Nitrogen (N) is the primary limiting factor to increase wheat (Triticum aestivum L.) yield in Brazil, and the use of N-fi xing agents might be an alternative to increase wheat production. A fi eld experiment was conducted in 2006 in São Paulo State, Brazil, with three N rates (0, 60, and 120 kg ha −1 ) and three endophytic bacteria (IAC-HT-11, IAC-HT-12, and IAC-AT-8) to evaluate their combined impact on biophysical (leaf area index [LAI] and grain yield) and spectral parameters (normalized diff erence vegetation index [NDVI], absorption band parameters) of wheat and to determine the spectral parameters that best correlated with LAI and grain yield. Leaf area index and canopy spectral refl ectance measurements were made at jointing, heading, and ripening growth stages for this purpose. Th e statistical relationships between biophysical and spectral parameters were evaluated within and among the treatments. Statistical results showed that the N rates signifi cantly aff ected biophysical and spectral parameters. Bacteria inoculation presented a signifi cant eff ect only on grain yield, which is consistent with results reported in the literature. Th e NDVI, followed by absorption band parameters P B and A B at 1205 nm, provided the best estimates of LAI and grain yield during the heading stage, indicating that it may be the best stage for remote sensing-based yield mapping.