Unraveling nitrogen, sulfur and carbon metabolic pathways and microbial community transcriptional responses to substrate deprivation and toxicity stresses in a bioreactor mimicking anoxic brackish coastal sediment conditions
Abstract:Microbial communities are key drivers of carbon, sulfur and nitrogen cycling in coastal ecosystems, where they are subjected to dynamic shifts in substrate availability and exposure to toxic compounds. However, how these shifts affect microbial interactions and function is poorly understood. Unraveling such microbial community responses is key to understand their environmental distribution and resilience under current and future disturbances. Here, we used metagenomics and metatranscriptomics to investigate mi… Show more
“…These 12 samples were sequenced by Macrogen Europe BV (Amsterdam, Netherlands) using the TruSeq Nano DNA library with an insert size of 350bp on an Illumina NovaSeq6000 platform, producing 2 × 151 bp paired-end reads. Reads were processed to generate metagenomeassembled genomes (MAGs) as previously described 49 and as detailed in the supplement. MAGs were taxonomically classified with GTDB-Tk v1 50 and annotated with DRAM v1.0.…”
“…These 12 samples were sequenced by Macrogen Europe BV (Amsterdam, Netherlands) using the TruSeq Nano DNA library with an insert size of 350bp on an Illumina NovaSeq6000 platform, producing 2 × 151 bp paired-end reads. Reads were processed to generate metagenomeassembled genomes (MAGs) as previously described 49 and as detailed in the supplement. MAGs were taxonomically classified with GTDB-Tk v1 50 and annotated with DRAM v1.0.…”
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