University of Wisconsin vs normal saline solutions for preservation of blood vessels of brain dead donors: A histopathological study

Abstract

The results of this in vitro study demonstrated that NS can be used as a worthy preserver for harvested vessels for up to 21 days, especially in resource-limited transplantation centers.

Recombinant human secretory leukocyte protease inhibitor ameliorated vessel preservation in experimentally isolated rat arteries

“…NSS is the most widely used preservative solution in vascular operative procedures (Kazemi et al, 2018;Weiss et al, 2009) as it is convenient and cheap. However, several studies have suggested unsatisfactory outcomes of its use, such as altered integrity of the endothelial layer and abolished vascular dilation (Weiss et al, 2009;Wilbring et al, 2011, Wilbring et al, 2013a, 2013b.…”

“…In H&E staining, the endothelial cells reveal as a flat layer of squamous-to-fusiform shape cells characterized by basophilic oval-shaped nucleus, which may protrude into the lumen of the blood vessel, and very few of eosinophilic cytoplasm. The vascular histopathology scoring criteria consisted of endothelial detachment, elastic membrane disruption, necrosis, endothelial degeneration/edema, and complete denudation (Kazemi et al, 2018). The severity score was graded as follows: grade 0 = no lesion, grade 1 = lesion less than 25% of tissue, and grade 2 = lesion more than 25% of tissue.…”

“…Vascular reconstruction is a therapeutic technique used in reconstructive arterial surgery for several pathological conditions including organ substitute, coronary artery bypass grafting in an ischemic heart (Ben Ali et al, 2018;Fahner et al, 2006;Kazemi et al, 2018;Wille et al, 2008), and infrarenal aortic replacement (Kieffer et al, 2004;Zatschler et al, 2009). A vascular allograft is the most attractive option for revascularization.…”

“…Several organ preservative solutions such as the University of Wisconsin (UW) solution, histidine-tryptophanketoglutarate (HTK) solution, and Celsior solution have been used in vascular transplantations (Kazemi et al, 2018). Cold 0.9% normal saline solution (NSS) is another preservative solution, which is used in some resource-limited (developing) countries (Kazemi et al, 2018). However, several studies have shown that NSS has a lower preservative efficacy when compared to other preservative solutions (Kazemi et al, 2018;Zatschler et al, 2009).…”

“…Cold 0.9% normal saline solution (NSS) is another preservative solution, which is used in some resource-limited (developing) countries (Kazemi et al, 2018). However, several studies have shown that NSS has a lower preservative efficacy when compared to other preservative solutions (Kazemi et al, 2018;Zatschler et al, 2009). Therefore, the addition or supplementation of a solution that could possibly enhance the preservative efficiency of NSS and prolong the vessel graft storage should be considered.…”