Unraveling the anti-biofilm potential of green algal sulfated polysaccharides against Salmonella enterica and Vibrio harveyi

Vishwakarma, Jyoti; Vavilala, Sirisha L.

doi:10.1007/s00253-020-10653-5

Cited by 27 publications

(26 citation statements)

References 83 publications

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“…For example, Fucus species (Phaeophyceae) produce a variety of defensive secondary metabolites, such as phlorotannins and fucoxanthin, that likely represent selective filters for the bacteria that colonize these macroalgae [55][56][57][58]. Macroalgae also exude various polysaccharides that bacteria feed upon [59], and in some cases function as antibiotics to protect the host against pathogens or fouling organisms [60,61].…”

Section: Introductionmentioning

confidence: 99%

Alternative approaches to identify core bacteria in Fucus distichus microbiome and assess their distribution and host-specificity

Park

Davis

Lajoie

et al. 2022

Environmental Microbiome

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Background Identifying meaningful ecological associations between host and components of the microbiome is challenging. This is especially true for hosts such as marine macroalgae where the taxonomic composition of the microbiome is highly diverse and variable in space and time. Identifying core taxa is one way forward but there are many methods and thresholds in use. This study leverages a large dataset of microbial communities associated with the widespread brown macroalga, Fucus distichus, across sites and years on one island in British Columbia, Canada. We compare three different methodological approaches to identify core taxa at the amplicon sequence variant (ASV) level from this dataset: (1) frequency analysis of taxa on F. distichus performed over the whole dataset, (2) indicator species analysis (IndVal) over the whole dataset that identifies frequent taxa that are enriched on F. distichus in comparison to the local environment, and (3) a two-step IndVal method that identifies taxa that are consistently enriched on F. distichus across sites and time points. We then investigated a F. distichus time-series dataset to see if those core taxa are seasonally consistent on another remote island in British Columbia, Canada. We then evaluate host-specificity of the identified F. distichus core ASVs using comparative data from 32 other macroalgal species sampled at one of the sites. Results We show that a handful of core ASVs are consistently identified by both frequency analysis and IndVal approaches with alternative definitions, although no ASVs were always present on F. distichus and IndVal identified a diverse array of F. distichus indicator taxa across sites on Calvert Island in multiple years. Frequency analysis captured a broader suit of taxa, while IndVal was better at identifying host-specific microbes. Finally, two-step IndVal identified hundreds of indicator ASVs for particular sites/timepoints but only 12 that were indicators in a majority (> 6 out of 11) of sites/timepoints. Ten of these ASVs were also indicators on Quadra Island, 250 km away. Many F. distichus-core ASVs are generally found on multiple macroalgal species, while a few ASVs are highly specific to F. distichus. Conclusions Different methodological approaches with variable set thresholds influence core identification, but a handful of core taxa are apparently identifiable as they are widespread and temporally associated with F. distichus and enriched in comparison to the environment. Moreover, we show that many of these core ASVs of F. distichus are found on multiple macroalgal hosts, indicating that most occupy a macroalgal generalist niche rather than forming highly specialized associations with F. distichus. Further studies should test whether macroalgal generalists or specialists are more likely to engage in biologically important exchanges with host.

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Section: Introductionmentioning

confidence: 99%

Alternative approaches to identify core bacteria in Fucus distichus microbiome and assess their distribution and host-specificity

Park

Davis

Lajoie

et al. 2022

Environmental Microbiome

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“…Previous studies have shown that SCFAs play a key role in the regulation of host metabolism and intestinal inflammation ( Hernández et al, 2019 ; Xiao et al, 2020 ). Polysaccharides also significantly impact gut microbiota through the quorum sensing (QS) system ( Vishwakarma and Sirisha, 2020 ). Bacteria can release and exchange autoinducers (AIs), which monitor their population density and coordinate group behavior, including bacterial growth, proliferation, pathogenicity, and biofilm formation.…”

Section: Introductionmentioning

confidence: 99%

Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis

Yang

Meng

et al. 2021

Front. Microbiol.

