Unraveling Low-Level Gamma Radiation-Responsive Changes in Expression of Early and Late Genes in Leaves of Rice Seedlings at litate Village, Fukushima

Hayashi, Gosuke; Shibato, Junko; Imanaka, Tetsuji; Cho, Kyoungwon; Kubo, Akihiro; Kikuchi, Shoshi; Satoh, Kouji; Kimura, Masayoshi; Ozawa, Shoji; Fukutani, Satoshi; Endo, Satoru; Ichikawa, Katsuki; Agrawal, Ganesh Kumar; Shioda, Seiji; Fukumoto, Manabu; Rakwal, Randeep

doi:10.1093/jhered/esu025

Cited by 41 publications

(37 citation statements)

References 38 publications

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“…The results of this analysis complement the ones previously obtained in the context of genome and transcriptome. 13 by adding a proteome dimension as well.…”

Section: Discussionmentioning

confidence: 99%

“…We could speculate that the considerable reduction in expression of photosynthesis proteins could be linked with the withering observed at the tips of the irradiated leaves. 13 …”

Section: Energymentioning

confidence: 99%

“…Results presented here provide not only a support to but also confirm our previous researches conducted genome-wide, in the laboratory using cut rice leaf segments (invitro experiments), which revealed ultra-/ low-level gamma radiation induced selfdefense responses in rice. 3,4 Secondly, the current research complements the first genome-wide data 13 on rice plants response to low-level gamma radiation in a radioactively contaminated field environment by presenting new proteome-wide analysis data.…”

mentioning

confidence: 98%

“…12 The experiment was performed in the summer of 2012, where the rice plants were exposed to low-level gamma radiation, being emitted from the contaminated ground without causing internal contamination, followed by an investigation into the morphological and molecular genetic changes in leaves under a dose-dependent manner. 13 In that study, gamma radiation-exposed samples were examined by RT-PCR to reveal the differential gene expression in leaves in a time-dependent manner over 3 days. Further, the genomewide transcriptome profiling of changed genes in the leaf was carried out in a time-dependent manner using a custom-rice whole genome 4£44K DNA microarray chip.…”

mentioning

confidence: 99%

“…That omicslevel approach thus provided an inventory of a large number of gamma radiation-responsive genes in rice. 13 Following on that major genome-wide study, we defined the objective of the current study as to analyze the differential expression of proteins in irradiated rice leaves at the level of the proteome. For this purpose, we employed the highly sensitive 2-D differential gel electrophoresis (2-DIGE) technique, which is a very useful and reliable method for detecting changes in expression between 2 samples of the same genome.…”

mentioning

confidence: 99%

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2D-DIGE-based proteome expression changes in leaves of rice seedlings exposed to low-level gamma radiation at Iitate village, Fukushima

Hayashi

Moro

Rohila

et al. 2015

Plant Signaling & Behavior

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The present study continues our previous research on investigating the biological effects of low-level gamma radiation in rice at the heavily contaminated Iitate village in Fukushima, by extending the experiments to unraveling the leaf proteome. 14-days-old plants of Japonica rice (Oryza sativa L. cv. Nipponbare) were subjected to gamma radiation level of upto 4 mSv/h, for 72 h. Following exposure, leaf samples were taken from the around 190 mSv/3 d exposed seedling and total proteins were extracted. The gamma irradiated leaf and control leaf (harvested at the start of the experiment) protein lysates were used in a 2-D differential gel electrophoresis (2D-DIGE) experiment using CyDye labeling in order to asses which spots were differentially represented, a novelty of the study. 2D-DIGE analysis revealed 91 spots with significantly different expression between samples (60 positive, 31 negative). MALDI-TOF and TOF/TOF mass spectrometry analyses revealed those as comprising of 59 different proteins (50 up-accumulated, 9 downaccumulated). The identified proteins were subdivided into 10 categories, according to their biological function, which indicated that the majority of the differentially expressed proteins consisted of the general (non-energy) metabolism and stress response categories. Proteome-wide data point to some effects of low-level gamma radiation exposure on the metabolism of rice leaves.

