Unraveling the Complexity of Liver Disease One Cell at a Time

Bader, Gary D.; McGilvray, Ian D.; MacParland, Sonya A.; Atif, Jawairia; Thoeni, Cornelia

doi:10.1055/s-0042-1755272

Cited by 4 publications

(1 citation statement)

References 180 publications

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“…To maintain liver homeostasis in a healthy state while remaining responsive to exogenous and endogenous stress, a highly organised and specialised structure of liver cells, including hormones, cytokines, metabolites, and microbial products, is needed to integrate local information at the molecular level. Liver research has embraced and benefited from innovative approaches (high-throughput single-cell technologies and multiomics), with several liver scRNA-seq studies being published in the past 5 years [ 17 – 23 ]. Recently, several reviews have summarised the application of single-cell techniques in various types of liver diseases, especially liver cancer [ 24 ], non-alcoholic fatty liver disease (NAFLD) [ 25 ], and fibrosis [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Liver in infections: a single-cell and spatial transcriptomics perspective

Zhang

Zhong

et al. 2023

J Biomed Sci

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The liver is an immune organ that plays a vital role in the detection, capture, and clearance of pathogens and foreign antigens that invade the human body. During acute and chronic infections, the liver transforms from a tolerant to an active immune state. The defence mechanism of the liver mainly depends on a complicated network of intrahepatic and translocated immune cells and non-immune cells. Therefore, a comprehensive liver cell atlas in both healthy and diseased states is needed for new therapeutic target development and disease intervention improvement. With the development of high-throughput single-cell technology, we can now decipher heterogeneity, differentiation, and intercellular communication at the single-cell level in sophisticated organs and complicated diseases. In this concise review, we aimed to summarise the advancement of emerging high-throughput single-cell technologies and re-define our understanding of liver function towards infections, including hepatitis B virus, hepatitis C virus, Plasmodium, schistosomiasis, endotoxemia, and corona virus disease 2019 (COVID-19). We also unravel previously unknown pathogenic pathways and disease mechanisms for the development of new therapeutic targets. As high-throughput single-cell technologies mature, their integration into spatial transcriptomics, multiomics, and clinical data analysis will aid in patient stratification and in developing effective treatment plans for patients with or without liver injury due to infectious diseases.

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Section: Introductionmentioning

confidence: 99%

Liver in infections: a single-cell and spatial transcriptomics perspective

Zhang

Zhong

et al. 2023

J Biomed Sci

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Single-cell, single-nucleus, and spatial transcriptomics characterization of the immunological landscape in the healthy and PSC human liver

Andrews,

Nakib,

Perciani

et al. 2024

Journal of Hepatology

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Single-cell and spatially resolved transcriptomics for liver biology

et al. 2023

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Single-cell transcriptomics enables the identification of rare cell types and the inference of state transitions, whereas spatially resolved transcriptomics allows the quantification of cells and genes in the context of tissues. The recent progress in these new technologies is improving our understanding of the cell landscape and its roles in diseases. Here, we review key biological insights into liver homeostasis, development, regeneration, chronic liver disease, and cancer obtained from single-cell and spatially resolved transcriptomics. We highlight recent progress in the liver cell atlas that characterizes the comprehensive cellular composition; diversity and function; the spatial architecture such as liver zonation, cell communication, and proximity; the cell identity conversion and cell-specific alterations that are associated with liver pathology; and new therapeutic targets. We further discuss outstanding challenges, advanced experimental technologies, and computational methods that help to address these challenges.

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Unraveling the Complexity of Liver Disease One Cell at a Time

Cited by 4 publications

References 180 publications

Liver in infections: a single-cell and spatial transcriptomics perspective

Liver in infections: a single-cell and spatial transcriptomics perspective

Single-cell, single-nucleus, and spatial transcriptomics characterization of the immunological landscape in the healthy and PSC human liver

Single-cell and spatially resolved transcriptomics for liver biology

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