen et al (1997) and a nonsignificant change in the accumulation of both sucrose and glucose associated with a decrease in fructose content was observed in several different hp2 mutants within different cultivated backgrounds ( Enfissi et al, 2010 ) according to Enfissi et al (2010) . However, another recent observation by Pal et al (2019) was in contradiction with these previous results, which noticed an increase in the glucose and fructose content associated with an increase in the sucrose content for all independent mutants hp1 , hp2 and og c . In the case of our single mutants Slddb1-1, Sldet1 and Slcyc-b in Micro-Tom, we observed a similar result by Pal et al (2019) concerning the glucose and sucrose content, with an increase in their accumulation ( Figure 6A ).…”

“…An investigation of sugar accumulation was conducted on ripe fruits to determine whether the mutations generated impacted the taste of the fruit. Previous analysis on cultivars showed a variable impact of the hp1 mutation on the sugar content ( Yen et al, 1997 ; Enfissi et al, 2010 ; Pal et al, 2019 ). Glucose tended to show higher accumulation in our single mutant lines ( Figure 6A ).…”

Characterization of a repertoire of tomato fruit genetic variants in the San marzano genetic background

“…Three single and two double mutants involved in fruit carotenoid accumulation showed a leaf CHL content lower than the WT, whereas two had higher leaf FLAV content, indicating that the variation in one class of pigments may significantly impact the levels of other classes. Apparently, a crosstalk among different metabolic pathways may be hypothesized and some findings seems to support this hypothesis (Minoggio et al, 2003;Pal et al, 2019). However, other evidences tend to limit the extent of the reciprocal influence between the phenylpropanoid/flavonoid and carotenoid pathways (Long et al, 2006), thus giving an input for deeper studies.…”

Inheritance of the Average Weight of the Fruit in Tomato Hybrids with High Lycopene Content

“…Highly pigmented forms with mutant genes hp-2 dg , B og , B c are a valuable material in the breeding for fruit quality, however, their characteristics are low productivity, late and extended ripening period, which significantly reduces the practical value [9][10][11][12]. Selection and involvement in crossing of early and high-yielding forms of a tomato allows to rececive a qualitative initial material which can be used in the further work for creation of heterosis hybrids and varieties [13][14].…”