Unraveling mechanisms of cell expansion linking solute transport, metabolism, plasmodesmtal gating and cell wall dynamics

Wang, Lu; Ruan, Yong‐Ling

doi:10.4161/psb.5.12.13568

Cited by 31 publications

(40 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cotton fibre elongates through diffuse growth mechanism and turgor-driven pressure is a significant driving force for cell enlargement [17,18,53,54]. The turgor-driven pressure largely depends on accumulation of osmolytes and ion-homeostasis [17,18].…”

Section: Resultsmentioning

confidence: 99%

“…The turgor-driven pressure largely depends on accumulation of osmolytes and ion-homeostasis [17,18]. Further, genes involved in ROS homeostasis and protein stabilization play a crucial role during fibre development [6,8].…”

Section: Resultsmentioning

confidence: 99%

“…Phytohormones such as ethylene [9], auxins [10,11] and brassinosteroids (BR) [12,13] and transcription factors such as MYB25 [14] and MYB25-like [15,16] were shown to be involved in fibre development. Cotton fibre elongates by diffusion growth mechanism and the turgor driven force is required for unidirectional elongation [17,18]. Therefore, osmotically active solutes (soluble sugars, potassium and malate) and ion-transporters (H + -ATPases and K + -transporter) play an important role in maintaining the osmotic potential of the elongating fibre cell [18].…”

Section: Introductionmentioning

confidence: 99%

“…Cotton fibre elongates by diffusion growth mechanism and the turgor driven force is required for unidirectional elongation [17,18]. Therefore, osmotically active solutes (soluble sugars, potassium and malate) and ion-transporters (H + -ATPases and K + -transporter) play an important role in maintaining the osmotic potential of the elongating fibre cell [18]. It is reported that the closure of plasmodesmata (PD) and the coordinated up-regulation of potassium (K + ) and sugar transporters during fibre elongation stage maintains the turgor pressure required for the fibre cell elongation and the duration of PD closure correlates positively with the fibre length [19].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Functional genomics of fuzzless-lintless mutant of Gossypium hirsutum L. cv. MCU5 reveal key genes and pathways involved in cotton fibre initiation and elongation

et al. 2012

View full text Add to dashboard Cite

BackgroundFuzzless-lintless cotton mutants are considered to be the ideal material to understand the molecular mechanisms involved in fibre cell development. Although there are few reports on transcriptome and proteome analyses in cotton at fibre initiation and elongation stages, there is no comprehensive comparative transcriptome analysis of fibre-bearing and fuzzless-lintless cotton ovules covering fibre initiation to secondary cell wall (SCW) synthesis stages. In the present study, a comparative transcriptome analysis was carried out using G. hirsutum L. cv. MCU5 wild-type (WT) and it’s near isogenic fuzzless-lintless (fl) mutant at fibre initiation (0 dpa/days post anthesis), elongation (5, 10 and 15 dpa) and SCW synthesis (20 dpa) stages.ResultsScanning electron microscopy study revealed the delay in the initiation of fibre cells and lack of any further development after 2 dpa in the fl mutant. Transcriptome analysis showed major down regulation of transcripts (90%) at fibre initiation and early elongation (5 dpa) stages in the fl mutant. Majority of the down regulated transcripts at fibre initiation stage in the fl mutant represent calcium and phytohormone mediated signal transduction pathways, biosynthesis of auxin and ethylene and stress responsive transcription factors (TFs). Further, transcripts involved in carbohydrate and lipid metabolisms, mitochondrial electron transport system (mETS) and cell wall loosening and elongation were highly down-regulated at fibre elongation stage (5–15 dpa) in the fl mutant. In addition, cellulose synthases and sucrose synthase C were down-regulated at SCW biosynthesis stage (15–20 dpa). Interestingly, some of the transcripts (~50%) involved in phytohormone signalling and stress responsive transcription factors that were up-regulated at fibre initiation stage in the WT were found to be up-regulated at much later stage (15 dpa) in fl mutant.ConclusionsComparative transcriptome analysis of WT and its near isogenic fl mutant revealed key genes and pathways involved at various stages of fibre development. Our data implicated the significant role of mitochondria mediated energy metabolism during fibre elongation process. The delayed expression of genes involved in phytohormone signalling and stress responsive TFs in the fl mutant suggests the need for a coordinated expression of regulatory mechanisms in fibre cell initiation and differentiation.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Functional genomics of fuzzless-lintless mutant of Gossypium hirsutum L. cv. MCU5 reveal key genes and pathways involved in cotton fibre initiation and elongation

