Unraveling Burst Selection Bias in Single-Molecule FRET of Species with Unequal Brightness and Diffusivity
Irina V. Gopich,
Hoi Sung Chung
Abstract:Single-molecule free diffusion experiments enable accurate quantification of coexisting species or states. However, unequal brightness and diffusivity introduce a burst selection bias and affect the interpretation of experimental results. We address this issue with a photon-by-photon maximum likelihood method, burstML, which explicitly considers burst selection criteria. BurstML accurately estimates parameters, including photon count rates, diffusion times, Forster resonance energy transfer (FRET) efficiencies… Show more
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