Unique properties of [ ]MK-801 binding in membranes from the rat spinal cord

Liu, Y; Souverbie, Frédéric; Sundström, Erik; Euler, Gabriel von

doi:10.1016/s0006-8993(97)00207-2

Cited by 9 publications

(2 citation statements)

References 42 publications

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“…The regional heterogeneity of NMDA receptor function has previously been addressed by several research groups (Stone & Perkins 1981;Buller et al 1994;Liu et al 1997;Souverbie et al 1996), mainly with a focus on effects and affinities of agonists and antagonists. We decided to study the polyamine and redox modulation of [ 3 H]MK-801 binding to NMDA receptors in the spinal cord and cerebral cortex, as well as the possible interaction between these two modulatory mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…The regional pharmacological heterogeneity is also more complex, and in this respect the spinal cord is a suitable example. In previous studies we have found features of the spinal cord NMDA receptors unique to this region (Souverbie et al 1996;Liu et al 1997). As NMDA receptors seem to be critically involved in several normal functions as well as disease processes in the spinal cord (Gó mez-Pinilla et al 1989;Headley & Grillner 1990;Dickenson et al 1997), detailed knowledge of the pharmacology and subunit assembly of the native spinal cord NMDA receptors are of major importance.…”

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confidence: 97%

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Polyamine and Redox Modulation of [³H]MK‐801 Binding to N‐Methyl‐d‐aspartate Receptors in the Spinal Cord and Cerebral Cortex

Sundström

2001

Pharmacology & Toxicology

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Abstract:The pharmacology of N-methyl--aspartate (NMDA) receptors shows regional differences in affinity for various agonists and antagonists. We have investigated the modulatory mechanisms acting via the polyamine, redox and proton sites in the cerebral cortex and the spinal cord of adult, male rats using [ 3 H]MK-801 binding. The affinity for glycineindependent spermine stimulation was one magnitude higher in cerebrocortical than in spinal cord membranes while the affinity for the spermine antagonist arcaine was similar. Spermine abolished the inhibiting effect of low pH in both regions. Thus, the difference in the polyamine site between the two regions seems to be restricted to agonist binding. The proportion of high affinity/total ifenprodil binding was ∑35% both in the spinal cord and the cerebral cortex, suggesting similar relative amounts of the NMDA receptor subunit 2B. The affinity of ifenprodil to the high affinity site was however significantly higher in the cerebral cortex. Redox modulatory agents had similar effects in the two regions but spermine fully counteracted the inhibiting effect of 0.2 mM 5,5ø-dithio-bis(2-nitrobenzoic acid) (DTNB) in the cerebral cortex while there was only a partial effect in the spinal cord. These data show that the regional pharmacological heterogeneity involves several of the mechanisms regulating the function of the NMDA receptor. The data also indicate that the NMDA receptor subunit 2B is much more common in spinal cord than previously suggested.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 97%

Polyamine and Redox Modulation of [³H]MK‐801 Binding to N‐Methyl‐d‐aspartate Receptors in the Spinal Cord and Cerebral Cortex

Sundström

2001

Pharmacology & Toxicology

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Z-analysis: A new approach to analyze stimulation curves with intrinsic basal stimulation

Hedlund

Euler

2005

Biochemical Pharmacology

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Positive N-methyl-d-aspartate receptor modulation by selective glycine transporter-1 inhibition in the rat dorsal spinal cord in vivo

Whitehead

Pearce

Walker³

et al. 2004

Neuroscience

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Unique properties of [ ]MK-801 binding in membranes from the rat spinal cord

Cited by 9 publications

References 42 publications

Polyamine and Redox Modulation of [³H]MK‐801 Binding to N‐Methyl‐d‐aspartate Receptors in the Spinal Cord and Cerebral Cortex

Polyamine and Redox Modulation of [³H]MK‐801 Binding to N‐Methyl‐d‐aspartate Receptors in the Spinal Cord and Cerebral Cortex

Z-analysis: A new approach to analyze stimulation curves with intrinsic basal stimulation

Positive N-methyl-d-aspartate receptor modulation by selective glycine transporter-1 inhibition in the rat dorsal spinal cord in vivo

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Unique properties of [ ]MK-801 binding in membranes from the rat spinal cord

Cited by 9 publications

References 42 publications

Polyamine and Redox Modulation of [3H]MK‐801 Binding to N‐Methyl‐d‐aspartate Receptors in the Spinal Cord and Cerebral Cortex

Polyamine and Redox Modulation of [3H]MK‐801 Binding to N‐Methyl‐d‐aspartate Receptors in the Spinal Cord and Cerebral Cortex

Z-analysis: A new approach to analyze stimulation curves with intrinsic basal stimulation

Positive N-methyl-d-aspartate receptor modulation by selective glycine transporter-1 inhibition in the rat dorsal spinal cord in vivo

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Product

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Polyamine and Redox Modulation of [³H]MK‐801 Binding to N‐Methyl‐d‐aspartate Receptors in the Spinal Cord and Cerebral Cortex

Polyamine and Redox Modulation of [³H]MK‐801 Binding to N‐Methyl‐d‐aspartate Receptors in the Spinal Cord and Cerebral Cortex