Universal and Programmable Rolling Circle Amplification-CRISPR/Cas12a-Mediated Immobilization-Free Electrochemical Biosensor

Qing, Min; Chen, Sheng Liang; Sun, Zhe; Fan, Yi; Luo, Hong Qun; Li, Nian Bing

doi:10.1021/acs.analchem.1c00805

Cited by 112 publications

(65 citation statements)

References 40 publications

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“…CRISPR-based biosensors also showed promising potential for the detection of non-infectious human diseases, such as cancer, based on the features with disease-related gene mutations, singlenucleotide polymorphism, DNA methylation, and so on (Aman et al, 2020;Li B. et al, 2021;Wang et al, 2021c;Sheng et al, 2021). For example, a Cas12a-based biosensor showed sensitive detection of gene-PIK3CA H1047R mutation low at 0.001%, which has great potential to predict early-stage breast cancer (Deng et al, 2021).…”

Section: Detection Of Non-infectious Diseasesmentioning

confidence: 99%

“…For example, a Cas12a-based biosensor showed sensitive detection of gene-PIK3CA H1047R mutation low at 0.001%, which has great potential to predict early-stage breast cancer (Deng et al, 2021). A Cas12a-based transcription factor detection method showed an LoD of 0.2 pM for NF-kappaB p50 subunit from cancer cell samples, which can be further applied for physical dysfunction monitoring and drug screening (Li B. et al, 2021). Recent work introduced a hairpin probe to preserve the analytical fidelity and developed a CRISPR/Cas9-triggered hairpin probe-mediated biosensing method, termed the CHP system, specifically initiating double isothermal amplifications when Cas9-mediated cleavage occurs and the hairpin probe recognizes the original sequences (Wang M. et al, 2021).…”

Section: Detection Of Non-infectious Diseasesmentioning

confidence: 99%

“…Combined with a microfluidic chip, the CRISPR/Cas9 system can be developed for rapid and efficient kinase screening and further to separate cells according to different deformability, with flexible cells flowing out and stiff cells remaining trapped (Han et al, 2016). Recently, immunoassays based on CRISPR/Cas systems for the sensitive and rapid detection of protein targets were also explored, which is akin to the traditional ELISA (Zhao Q. et al, 2021;Li H. et al, 2021). Li et al designed a series of aptamer-flanked activator DNA strands to correlate non-nucleic acid analytes with the CRISPR/Cas12a system, enabling an ultrasensitive detection (Li H. et al, 2021).…”

Section: Sensing For Non-nucleic Acid Targetsmentioning

confidence: 99%

“…Recently, immunoassays based on CRISPR/Cas systems for the sensitive and rapid detection of protein targets were also explored, which is akin to the traditional ELISA (Zhao Q. et al, 2021;Li H. et al, 2021). Li et al designed a series of aptamer-flanked activator DNA strands to correlate non-nucleic acid analytes with the CRISPR/Cas12a system, enabling an ultrasensitive detection (Li H. et al, 2021). Zhao et al employed DNA-AuNPs to establish the signal transduction between trans-cleavage of CRISPR/Cas12a and protein analytes and showed a quantitative level of attomolar, 1,000-fold more sensitive and 15-fold wider detection range than traditional ELISA (Zhao Q. et al, 2021).…”

Section: Sensing For Non-nucleic Acid Targetsmentioning

confidence: 99%

“…Currently, clustered regularly interspaced short palindromic repeats (CRISPR) based biosensors have been developed as powerful tools for nucleic acid sensing and widely applied to the rapid diagnosis of infectious pathogens (Kellner et al, 2019;Qin et al, 2019;Broughton et al, 2020;Ding R. et al, 2021;Song F. et al, 2021;Chen et al, 2021c;Escalona-Noguero et al, 2021;Ge et al, 2021) and the detection of DNA or miRNAs associated with diseases such as cancer (Li B. et al, 2021;Wang et al, 2021c;. These biosensors rely on the CRISPR systems containing CRISPR-associated proteins (Cas) with nonspecific endonuclease activity to efficiently cleave specific targets via guide RNAs (gRNAs) (Cong et al, 2013;Mali et al, 2013;van der Oost, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Powerful CRISPR-Based Biosensing Techniques and Their Integration With Microfluidic Platforms

Chen

et al. 2022

Front. Bioeng. Biotechnol.