et al. 2012

View full text Add to dashboard Cite

show abstract

“…GhEXP1, which is related to fiber elongation and specific expression during fiber elongation, is a member of the Expansin gene family [7]. Sucrose synthase can catalyze the transformation of sucrose into fructose and uridine diphosphate glucose (UDPG), which can increase intracellular osmotic stress and promote the initiation of ovule epidermal cells [8]. Furthermore, UDPG can be transported to the cellulose synthase catalytic subunit and is mainly used for the biosynthesis of cellulose [9].…”

Section: Digital Signaturementioning

confidence: 99%

Effects of light on in vitro fiber development and flavonoid biosynthesis in green cotton (Gossypium hirsutum)

et al. 2016

View full text Add to dashboard Cite

As an environmental factor, light influences the physiological functions and secondary metabolism of plants. However, the role of light in cotton fiber development and pigment biosynthesis has not yet been thoroughly explored. In this study, ovules of green cotton were cultured in vitro under dark and light conditions, and fiber and ovule growth parameters as well as fiber carbohydrate and cellulose contents and the expression of genes related to fiber development were investigated to elucidate the effect of light on fiber development. In addition, to investigate the effect of light on fiber pigment biosynthesis, the fiber flavonoid content and related gene expression were determined. The results demonstrated that the fiber length and the expression levels of fiber elongation genes under light culture were significantly lower than under dark culture, however, the ovule and fiber weight were significantly higher than under dark culture. The fiber developed under light culture had higher carbohydrate concentrations and carbohydrate transformation rate than under dark culture. Additionally, light culture exhibited higher cellulose contents and expression levels of cellulose biosynthesis genes compared with dark culture. In contrast, the pattern of the effect of light on flavonoid biosynthesis differed from that for cellulose biosynthesis. At 10 DAC (days after culture) and 20 DAC, the flavonoid contents and the expression levels of genes related to flavonoid biosynthesis were lower than under dark culture. However, the flavonoid contents and gene expression levels observed at 30 DAC and 40 DAC were higher in the light culture than in the dark culture. These results suggested that light hindered fiber elongation, but promoted carbohydrate accumulation and carbohydrate transformation, which resulted in fiber weight gain and increased cellulose accumulation in fibers. In addition, light inhibited flavonoid biosynthesis at early stage of fiber development, but promoted it at later stages. These findings provide the basis for intensive study of fiber development and flavonoids biosynthesis in green cotton.

show abstract

QTL mapping and candidate gene identification of lint percentage based on a recombinant inbred line population of upland cotton

et al. 2021

View full text Add to dashboard Cite

Unraveling mechanisms of cell expansion linking solute transport, metabolism, plasmodesmtal gating and cell wall dynamics

Cited by 31 publications

References 19 publications

Functional genomics of fuzzless-lintless mutant of Gossypium hirsutum L. cv. MCU5 reveal key genes and pathways involved in cotton fibre initiation and elongation

Functional genomics of fuzzless-lintless mutant of Gossypium hirsutum L. cv. MCU5 reveal key genes and pathways involved in cotton fibre initiation and elongation

Effects of light on in vitro fiber development and flavonoid biosynthesis in green cotton (Gossypium hirsutum)

QTL mapping and candidate gene identification of lint percentage based on a recombinant inbred line population of upland cotton

Contact Info

Product

Resources

About