Unraveling global and diazotrophic bacteriomes of boreal forest floor feather mosses and their environmental drivers at the ecosystem and at the plant scale in North America

Renaudin, Marie; Laforest‐Lapointe, Isabelle; Bellenger, Jean‐Philippe

doi:10.1016/j.scitotenv.2022.155761

Cited by 14 publications

(14 citation statements)

References 99 publications

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“…They showed that Cyanobacteria accounted for <10% of the bacterial communities while Proteobacteria followed by Acidobacteria , and Actinobacteria were the most abundant bacterial phyla in boreal moss species. Our findings are in contrast with recent studies that have shown sequence read dominance of the members of the phyla Cyanobacteria in the diazotrophic bacteriome of moss in Canadian boreal forests (Renaudin et al, 2022b). These disparities in diazotrophic diversity are most likely attributed to the use of different primer pairs and the primer degeneracy of the PolF/PolR primers, as previously addressed (Gaby & Buckley, 2012).…”

Section: Discussioncontrasting

confidence: 99%

“…According to our study, there may be various types of metabolically active diazotrophs, beyond the phylum Cyanobacteria that can perform BNF in mosses (Figure 2). This contradicts the common assumption that Cyanobacteria are the primary N 2 -fixers in mosses (Holland-Moritz et al, 2021;Ininbergs et al, 2011;Leppänen et al, 2013;Renaudin et al, 2022b;Warshan et al, 2016). It is important to note that although the abundance of N 2 -fixers and BNF rates is not necessarily linked, even a low abundance of N 2 -fixers can contribute significantly to high BNF rates (Warshan et al, 2016).…”

Section: Frankia Dominates the Diazotrophic Communitymentioning

confidence: 89%

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Biological nitrogen fixation, diversity and community structure of diazotrophs in two mosses in 25 temperate forests

Groß,

Hossen,

Dittrich

et al. 2023

Environmental Microbiology

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Many moss species are associated with nitrogen (N)‐fixing bacteria (diazotrophs) that support the N supply of mosses. Our knowledge relates primarily to pristine ecosystems with low atmospheric N input, but knowledge of biological N fixation (BNF) and diazotrophic communities in mosses in temperate forests with high N deposition is limited. We measured BNF rates using the direct stable isotope method and studied the total and potentially active diazotrophic communities in two abundant mosses, Brachythecium rutabulum and Hypnum cupressiforme, both growing on lying deadwood trunks in 25 temperate forest sites. BNF rates in both mosses were similar to those observed in moss species of pristine ecosystems. H. cupressiforme fixed three times more N2 and exhibited lower diazotrophic richness than B. rutabulum. Frankia was the most prominent diazotroph followed by cyanobacteria Nostoc. Manganese, iron, and molybdenum contents in mosses were positively correlated with BNF and diazotrophic communities. Frankia maintained high BNF rates in H. cupressiforme and B. rutabulum even under high chronic N deposition in Central European forests. Moss N concentration and 15N abundance indicate a rather minor contribution of BNF to the N nutrition of these mosses.

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Section: Discussioncontrasting

confidence: 99%

Section: Frankia Dominates the Diazotrophic Communitymentioning

confidence: 89%

Biological nitrogen fixation, diversity and community structure of diazotrophs in two mosses in 25 temperate forests

Groß,

Hossen,

Dittrich

et al. 2023

Environmental Microbiology

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“…Rather, this finding could be a caveat of metagenomic analysis on DNA. Cyanobacterial DNA may linger in brown sections, though the actual cells could be dormant or even dead, not actively fixing nitrogen as shown by in Renaudin et al (2022b), whose work was based on cDNA and nifh sequencing. Further quantitative analyses may reveal true differences in activity between different moss sections.…”

Section: Moss Microbial Community Assembly Varies Strongly Between Mo...mentioning

confidence: 99%

Boreal moss-microbe interactions are revealed through metagenome assembly of novel bacterial species

