Unraveling mosquito metabolism with mass spectrometry-based metabolomics

Horvath, Thomas D.; Dagan, S.; Scaraffia, Patricia Y.

doi:10.1016/j.pt.2021.03.010

Cited by 11 publications

(6 citation statements)

References 67 publications

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“…In previous studies, the total carbohydrates of the mosquito diets were analysed via cold anthrone test [ [22] , [23] , [24] ], whereas proteins, lipids, and other nutrients also including carbohydrates were analysed by gas chromatography - mass spectrometry (GC-MS) through a derivatization process [ [25] , [26] , [27] , [28] ]. In other studies, a number of environmental pollutants such as pesticides were detected in the crop and midgut of the mosquitoes [ 29 ]; as well as in fruits and plants [ 30 , 31 ] by liquid chromatography tandem mass spectrometry (LC-MS/MS). Considering the studies mentioned above and the simplest and most convenient method for sample preparation [ 32 ], LC-MS is a most appropriate technique for determining altogether sugars and other chemical compounds in mosquito’ crops.…”

Section: Discussionmentioning

confidence: 99%

Unveil the sugar diet and associated environmental compounds in the crop of the mosquito Culex pipiens

Leyva,

Brustolin,

Müller

et al. 2024

Heliyon

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Section: Discussionmentioning

confidence: 99%

Unveil the sugar diet and associated environmental compounds in the crop of the mosquito Culex pipiens

Leyva,

Brustolin,

Müller

et al. 2024

Heliyon

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“…This is reflective of dependence on de novo fatty acid synthesis to fulfill lipid requirements that are not fulfilled by the high sugar diet (Jenkin et al, 1975;Sushchik et al, 2013;Chotiwan et al, 2022) (F) Utilization of amino acids in the blood meal by female mosquitoes. A major proportion of the blood meal amino acids are oxidized to CO 2 or excreted as uric acid while the remaining portion is converted to triglycerides (TAGs) and stored in the mosquito fat body (Lehane, 1991;Zhou et al, 2004a;Horvath et al, 2021). Deposited TAGs are used in oogenesis, metamorphosis, prolonged flight and other physiological processes.…”

Section: Metabolism Is At the Forefront Of Mosquito Development And B...mentioning

confidence: 99%

“…generated during the oxidation of amino acids in a blood meal, mosquitoes were fed with labeled 15 NH 4 Cl. The labeled 15 N was traced in the whole mosquito body using electrospray ionization (ESI)-mass spectrometry and stable label isotope tracing (Horvath et al, 2021). The study showed that 15 N was rapidly incorporated into glutamine (Gln) via glutamine synthase (GS) and with the aid of other enzymes, additional N-containing metabolites were generated in the mosquito (Horvath et al, 2021).…”

Section: Adult Mosquitoesmentioning

confidence: 99%

“…The labeled 15 N was traced in the whole mosquito body using electrospray ionization (ESI)-mass spectrometry and stable label isotope tracing (Horvath et al, 2021). The study showed that 15 N was rapidly incorporated into glutamine (Gln) via glutamine synthase (GS) and with the aid of other enzymes, additional N-containing metabolites were generated in the mosquito (Horvath et al, 2021). However, 16% of the meal was converted to TAG, the storage lipid (Zhou et al, 2004a).…”

Section: Adult Mosquitoesmentioning

confidence: 99%

See 1 more Smart Citation

The buzz in the field: the interaction between viruses, mosquitoes, and metabolism

Ratnayake

Chotiwan

Saavedra-Rodríguez

et al. 2023

Front. Cell. Infect. Microbiol.

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Among many medically important pathogens, arboviruses like dengue, Zika and chikungunya cause severe health and economic burdens especially in developing countries. These viruses are primarily vectored by mosquitoes. Having surmounted geographical barriers and threat of control strategies, these vectors continue to conquer many areas of the globe exposing more than half of the world’s population to these viruses. Unfortunately, no medical interventions have been capable so far to produce successful vaccines or antivirals against many of these viruses. Thus, vector control remains the fundamental strategy to prevent disease transmission. The long-established understanding regarding the replication of these viruses is that they reshape both human and mosquito host cellular membranes upon infection for their replicative benefit. This leads to or is a result of significant alterations in lipid metabolism. Metabolism involves complex chemical reactions in the body that are essential for general physiological functions and survival of an organism. Finely tuned metabolic homeostases are maintained in healthy organisms. However, a simple stimulus like a viral infection can alter this homeostatic landscape driving considerable phenotypic change. Better comprehension of these mechanisms can serve as innovative control strategies against these vectors and viruses. Here, we review the metabolic basis of fundamental mosquito biology and virus-vector interactions. The cited work provides compelling evidence that targeting metabolism can be a paradigm shift and provide potent tools for vector control as well as tools to answer many unresolved questions and gaps in the field of arbovirology.

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“…In the last decade, GC-MS methods have been applied to address effects of stresses, including heat, freezing, and desiccation (Michaud and Denlinger, 2007;Robert Michaud et al, 2008;Koštál et al, 2011;Colinet et al, 2012;Mamai et al, 2014;Cox et al, 2017;Horvath et al, 2021). Nanospray ionization tandem mass spectrometry approaches have been used to investigate ammonia and nitrogen metabolism in Aedes aegypti (Scaraffia et al, 2006(Scaraffia et al, , 2008, as well as the changes in metabolomic profiles in response to different diets and after pathogen infection in Anopheles (Champion et al, 2017).…”

Section: Initial Studies Of Insect Metabolomesmentioning

confidence: 99%

Beyond Sperm and Male Accessory Gland Proteins: Exploring Insect Reproductive Metabolomes

2021

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Insect seminal fluid, the non-sperm component of the ejaculate, comprises a variegated set of molecules, including, but not limited to, lipids, proteins, carbohydrates, salts, hormones, nucleic acids, and vitamins. The identity and functional role of seminal fluid proteins (SFPs) have been widely investigated, in multiple species. However, most of the other small molecules in insect ejaculates remain uncharacterized. Metabolomics is currently adopted to deepen our understanding of complex biological processes and in the last 15years has been applied to answer different physiological questions. Technological advances in high-throughput methods for metabolite identification such as mass spectrometry and nuclear magnetic resonance (NMR) are now coupled to an expanded bioinformatics toolbox for large-scale data analysis. These improvements allow for the processing of smaller-sized samples and for the identification of hundreds to thousands of metabolites, not only in Drosophila melanogaster but also in disease vectors, animal, and agricultural pests. In this review, we provide an overview of the studies that adopted metabolomics-based approaches in insects, with a particular focus on the reproductive tract (RT) of both sexes and the ejaculate. Progress in the field of metabolomics will contribute not only to achieve a deeper understanding of the composition of insect ejaculates and how they are affected by endogenous and exogenous factors, but also to provide increasingly powerful tools to decipher the identity and molecular interactions between males and females during and after mating.

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Unraveling mosquito metabolism with mass spectrometry-based metabolomics

Cited by 11 publications

References 67 publications

Unveil the sugar diet and associated environmental compounds in the crop of the mosquito Culex pipiens

Unveil the sugar diet and associated environmental compounds in the crop of the mosquito Culex pipiens

The buzz in the field: the interaction between viruses, mosquitoes, and metabolism

Beyond Sperm and Male Accessory Gland Proteins: Exploring Insect Reproductive Metabolomes

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