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“…The results of this analysis complement the ones previously obtained in the context of genome and transcriptome. 13 by adding a proteome dimension as well.…”

Section: Discussionmentioning

confidence: 99%

“…We could speculate that the considerable reduction in expression of photosynthesis proteins could be linked with the withering observed at the tips of the irradiated leaves. 13 …”

Section: Energymentioning

confidence: 99%

mentioning

confidence: 98%

mentioning

confidence: 99%

mentioning

confidence: 99%

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2D-DIGE-based proteome expression changes in leaves of rice seedlings exposed to low-level gamma radiation at Iitate village, Fukushima

Hayashi

Moro

Rohila

et al. 2015

Plant Signaling & Behavior

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Effects of environmental stress factors on the actin cytoskeleton of fungi and plants: Ionizing radiation and ROS

Stephan

2023

Cytoskeleton

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Actin is an abundant and multifaceted protein in eukaryotic cells that has been detected in the cytoplasm as well as in the nucleus. In cooperation with numerous interacting accessory‐proteins, monomeric actin (G‐actin) polymerizes into microfilaments (F‐actin) which constitute ubiquitous subcellular higher order structures. Considering the extensive spatial dimensions and multifunctionality of actin superarrays, the present study analyses the issue if and to what extent environmental stress factors, specifically ionizing radiation (IR) and reactive oxygen species (ROS), affect the cellular actin‐entity. In that context, this review particularly surveys IR‐response of fungi and plants. It examines in detail which actin‐related cellular constituents and molecular pathways are influenced by IR and related ROS. This comprehensive survey concludes that the general integrity of the total cellular actin cytoskeleton is a requirement for IR‐tolerance. Actin's functions in genome organization and nuclear events like chromatin remodeling, DNA‐repair, and transcription play a key role. Beyond that, it is highly significant that the macromolecular cytoplasmic and cortical actin‐frameworks are affected by IR as well. In response to IR, actin‐filament bundling proteins (fimbrins) are required to stabilize cables or patches. In addition, the actin‐associated factors mediating cellular polarity are essential for IR‐survivability. Moreover, it is concluded that a cellular homeostasis system comprising ROS, ROS‐scavengers, NADPH‐oxidases, and the actin cytoskeleton plays an essential role here. Consequently, besides the actin‐fraction which controls crucial genome‐integrity, also the portion which facilitates orderly cellular transport and polarized growth has to be maintained in order to survive IR.

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Fukushima's lessons from the blue butterfly: A risk assessment of the human living environment in the post-Fukushima era

Otaki

2016

Integr Environ Assess Manag

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A series of studies on the pale grass blue butterfly that were carried out to assess the biological effects of the Fukushima nuclear accident teach 3 important lessons. First, it is necessary to have an environmental indicator species, such as the pale grass blue butterfly in Japan, that is common (not endangered), shares a living environment (air, water, and soil) with humans, and is amenable to laboratory experiments. The monitoring of such indicator species before and immediately after a nuclear accident likely reflects acute impacts caused by initial exposure. To assess transgenerational and chronic effects, continuous monitoring over time is encouraged. Second, it is important to understand the actual health status of a polluted region and comprehend the whole picture of the pollution impacts, rather than focusing on the selected effects of radiation alone. In our butterfly experiments, plant leaves from Fukushima were fed to larval butterflies to access whole-body effects, focusing on survival rate and morphological abnormalities (rather than focusing on a specific disease or biochemical marker). Our results revealed that ionizing radiation is unlikely to be the exclusive source of environmental disturbances. Airborne particulate matter from a nuclear reactor, regardless of its radioactivity, is likely equally important. Finally, our butterfly experiments demonstrate that there is considerable variation in sensitivities to nuclear pollution within a single species or even within a local population. Based on these results, it is speculated that high pollution sensitivity in humans may be caused not only by low levels of functional DNA repair enzymes but also by immunological responses to particulate matter in the respiratory tract. These lessons from the pale grass blue butterfly should be integrated in studying future nuclear pollution events and decision making on nuclear and environmental policies at the local and international levels in the postFukushima era. Integr Environ Assess Manag 2016;12:667-672. © 2016 SETAC.

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Unraveling Low-Level Gamma Radiation-Responsive Changes in Expression of Early and Late Genes in Leaves of Rice Seedlings at litate Village, Fukushima

Cited by 41 publications

References 38 publications

2D-DIGE-based proteome expression changes in leaves of rice seedlings exposed to low-level gamma radiation at Iitate village, Fukushima

2D-DIGE-based proteome expression changes in leaves of rice seedlings exposed to low-level gamma radiation at Iitate village, Fukushima

Effects of environmental stress factors on the actin cytoskeleton of fungi and plants: Ionizing radiation and ROS

Fukushima's lessons from the blue butterfly: A risk assessment of the human living environment in the post-Fukushima era

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