Ishak

Faticov

Rondeau-Leclaire

et al. 2023

Preprint

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- Moss microbial communities play important roles for ecosystem processes in boreal forests. Host identity and leaf litter presence can affect microbial community structures of mosses. However, the extent to which host-specific characteristics and land use type affect taxonomic and functional profiles of microbial communities of boreal mosses is still poorly understood. - We used shotgun metagenomic sequencing to characterize microbial taxonomy and metabolic KEGG ortholog (KO) profiles between green and brown sections of five moss species across different natural sites, and two moss species between a natural site and a mine site in Eeyou Istchee, Quebec, Canada. - Our results demonstrate that the abundance of nitrogen metabolic genes differ between moss sections and that mosses from natural and mine environment associate with different microbial taxa and KO profiles. Importantly, conditions at the mine site appear to favor microbial taxa that can tolerate perturbated environments, including taxa that can oxidize sulfur and participate in biofilm formation. - Overall, our results highlight that moss section, moss species identity, and land-use type are strong drivers of diversity and community structure of moss-associated microbial taxa and metabolic genes. These findings could have major implications for boreal forests facing climate change and anthropogenic pressures

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“…Bryophyte-bacterial communities seem to be shaped by the host species. For example, in boreal forests, some bacterial groups are frequent and abundant on feather and peat mosses suggesting a species-specific relationship (Bragina et al 2015;Kostka et al 2016;Jean et al 2020;Holland-Moritz et al 2021;Renaudin et al 2022b). The environment also affects the bacterial composition of host species and may account for community differences between habitats (Bouchard et al 2020;Jean et al 2020;Rodríguez Rodríguez et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Differentially abundant bacteria drive the N₂-fixation of a widespread moss in the forest-tundra transition zone

Escolástico-Ortiz

Blasi

Bellenger

et al. 2023

Preprint

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Bryophytes maintain symbiosis with epiphytic bacteria influencing the local nutrient budget. Moss bacterial communities are composed of a core microbiome and bacteria recruited from environmental sources. Notably, symbiotic N2-fixing bacteria contribute to the N budget in northern ecosystems through biological nitrogen fixation. This process may be affected by the abundance of diazotrophs and moss nutrient content. We used the abundant mossRacomitrium lanuginosumin a forest tundra and shrub tundra in Northern Quebec, Canada, to investigate the bacterial and diazotrophic communities associated with habitat type using amplicon sequencing of the bacterial 16S rRNA andnifHgenes and test whether the moss core microbiome has recruitment from the soil bacteria community. ThenifHamplicons and element analysis were used to test the effect of diazotrophic abundance and moss nutrient content on N2-fixation activity estimated by acetylene reduction assays. Moss microbial communities between tundra types hosted similar bacterial diversity but differentially abundant groups. The core microbiome ofR. lanuginosumis composed of bacteria strongly associated with northern mosses with no significant recruitment from the soil. The relative abundances of dominant diazotrophs are significantly correlated with acetylene reduction rates. In contrast, the moss nutrient content did not significantly drive N2-fixation. The proteobacterial generaAzorhizobiumandRhodomicrobiumrepresent newly reported bacteria associated with N2-fixation rates in the tundra. We identified critical bacterial groups related to moss-bacterial symbiosis and N2-fixation in the forest-tundra transition zone, a changing environment susceptible to climate warming.

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Unraveling global and diazotrophic bacteriomes of boreal forest floor feather mosses and their environmental drivers at the ecosystem and at the plant scale in North America

Cited by 14 publications

References 99 publications

Biological nitrogen fixation, diversity and community structure of diazotrophs in two mosses in 25 temperate forests

Biological nitrogen fixation, diversity and community structure of diazotrophs in two mosses in 25 temperate forests

Boreal moss-microbe interactions are revealed through metagenome assembly of novel bacterial species

Differentially abundant bacteria drive the N₂-fixation of a widespread moss in the forest-tundra transition zone

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Unraveling global and diazotrophic bacteriomes of boreal forest floor feather mosses and their environmental drivers at the ecosystem and at the plant scale in North America

Cited by 14 publications

References 99 publications

Biological nitrogen fixation, diversity and community structure of diazotrophs in two mosses in 25 temperate forests

Biological nitrogen fixation, diversity and community structure of diazotrophs in two mosses in 25 temperate forests

Boreal moss-microbe interactions are revealed through metagenome assembly of novel bacterial species

Differentially abundant bacteria drive the N2-fixation of a widespread moss in the forest-tundra transition zone

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Differentially abundant bacteria drive the N₂-fixation of a widespread moss in the forest-tundra transition zone