Unlocked Nucleic Acids for miRNA detection using two dimensional nano-graphene oxide

Robertson, Neil; Toscano, Amy E.; LaMantia, Vincent E.; Hizir, Mustafa Salih; Rana, Muhit; Balcioglu, Mustafa; Sheng, Jia; Yigit, Mehmet V.

doi:10.1016/j.bios.2016.02.058

Cited by 31 publications

(27 citation statements)

References 35 publications

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“…Thus, the fluorophore labeled probes move away from the graphene surface, and fluorescence can be measured (Zhu et al, 2015). Other research groups have coupled this approach with more elaborated probe designs such as UNA (unlocked nucleic acid) bases with improved selectivity (Robertson et al, 2017). Along these lines, lateral flow immunoassay analysis (LFIA), also called lateral flow nucleic acid biosensor (LFNAB), can be preferred for microRNA quantification.…”

Section: Micro and Nanotechnologies For The Detection Of Mirnamentioning

confidence: 99%

Technological Challenges and Future Issues for the Detection of Circulating MicroRNAs in Patients With Cancer

et al. 2019

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In the era of precision medicine, the success of clinical trials, notably for patients diagnosed with cancer, strongly relies on biomarkers with pristine clinical value but also on robust and versatile analytical technologies to ensure proper patients' stratification and treatment. In this review, we will first address whether plasmatic and salivary microRNAs can be considered as a reliable source of biomarkers for cancer diagnosis and prognosis. We will then discuss the pre-analytical steps preceding miRNA quantification (from isolation to purification), and how such process could be biased and time-consuming. Next, we will review the most recent tools derived from micro- and nano-technologies for microRNA detection available to date and how they may compete with current standards. This review will prioritize publications using relevant biological samples. The significance of various physical transduction schemes (mechanical, optical, electrical, etc.) for biological detection will be compared, and pros and cons of each method will be widely discussed. Finally, we will debate on how micro and nanotechnologies could widespread the use of biomarkers in modern medicine, to help manage patients with serious diseases such as cancer.

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Section: Micro and Nanotechnologies For The Detection Of Mirnamentioning

confidence: 99%

Technological Challenges and Future Issues for the Detection of Circulating MicroRNAs in Patients With Cancer

et al. 2019

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“…More recently, UNA modification has proven to be an interesting substitution for mismatch discrimination within miRNAs families. Robertson et al [124]. demonstrated the properties of UNA-AMOs for detecting specifically overexpressed miR10b in metastatic breast cancer.…”

Section: Mirna Detection Using Amosmentioning

confidence: 99%

Anti-miRNA oligonucleotides: A comprehensive guide for design

et al. 2018

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MicroRNAs (miRNAs) are small, non-coding RNA molecules that regulate gene expression post-transcriptionally. As a consequence of their function towards mRNA, miRNAs are widely associated with the pathogenesis of several human diseases, making miRNAs a target for new therapeutic strategies based on the control of their expression. Indeed, numerous works were published in the past decades showing the potential use of antisense oligonucleotides to target aberrant miRNAs (AMOs) involved in several human pathologies. New classes of chemical-modified-AMOs, including locked nucleic acid oligonucleotides, have recently proved their worth in silencing miRNAs. A correct design of a specific AMOs can help to improve their performance and potency towards the target miRNA by increasing for instance nuclease resistance and target affinity. This review outlines the technologies involved to suppress aberrant miRNAs. From the design strategies used in AMOs to its application in novel miRNA-based therapeutics and detection methodologies.

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“…With the helicase assistance, the detection duration was shorted, and the sensitivity was increased compared with the sensing platform without helicase. Similar work was also reported by Robertson et al: in their study, unlocked nucleic acids were used to discriminate a breast cancer miRNA from two other miRNAs . The test results showed that the fluorescence signal was further enhanced with the use of the highly specific endonuclease dsDNase for an enzymatic amplification step and the background signal was not affected.…”

Section: State‐of‐the‐art Protocols For High‐quality Mirna Detectionmentioning

confidence: 99%

“…To overcome these problems, nonenzymatic amplification methods have shown great potential in nucleic acid detection . Hybridization chain reaction (HCR), one of the most attractive enzyme‐free approaches, was used to develop a new enzyme‐free signal amplification‐based assay for miRNA detection, wherein the surface of GO was utilized as a fluorescence signal readout pathway . As shown in Figure A, miRNAs could efficiently initiate HCR between two species of fluorescent hairpin probes.…”

Section: State‐of‐the‐art Protocols For High‐quality Mirna Detectionmentioning

confidence: 99%

Progress in miRNA Detection Using Graphene Material–Based Biosensors

Zhang

Miao

Sun

et al. 2019

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MicroRNAs (miRNAs) are short, endogenous, noncoding RNAs that play critical roles in physiologic and pathologic processes and are vital biomarkers for several disease diagnostics and therapeutics. Therefore, rapid, low‐cost, sensitive, and selective detection of miRNAs is of paramount importance and has aroused increasing attention in the field of medical research. Among the various reported miRNA sensors, devices based on graphene and its derivatives, which form functional supramolecular nanoassemblies of π‐conjugated molecules, have been revealed to have great potential due to their extraordinary electrical, chemical, optical, mechanical, and structural properties. This Review critically and comprehensively summarizes the recent progress in miRNA detection based on graphene and its derivative materials, with an emphasis on i) the underlying working principles of these types of sensors, and the unique roles and advantages of graphene materials; ii) state‐of‐the‐art protocols recently developed for high‐performance miRNA sensing, including representative examples; and iii) perspectives and current challenges for graphene sensors. This Review intends to provide readers with a deep understanding of the design and future of miRNA detection devices.

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Unlocked Nucleic Acids for miRNA detection using two dimensional nano-graphene oxide

Cited by 31 publications

References 35 publications

Technological Challenges and Future Issues for the Detection of Circulating MicroRNAs in Patients With Cancer

Technological Challenges and Future Issues for the Detection of Circulating MicroRNAs in Patients With Cancer

Anti-miRNA oligonucleotides: A comprehensive guide for design

Progress in miRNA Detection Using Graphene Material–Based Biosensors

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