Universal expression of cell adhesion molecule NCAM in neuroblastoma in contrast to L1: implications for different roles in tumor biology of neuroblastoma?

Wachowiak, Robin; Rawnaq, Tamina; Metzger, Roman; Quaas, Alexander; Fiegel, Henning C.; Kähler, Nils; Rolle, Udo; Izbicki, Jakob R.; Kaifi, Jussuf T.; Till, Holger

doi:10.1007/s00383-008-2264-z

Cited by 28 publications

(20 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown in Figure 1AB, FISH analysis performed on GD2 positive cell preparations from two patients with MYCN amplification showed presence of MYCN amplification. Cytospins of GD2 positive cell preparations were enriched in mononuclear NB cells expressing the NB-specific markers GD2, CD56 [15] and NB84 [16] (Figure 1C, D and E, respectively). Cytofluorimetric analysis showed that less than 5% of the cells recovered by GD2-positive immunomagnetic bead manipulation expressed CD45, whereas more than 95% expressed the B7H3 antigen (Figure 1F and G, respectively).…”

Section: Resultsmentioning

confidence: 98%

Bone Marrow-Infiltrating Human Neuroblastoma Cells Express High Levels of Calprotectin and HLA-G Proteins

Morandi

Scaruffi²,

Gallo³

et al. 2012

PLoS ONE

View full text Add to dashboard Cite

Metastases in the bone marrow (BM) are grim prognostic factors in patients with neuroblastoma (NB). In spite of extensive analysis of primary tumor cells from high- and low-risk NB patients, a characterization of freshly isolated BM-infiltrating metastatic NB cells is still lacking. Our aim was to identify proteins specifically expressed by metastatic NB cells, that may be relevant for prognostic and therapeutic purposes. Sixty-six Italian children over 18 months of age, diagnosed with stage 4 NB, were included in the study. Metastatic NB cells were freshly isolated from patients' BM by positive immunomagnetic bead manipulation using anti-GD2 monoclonal antibody. Gene expression profiles were compared with those obtained from archived NB primary tumors from patients with 5y-follow-up. After validation by RT-qPCR, expression/secretion of the proteins encoded by the up-regulated genes in the BM-infiltrating NB cells was evaluated by flow cytometry and ELISA. Compared to primary tumor cells, BM-infiltrating NB cells down-modulated the expression of CX3CL1, AGT, ATP1A2 mRNAs, whereas they up-regulated several genes commonly expressed by various lineages of BM resident cells. BM-infiltrating NB cells expressed indeed the proteins encoded by the top-ranked genes, S100A8 and A9 (calprotectin), CD177 and CD3, and secreted the CXCL7 chemokine. BM-infiltrating NB cells also expressed CD271 and HLA-G. We have identified proteins specifically expressed by BM-infiltrating NB cells. Among them, calprotectin, a potent inflammatory protein, and HLA-G, endowed with tolerogenic properties facilitating tumor escape from host immune response, may represent novel biomarkers and/or targets for therapeutic intervention in high-risk NB patients.

show abstract

Section: Resultsmentioning

confidence: 98%

Bone Marrow-Infiltrating Human Neuroblastoma Cells Express High Levels of Calprotectin and HLA-G Proteins

Morandi

Scaruffi²,

Gallo³

et al. 2012

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Most Oct-4 + and Oct-4 − cells expressed VEGF-R2 (Supplementary information, Figure S1, [13][14][15] [8], and the neuroblastic markers GD2 (Supplementary information, Figure S1, 9-11), CD56 (Supplementary information, Figure S1, [17][18][19] [8,49,50] and NB84 ( Figure 2A). All isotypic controls used for immunofluorescence tested negative ( Figure 2B Figure S1, 4,8,12,16,20).…”

Section: Identification and Immunophenotypic Characterization Of Oct-mentioning

confidence: 99%

“…At variance with TEC, bulk tumor cells did not express PSMA, vWF, CD105, HLA-class I or HLA-G ( Figure 6A). Most TECs were found to express the NB-associated markers GD2 [49] NB84 [41], and CD56 [50] ( Figure 6B, 6-8), which were shared by most tumor cells ( Figure 6A). …”

Section: Kinetics Of Human and Mouse Em Formation In Orthotopic Tumormentioning

confidence: 99%

Oct-4+/Tenascin C+ neuroblastoma cells serve as progenitors of tumor-derived endothelial cells

Pezzolo¹,

Parodi²,

Marimpietri³

et al. 2011

Cell Res

View full text Add to dashboard Cite

show abstract

“…In contrast to that in human glioblastomas, an association of L1 positivity with greater event-free and overall survival has been observed in patients with neuroblastoma. L1 negativity is independently prognostic of event-free and overall survival, and predicts a good outcome in children with neuroblastoma (15,16). Thus, L1 may function differently in the two tumor types.…”

Section: Introductionmentioning

confidence: 99%

Comparison of L1 expression and secretion in glioblastoma and neuroblastoma cells

Zhao

2012

Oncology Letters

View full text Add to dashboard Cite

Abstract. The expression of cell adhesion molecule L1 has been identified in a vast spectrum of tumors; however, its expression pattern with regard to tumor type is rarely discussed. In the present study, we studied L1 levels in human glioblastomas and neuroblastomas, and compared the expression and secretion of L1 in human glioblastoma U87-MG and neuroblastoma SK-N-SH cells. Immunofluorescence staining revealed different grades of L1 staining in human glioblastoma and neuroblastoma samples. In U87-MG cells, full-length L1 was weakly detected in cell lysates (CLs), while greater levels of abundant soluble L1 were confined in conditioned culture medium (CCM). In contrast, higher levels of full-length L1 were confined in SK-N-SH CLs, while almost no soluble forms of L1 were detected in CCM. Our data indicates various expression patterns of L1 in U87-MG and SK-N-SH cells, which may underlie the different malignancies of the two neural tumor types and further stress the importance of soluble L1-mediated signaling pathways in cell malignancy.

show abstract

Universal expression of cell adhesion molecule NCAM in neuroblastoma in contrast to L1: implications for different roles in tumor biology of neuroblastoma?

Cited by 28 publications

References 17 publications

Bone Marrow-Infiltrating Human Neuroblastoma Cells Express High Levels of Calprotectin and HLA-G Proteins

Bone Marrow-Infiltrating Human Neuroblastoma Cells Express High Levels of Calprotectin and HLA-G Proteins

Oct-4+/Tenascin C+ neuroblastoma cells serve as progenitors of tumor-derived endothelial cells

Comparison of L1 expression and secretion in glioblastoma and neuroblastoma cells

Contact Info

Product

Resources

About