Unraveling Nitrogen, Sulfur, and Carbon Metabolic Pathways and Microbial Community Transcriptional Responses to Substrate Deprivation and Toxicity Stresses in a Bioreactor Mimicking Anoxic Brackish Coastal Sediment Conditions
Abstract:Microbial communities are key drivers of carbon, sulfur, and nitrogen cycling in coastal ecosystems, where they are subjected to dynamic shifts in substrate availability and exposure to toxic compounds. However, how these shifts affect microbial interactions and function is poorly understood. Unraveling such microbial community responses is key to understand their environmental distribution and resilience under current and future disturbances. Here, we used metagenomics and metatranscriptomics to investigate m… Show more
“…These 12 samples were sequenced by Macrogen Europe BV (Amsterdam, Netherlands) using the TruSeq Nano DNA library with an insert size of 350bp on an Illumina NovaSeq6000 platform, producing 2 × 151 bp paired-end reads. Reads were processed to generate metagenome-assembled genomes (MAGs) as previously described and as detailed in the supplement. MAGs were taxonomically classified with GTDB-Tk v1 and annotated with DRAM v1.0 .…”
“…These 12 samples were sequenced by Macrogen Europe BV (Amsterdam, Netherlands) using the TruSeq Nano DNA library with an insert size of 350bp on an Illumina NovaSeq6000 platform, producing 2 × 151 bp paired-end reads. Reads were processed to generate metagenome-assembled genomes (MAGs) as previously described and as detailed in the supplement. MAGs were taxonomically classified with GTDB-Tk v1 and annotated with DRAM v1.0 .…”
“…Synergistic or antagonistic effects of C, N, and S. Interactions among carbon, nitrogen, and sulfur in microbial ecosystems, especially under low-concentration conditions, is critical for assessing their synergistic and antagonistic effects on microbial metabolism 61 . These interactions can significantly influence the efficiency of microbial concentrating mechanisms and the overall biogeochemical cycles in nutrient-limited environments 62 .…”
Microbial metabolism upholds a fundamental role in the sustainability of water ecosystems. However, how microorganisms surviving in low-concentration substrate water environments, including the existence of emerging compounds of interest, remains unclear. In this review, microbial strategies for concentrating, utilizing, and metabolizing of low concentration substrates were summarized. Microorganisms develop substrate-concentrating strategies at both the cell and aggregate levels in substrate-limited settings. Following, microbial uptake and transport of low-concentration substrates are facilitated by adjusting physiological characteristics and shifting substrate affinities. Finally, metabolic pathways, such as mixed-substrate utilization, syntrophic metabolism, dynamic response to nutrient variation, and population density-based mechanisms allow microorganisms to efficiently utilize low-concentration substrates and to adapt to challenging oligotrophic environments. All these microbial strategies will underpin devising new approaches to tackle environmental challenges and drive the sustainability of water ecosystems, particularly in managing low-concentration contaminants (i.e., micropollutants).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.