Unraveling Regulation of the Small Heat Shock Proteins by the Heat Shock Factor HvHsfB2c in Barley: Its Implications in Drought Stress Response and Seed Development

Reddy, Palakolanu Sudhakar; Kishor, P. B. Kavi; Seiler, Christiane; Kuhlmann, Markus; Eschen‐Lippold, Lennart; Lee, Justin; Reddy, Malireddy K.; Sreenivasulu, Nese

doi:10.1371/journal.pone.0089125

Cited by 89 publications

(64 citation statements)

References 64 publications

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“…5,D and E). This indicated that Hsp16.9 and Hsp17.5 are cytosolic proteins, as predicted by Reddy et al (2014), and that their interactions with CSEP0105 and CSEP0162 likely occur in the cytosol. effect of their interactions on the localizations of the CSEPs and Hsps.…”

Section: Csep0105 and Csep0162 Are Highly Expressed During And After mentioning

confidence: 76%

“…HvHsp16.9-CI; GenBank accession no. AK362925; Reddy et al, 2014). The identified cDNA has 14 nucleotide substitutions compared with HvHsp16.9-CI, where 12 are synonymous and two are nonsynonymous (Supplemental Fig.…”

Section: Csep0105 and Csep0162 Are Highly Expressed During And After mentioning

confidence: 99%

“…Using a genome-wide sequence survey, Reddy et al (2014) discovered 18 full-length sHsps that are differentially regulated during drought stress and seed development in barley. We searched and identified 19 additional full-length sHsps (Supplemental Table S1) in the recently published barley genome (International Barley Genome Sequencing Consortium, 2012) and the full-length barley cDNAs at the National Center for Biotechnology Information (NCBI; Matsumoto et al, 2011).…”

Section: Csep0105 and Csep0162 Are Highly Expressed During And After mentioning

confidence: 99%

“…The two sHsps identified in the Y2H screen, RSI2, Nthsp17, Nbhsp17, and Mds1 are indicated by arrows. The naming follows Reddy et al (2014) for those starting with HvHsp. The MLOC names are from the annotation of the barley genome.…”

Section: Csep0105 and Csep0162 Are Highly Expressed During And After mentioning

confidence: 99%

“…To identify sHsps in barley, we used the 19 barley sHsps described by Reddy et al (2014) and rice (Oryza sativa) sHsps obtained from the Phytozome server (http://www.phytozome.net/) as queries in BLASTp searches of the high-confidence barley genome sequence predicted by the International Barley Genome Sequencing Consortium (2012; http://mips.helmholtz-muenchen. de/plant/barley/download/index.jsp) and of barley ESTs at NCBI, HarvEST (Close et al, 2004), and PLEXdb (Dash et al, 2012).…”

Section: Identification Of Shsps In the Barley Genome And Clustering mentioning

confidence: 99%

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The Barley Powdery Mildew Candidate Secreted Effector Protein CSEP0105 Inhibits the Chaperone Activity of a Small Heat Shock Protein

et al. 2015

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Pathogens secrete effector proteins to establish a successful interaction with their host. Here, we describe two barley (Hordeum vulgare) powdery mildew candidate secreted effector proteins, CSEP0105 and CSEP0162, which contribute to pathogen success and appear to be required during or after haustorial formation. Silencing of either CSEP using host-induced gene silencing significantly reduced the fungal haustorial formation rate. Interestingly, both CSEPs interact with the barley small heat shock proteins, Hsp16.9 and Hsp17.5, in a yeast two-hybrid assay. Small heat shock proteins are known to stabilize several intracellular proteins, including defense-related signaling components, through their chaperone activity. CSEP0105 and CSEP0162 localized to the cytosol and the nucleus of barley epidermal cells, whereas Hsp16.9 and Hsp17.5 are cytosolic. Intriguingly, only those specific CSEPs changed localization and became restricted to the cytosol when coexpressed with Hsp16.9 and Hsp17.5, confirming the CSEP-small heat shock protein interaction. As predicted, Hsp16.9 showed chaperone activity, as it could prevent the aggregation of Escherichia coli proteins during thermal stress. Remarkably, CSEP0105 compromised this activity. These data suggest that CSEP0105 promotes virulence by interfering with the chaperone activity of a barley small heat shock protein essential for defense and stress responses.

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Section: Csep0105 and Csep0162 Are Highly Expressed During And After mentioning

confidence: 76%

Section: Csep0105 and Csep0162 Are Highly Expressed During And After mentioning

confidence: 99%

Section: Csep0105 and Csep0162 Are Highly Expressed During And After mentioning

confidence: 99%

Section: Csep0105 and Csep0162 Are Highly Expressed During And After mentioning

confidence: 99%

Section: Identification Of Shsps In the Barley Genome And Clustering mentioning

confidence: 99%

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The Barley Powdery Mildew Candidate Secreted Effector Protein CSEP0105 Inhibits the Chaperone Activity of a Small Heat Shock Protein

et al. 2015

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Plant Molecular Chaperones: Structural Organization and their Roles in Abiotic Stress Tolerance

Singh

Gupta

Prasad

2019

Molecular Plant Abiotic Stress

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Specific adaptation for early maturity and height stability in Icelandic spring barley

et al. 2021

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Cereal production in important growing regions is negatively influenced by climate change.This can be countered by expanding cereal production northwards in Scandinavia and Iceland, where today barley is primarily used as feed, rarely reaching malting quality. This study explores genetic factors underlying the ability of barley to mature fully in low temperature and long photoperiod. A panel of 84 spring barley lines were grown in controlled environments with different day lengths and temperatures, partially mimicking the target environment. The panel was screened for accumulated heat sum to heading and maturity, and height; traits of importance for adaptation to the northern periphery. Subgroups with different stability and heat sum requirements were found, and day length neutral lines were identified.Height was temperature controlled, with lower temperature resulting in taller plants. The results were coupled to a genome wide association study (GWAS). Despite the small panel size, the Mat-a locus was identified to have the strongest association with heat sum to heading, Ppd-H1, Mat-a, FT1 and DHAR2 with heat sum to maturity, and GA20ox1 with height. Early maturing lines with height stability have successfully been developed in Iceland and this study confirms their performance in controlled environments for the first time. It provides insight to the mechanisms behind early maturity that will increase our ability to further adapt barley, and other cereals, to the northern climate. This will facilitate breeding work towards combining early maturity and height stability with traits such as quality, further enabling the northward expansion of grain production.

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Unraveling Regulation of the Small Heat Shock Proteins by the Heat Shock Factor HvHsfB2c in Barley: Its Implications in Drought Stress Response and Seed Development

Cited by 89 publications

References 64 publications

The Barley Powdery Mildew Candidate Secreted Effector Protein CSEP0105 Inhibits the Chaperone Activity of a Small Heat Shock Protein

The Barley Powdery Mildew Candidate Secreted Effector Protein CSEP0105 Inhibits the Chaperone Activity of a Small Heat Shock Protein

Plant Molecular Chaperones: Structural Organization and their Roles in Abiotic Stress Tolerance

Specific adaptation for early maturity and height stability in Icelandic spring barley

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