Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches

Yazicioglu, Yavuz F.; Aksoylar, Halil‐Ibrahim; Pal, Rinku; Patsoukis, Nikolaos; Boussiotis, Vassiliki A.

doi:10.3791/58490

Cited by 2 publications

(2 citation statements)

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“…Flow cytometry was performed as described previously 54 . Briefly, collected spleens were injected with ice-cold PBS and mashed through a 70-μm strainer (Falcon) or crushed between the rough ends of microscope slides.…”

Section: Flow Cytometry and Imaging Flow Cytometrymentioning

confidence: 99%

Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis

et al. 2023

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Germinal center (GC) B cells undergo proliferation at very high rates in a hypoxic microenvironment but the cellular processes driving this are incompletely understood. Here we show that the mitochondria of GC B cells are highly dynamic, with significantly upregulated transcription and translation rates associated with the activity of transcription factor A, mitochondrial (TFAM). TFAM, while also necessary for normal B cell development, is required for entry of activated GC precursor B cells into the germinal center reaction; deletion of Tfam significantly impairs GC formation, function and output. Loss of TFAM in B cells compromises the actin cytoskeleton and impairs cellular motility of GC B cells in response to chemokine signaling, leading to their spatial disorganization. We show that B cell lymphoma substantially increases mitochondrial translation and that deletion of Tfam in B cells is protective against the development of lymphoma in a c-Myc transgenic mouse model. Finally, we show that pharmacological inhibition of mitochondrial transcription and translation inhibits growth of GC-derived human lymphoma cells and induces similar defects in the actin cytoskeleton.

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Section: Flow Cytometry and Imaging Flow Cytometrymentioning

confidence: 99%

Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis

et al. 2023

Self Cite

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“…Flow cytometry was performed as previously described (Yazicioglu et al, 2018). Briefly, spleens were injected with ice cold PBS and mashed through a 70 µm strainer or crushed between the frosted ends of microscope slides.…”

Section: Flow Cytometry and Imagestreammentioning

confidence: 99%

Dynamic mitochondrial transcription and translation in B cells control germinal centre entry and lymphomagenesis

Yazicioglu

Marin

Galiani

et al. 2022

Preprint

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Germinal centre (GC) B cells undergo proliferation at very high rates in a hypoxic microenvironment, but the cellular processes driving this are incompletely understood. Here we show that the mitochondria of GC B cells are highly dynamic, with significantly upregulated transcription and translation rates associated with the activity of transcription factor mitochondrial A (Tfam). Tfam, whilst also necessary for normal B cell development, is required for entry of activated GC-precursor B cells into the germinal centre reaction, and deletion of Tfam significantly impairs GC formation, function, and output. Loss of Tfam in B cells compromises the actin cytoskeleton and impairs cellular motility of GC B cells in response to chemokine signalling, leading to their spatial disorganisation. We show that B cell lymphoma substantially increases mitochondrial translation, and deletion of Tfam in B cells is protective against the development of lymphoma in a c-Myc transgenic model. Finally, we show that pharmacologic inhibition of mitochondrial transcription inhibits growth of GC-derived human lymphoma cells, and induces similar defects in the actin cytoskeleton.

show abstract

Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches

Cited by 2 publications

References 0 publications

Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis

Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis

Dynamic mitochondrial transcription and translation in B cells control germinal centre entry and lymphomagenesis

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