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In the fight against the worldwide pandemic coronavirus disease 2019 (COVID-19), simple, rapid, and sensitive tools for nucleic acid detection are in urgent need. PCR has been a classic method for nucleic acid detection with high sensitivity and specificity. However, this method still has essential limitations due to the dependence on thermal cycling, which requires costly equipment, professional technicians, and long turnover times. Currently, clustered regularly interspaced short palindromic repeats (CRISPR)-based biosensors have been developed as powerful tools for nucleic acid detection. Moreover, the CRISPR method can be performed at physiological temperature, meaning that it is easy to assemble into point-of-care devices. Microfluidic chips hold promises to integrate sample processing and analysis on a chip, reducing the consumption of sample and reagent and increasing the detection throughput. This review provides an overview of recent advances in the development of CRISPR-based biosensing techniques and their perfect combination with microfluidic platforms. New opportunities and challenges for the improvement of specificity and efficiency signal amplification are outlined. Furthermore, their various applications in healthcare, animal husbandry, agriculture, and forestry are discussed.

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Section: Detection Of Non-infectious Diseasesmentioning

confidence: 99%

Section: Detection Of Non-infectious Diseasesmentioning

confidence: 99%

Section: Sensing For Non-nucleic Acid Targetsmentioning

confidence: 99%

Section: Sensing For Non-nucleic Acid Targetsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Powerful CRISPR-Based Biosensing Techniques and Their Integration With Microfluidic Platforms

Chen

et al. 2022

Front. Bioeng. Biotechnol.

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Materialistic Interfaces with Nucleic Acids: Principles and Their Impact

Zhang

et al. 2022

Adv Funct Materials

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Mendelian genetics spark the century‐old revolution in genetic engineering. DNA structural engineering may well spark a similar revolution in chemistry. The first few man‐made DNA structures are purely DNA but increased interest in hybrid DNA with other molecules is regaining popularity. Nucleic acids can interact with non‐nucleic acid‐based materials, showing their materialistic flexibility and potential and the prospect of forming new future materials. This review provides a deep materialistic background based on the interaction of nucleic acids with these non‐nucleic acid‐based materials covering comprehensive topics. The non‐nucleic acid materials are broadly divided into biological materials, for example, small‐biomolecules, peptides, proteins, and lipids, and non‐biological materials consisting of metal ions, chemical molecules, low‐dimensional nanomaterials, and micro‐scale polymers. Of significance, combining the unique properties of nucleic acids with these non‐genetic materials can bring about surprising synergistic characteristics and drive attractive applications in bio‐imaging, chem/bio‐sensing, disease therapies, antimicrobial works, and even information storage.

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Clustered Regularly Interspaced short palindromic repeats‐Based Microfluidic System in Infectious Diseases Diagnosis: Current Status, Challenges, and Perspectives

Xie

Chen

et al. 2022

Advanced Science

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Mitigating the spread of global infectious diseases requires rapid and accurate diagnostic tools. Conventional diagnostic techniques for infectious diseases typically require sophisticated equipment and are time consuming. Emerging clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) detection systems have shown remarkable potential as next-generation diagnostic tools to achieve rapid, sensitive, specific, and field-deployable diagnoses of infectious diseases, based on state-of-the-art microfluidic platforms. Therefore, a review of recent advances in CRISPR-based microfluidic systems for infectious diseases diagnosis is urgently required. This review highlights the mechanisms of CRISPR/Cas biosensing and cutting-edge microfluidic devices including paper, digital, and integrated wearable platforms. Strategies to simplify sample pretreatment, improve diagnostic performance, and achieve integrated detection are discussed. Current challenges and future perspectives contributing to the development of more effective CRISPR-based microfluidic diagnostic systems are also proposed.

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Universal and Programmable Rolling Circle Amplification-CRISPR/Cas12a-Mediated Immobilization-Free Electrochemical Biosensor

Cited by 112 publications

References 40 publications

Powerful CRISPR-Based Biosensing Techniques and Their Integration With Microfluidic Platforms

Powerful CRISPR-Based Biosensing Techniques and Their Integration With Microfluidic Platforms

Materialistic Interfaces with Nucleic Acids: Principles and Their Impact

Clustered Regularly Interspaced short palindromic repeats‐Based Microfluidic System in Infectious Diseases Diagnosis: Current Status, Challenges, and Perspectives

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