“Universal Warming” protocol for vitrified oocytes to streamline cell exchange for transnational donation programs: a multi-center study

Parmegiani, Lodovico; Minasi, MG; Arnone, A.; Casciani, Valentina; Cognigni, G.E.; Viñoles, Rita; Varricchio, Maria Teresa; Quintero, Luis Alberto Zarco; Greco, Ermanno; Filicori, Marco

doi:10.1007/s10815-020-01798-3

Cited by 14 publications

(3 citation statements)

References 26 publications

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“…As the protocol recommended by the vast majority of currently available thawing kits involves the thawing of vitri ed oocytes/embryos/blastocysts at 37°C and RT, which has resulted in successful outcomes [36][37][38], few studies have compared the advantages and disadvantages of this protocol with those of other temperature protocols. To the best of our knowledge, this is the rst study to assess the effects of two thawing procedures (all-37°C versus 37°C-RT) using the same thawing kit on the clinical outcomes of FET cycles.…”

Section: Discussionmentioning

confidence: 99%

An all-37 °C thawing method improves the clinical outcomes of vitrified frozen-thawed embryo transfer: a retrospective study using a case–control matching analysis

Yan

Yao

Yang

et al. 2023

Arch Gynecol Obstet

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Section: Discussionmentioning

confidence: 99%

An all-37 °C thawing method improves the clinical outcomes of vitrified frozen-thawed embryo transfer: a retrospective study using a case–control matching analysis

Yan

Yao

Yang

et al. 2023

Arch Gynecol Obstet

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“…This approach offers several advantages, including the ability to retrieve immature eggs without the need for hormonal stimulation, which is typically required for conventional ovulation induction and oocyte retrieval. Furthermore, ovarian cortex biopsies can be obtained quickly via laparoscopy, making this procedure suitable for prepubertal girls and adult patients with hormone-sensitive cancers who require urgent neoadjuvant anticancer therapies, such as chemotherapy or radiation treatments [3][4][5]. Regarding the success of ovarian cortex reimplantation (OCR) in achieving pregnancies, the first live birth after the reimplantation of autologous cryopreserved ovarian cortex using the slow freezing (SF) technique was reported in 2003 [1,6].…”

Section: Introductionmentioning

confidence: 99%

Optimizing the Ovarian Tissue Cryopreservation in the ‘Oncofertility’ Institutional Program at an Italian National Cancer Institute

Silvestris,

Minoia,

De Palma

et al. 2023

Healthcare

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Background: The majority of female cancer patients undergoing anticancer treatments are at risk of experiencing ‘cancer treatment-related infertility’, which can result in permanent damage to their reproductive prospects. Among the fertility preservation methods, ovarian tissue cryopreservation (OTC) has emerged as an alternative for these patients. The Cancer Institute of Bari initiated a research program to assess the feasibility of OTC. This study compares the viability of ovarian cortical fragments cryopreserved using slow freezing (SF) and ultra-rapid freezing (URF) methods. Methods: Ovarian cortex biopsies were obtained from 11 fertile women enrolled in our oncofertility service between June 2022 and January 2023. After tissue collection, a histological assessment was performed before cryopreservation. OTC was carried out using both SF and URF methods. Six months later, thawed samples were evaluated for follicle counts and histological integrity. Results: No statistically significant difference was observed in the proportion of intact follicles (means of 31.5% and 73.0% in the SF and URF groups, respectively; p = 0.064). However, there was a significant difference in the number of follicles between the SF group (n = 149) and the URF group (n = 37) (p = 0.046). Conclusions: We assessed the viability of ovarian cortex after freezing and thawing, focusing on the structural integrity of follicles. Our findings suggest that there are no significant differences between the SF and URF methods.

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“…In many IVF clinics, slow freezing has been superseded by vitrification due to superior survival rates [1][2][3][4] accompanied by considerable technical simplification. Additionally, with slow frozen cells, it was shown that applying rapid warming gives superior results compared to standard thawing protocols in terms of survival rates [5][6][7]. Preservation of oocytes and ovarian tissue cryopreservation (OTC) are two options for fertility protection in female cancer patients [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

The effect of high-throughput vitrification of human ovarian cortex tissue on follicular viability: a promising alternative to conventional slow freezing?

Schallmoser

Einenkel

Färber

et al. 2022

Arch Gynecol Obstet

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Background The standard procedure most frequently used for ovarian tissue cryopreservation (OTC) is slow freezing, while vitrification has been proposed as promising alternative and has built an impressive catalog of success in fertility laboratories regarding cryopreservation of oocytes and embryos. Methods We developed and evaluated a high-throughput protocol for vitrification of human ovarian tissue suitable for clinical processing. Follicular viability was assessed via calcein staining prior and after cryopreservation analyzing ovarian tissue of a cohort of 30 patients. Results We found no significant differences regarding follicular viability between slow frozen and vitrified cortex tissue samples 24 h after thawing and rapid warming. Follicular viability of thawed and rapid warmed samples was not significantly different in comparison to fresh samples, indicating high proportions of follicular survival rates with both methods. Conclusions High-throughput vitrification is a promising option in a clinical setting. More research is required to determine the status of other tissue-specific quality indicators potentially influencing on autotransplantation.

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“Universal Warming” protocol for vitrified oocytes to streamline cell exchange for transnational donation programs: a multi-center study

Cited by 14 publications

References 26 publications

An all-37 °C thawing method improves the clinical outcomes of vitrified frozen-thawed embryo transfer: a retrospective study using a case–control matching analysis

An all-37 °C thawing method improves the clinical outcomes of vitrified frozen-thawed embryo transfer: a retrospective study using a case–control matching analysis

Optimizing the Ovarian Tissue Cryopreservation in the ‘Oncofertility’ Institutional Program at an Italian National Cancer Institute

The effect of high-throughput vitrification of human ovarian cortex tissue on follicular viability: a promising alternative to conventional slow freezing?

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