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Polysaccharide is one of the main active ingredients of Polygonatum kingianum, which has been proven to regulate the balance of gut microbiota. For the first time, this study focused on the regulation of polysaccharides from Polygonatum kingianum (PS) on Lactobacillus faecis, a specific probiotic in the intestinal tract. PS effectively promoted the biomass, biofilm and acetic acid production in L. faecis 2-84, and enhanced quorum sensing (QS) signaling. The characteristics of gene sequence were analyzed using genomics approaches, and L. faecis 2-84 was found to encode 18 genes that are closely related to QS and 10 genes related to short-chain fatty acids (SCFAs). Additionally, transcriptome and proteome analysis demonstrated that PS could promote the QS system of L. faecis by enhancing the transcription of oppA gene and expression of oppD protein. PS also regulated the production and metabolism of SCFAs of L. faecis by upregulating the expression of ldh and metE gene and adh2 protein, and downregulating the expression of mvK gene. In conclusion, it was speculated that PS could affect intestinal SCFAs production by affecting the QS system and SCFAs production in L. faecis. The present study implied that PS might have a role in promoting the growth of intestinal probiotics, where the QS system and SCFAs might be two of the important mechanisms for the probiotic activity of PS.

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“…SPs extracted from C. reinhardtii also showed concentration-dependent increase in the percentage inhibition of biofilm formation against food-borne infections causing bacteria [72]. The probable mode of action of these polysaccharides could be their ability to modify the physical characteristics of bacterial cells, thereby inhibiting them to bind to surfaces, or they might modify the gene expression of recipient bacteria by acting as signalling molecules, or they can also have competitive inhibition of carbohydrate-protein interactions with the bacterial surface, thereby altering the stability of EPS layer [71,[73][74][75][76]. However the exact mode of action of Cr-SPs needs to be explored in future.…”

Section: Discussionmentioning

confidence: 99%

Algal polysaccharide’s potential to combat respiratory infections caused by Klebsiella pneumoniae and Serratia marcescens biofilms

Vishwakarma

Waghela

Falcao

et al. 2021

Appl Biochem Biotechnol

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The growth of respiratory diseases, as witnessed through the SARS and COVID-19 outbreaks, and antimicrobial-resistance together pose a serious threat to humanity. One reason for antimicrobial resistance is formation of bacterial biofilms. In this study the sulphated polysaccharides from green algae Chlamydomonas reinhardtii (Cr-SPs) is tested for its antibacterial and antibiofilm potential against Klebsiella pneumoniae and Serratia marcescens. Agar cup assay clearly indicated the antibacterial potential of Cr-SPs. Minimum inhibitory concentration (MIC 50 ) of Cr-SPs against Klebsiella pneumoniae was found to be 850 µg/ml, and it is 800 µg/ml in Serratia marcescens. Time-kill and colony-forming ability assays suggest the concentration-dependent bactericidal potential of Cr-SPs. Cr-SPs showed 74-100% decrease in biofilm formation in a concentration-dependent manner by modifying the cell surface hydrophobic properties of these bacteria. Cr-SPs have also distorted preformed-biofilms by their ability to interact and destroy the extra polymeric substance and eDNA of the matured biofilm. Scanning electron microscopy analysis showed that Cr-SPs effectively altered the morphology of these bacterial cells and distorted the bacterial biofilms. Furthermore reduced protease, urease and prodigiosin pigment production suggest that Cr-SPs interferes the quorum sensing mechanism in these bacteria. The current study paves way towards developing Cr-SPs as a control strategy for treatment of respiratory tract infections.

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Unraveling the anti-biofilm potential of green algal sulfated polysaccharides against Salmonella enterica and Vibrio harveyi

Cited by 27 publications

References 83 publications

Alternative approaches to identify core bacteria in Fucus distichus microbiome and assess their distribution and host-specificity

Alternative approaches to identify core bacteria in Fucus distichus microbiome and assess their distribution and host-specificity

Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis

Algal polysaccharide’s potential to combat respiratory infections caused by Klebsiella pneumoniae and Serratia marcescens biofilms

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